Preparation and application of pig-derived probiotic compound

Abstract

The present invention provides a preparation of a pig-derived probiotic compound. The pig-derived probiotic compound is characterized in that the probiotic compound comprises the following components:bacillus subtilis, lactobacillus rhamnosus and lactobacillus plantarum; and the preparation of the compound comprises the following steps: step a, activating the bacillus subtilis, lactobacillus rhamnosus and lactobacillus plantarum, wherein concentration of a bacterial solution reaches (1-2)x10<9> cfu / ml in a logarithmic phase; step b, centrifuging the bacterial solution obtained in the step a,discarding a supernatant, and collecting bacterial sludge; and step c, uniformly mixing the bacterial sludge and a protective agent according to a mass ratio of 1:(2-5), conducting stirring to enablethe protective agent to be in full contact with bacterial body, and conducting freeze-drying under conditions of -20 DEG C to -50 DEG C and 50-80 Pa to form a powder preparation so as to obtain the probiotic compound. The probiotic compound is derived from healthy pig intestines and obtained by separating and culturing, has strong stress resistance, is not liable to inactivate, can significantly improve a barrier function of the pig intestines and regulate intestinal flora, and is especially suitable for weaned piglets.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to the preparation and application of a porcine-derived probiotic compound. Background technique [0002] Feed antibiotics have played a certain positive role in improving feed utilization and reducing disease occurrence. However, with the gradual recognition of their harm to livestock and poultry product quality, ecological environment and human health, it has become increasingly difficult to find green and pollution-free feed additives. become an urgent problem to be solved in the animal husbandry industry. [0003] Pig intestines contain a large number of different types of microorganisms such as probiotics and pathogenic bacteria. Under normal circumstances, the intestinal barrier function prevents bacteria and endotoxins from invading the body. In the current intensive pig raising process, there are a large number of different types of stress factors such as high density...

AI Technical Summary

Problems solved by technology

Probiotics such as Bifidobacteria can utilize oligosaccharides, while some harmful bacteria such as Salmonella and Staphylococcus aureus are difficult to utilize oligosaccharides for most prebiotics. Soybean saponins mainly exist in legumes, accounting for about Dry weight 0.6-6.2%. Soybean is one of the main raw materials for pig feed, but there are few reports on the screening of probiotic strains that make full use of soybean saponins

[0005] At present, there are many factors that restrict probiotic products from playing their due role. The main manifestations are that heterologous bacteria have poor ability to adapt to the environment, adhere to colonization and rapid reproduction, and the bacteria are single; It is easy to inactivate during processing, which is also a common key technical problem that restricts the popularization and application of current probiotic preparations

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