Synthetic method of mineralized sendai virus-based tumor combined treatment nano preparation

A technology of combined therapy and nano-preparation, applied in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of cumbersome process, harsh environmental requirements, pollution, etc. significant effect

Inactive Publication Date: 2020-02-25
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It solves the problem that the traditional synthesis method is too cumbersome, the environment is harsh, and it has certain pollution

Method used

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  • Synthetic method of mineralized sendai virus-based tumor combined treatment nano preparation
  • Synthetic method of mineralized sendai virus-based tumor combined treatment nano preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Accurately weigh 5 mg of indocyanine green (ICG) powder, add 1 mL of ultrapure water, and then ultrasonically dissolve to obtain an aqueous solution of ICG with a concentration of 5 mg / ml.

[0024] 2) Synthesis of (ICG+SEV)@CaHPO 4 The nanoparticle method is as follows:

[0025] (1) Place the single-mouth bottle containing 500ml of SEV solution on a magnetic stirrer, then add 25ul of the above-mentioned ICG dropwise under stirring conditions, and continue stirring after the dropwise addition;

[0026] (2) Configure CaCl with a concentration of 50mM 2 Solution, take 125ul and add dropwise to the above reaction system, and continue to stir for 15min after the dropwise addition;

[0027] (3) Configure Na with a concentration of 50mM 2 HPO 4 solution, and add 25ul dropwise to the reaction system, and continue to stir for 10min after the dropwise addition;

[0028] (4) After stirring, centrifuge at 10000rpm for 3min, then wash with 1ml double distilled water, continu...

Embodiment 2

[0030] 1) Accurately weigh 10 mg of indocyanine green (ICG) powder, add 1 mL of ultrapure water, and ultrasonically dissolve to obtain an aqueous solution of ICG with a concentration of 10 mg / ml.

[0031] 2) Synthesis of (ICG+SEV)@CaHPO 4 The nanoparticle method is as follows:

[0032] (1) Place the one-mouth bottle containing 1ml of SEV solution on a magnetic stirrer, then add 25ul of the above-mentioned ICG dropwise under stirring conditions, and continue stirring after the dropwise addition;

[0033] (2) Configure CaCl with a concentration of 100mM 2 Solution, take 125ul and add dropwise to the above reaction system, and continue to stir for 15min after the dropwise addition;

[0034] (3) Configure Na with a concentration of 100mM 2 HPO 4 solution, and add 25ul dropwise to the reaction system, and continue to stir for 10min after the dropwise addition;

[0035] (4) After stirring, centrifuge at 10000rpm for 3min, then wash with 1ml double distilled water, continue cent...

Embodiment 3

[0037] 1) Accurately weigh 20 mg of indocyanine green (ICG) powder, add 1 mL of ultrapure water, and then ultrasonically dissolve to obtain an aqueous solution of ICG with a concentration of 20 mg / ml.

[0038] 2) Synthesis of (ICG+SEV)@CaHPO 4 The nanoparticle method is as follows:

[0039] (1) Place the one-mouth bottle containing 2ml of SEV solution on a magnetic stirrer, then add 25ul of the above-mentioned ICG dropwise under stirring conditions, and continue stirring after the dropwise addition;

[0040] (2) Configure CaCl with a concentration of 200mM 2 Solution, take 125ul and add dropwise to the above reaction system, and continue to stir for 15min after the dropwise addition;

[0041] (3) Configure Na with a concentration of 200mM 2 HPO 4 solution, and add 25ul dropwise to the reaction system, and continue to stir for 10min after the dropwise addition;

[0042] (4) After stirring, centrifuge at 10000rpm for 3min, then wash with 1ml double distilled water, continue...

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Abstract

The invention discloses a synthetic method of a mineralized sendai virus-based tumor combined treatment nano preparation, the method is used for synthesizing the photo-thermal tumor combined treatmentnano preparation through bionic simulation of a strategy of mineralizing sendai virus and ICG ((ICG + SEV) coated CaHPO4) by CaHPO4. The method comprises the following specific steps: 1) preparing anindocyanine green aqueous solution; and 2) synthesizing (ICG + SEV) coated CaHPO4 nano particles by a one-pot method. The sendai virus (SEV) can activate the immune function of the body in a whole body mode, and all-around killing and removing of tumors are achieved. The ICG has high photo-thermal conversion efficiency under near-infrared laser irradiation, and tumor photo-thermal treatment can be efficiently guided. The pro-apoptotic protease which promote each other and cause and cause each other exist in a pathway formed by calcium ion signals and tumor cell apoptosis, so that tumor cell apoptosis is accelerated. The composite nanoparticle integrating virus immunotherapy and photothermal therapy can improve the tumor treatment effect.

Description

technical field [0001] The invention belongs to the field of preparation of nano preparations, in particular to a method for synthesizing nano preparations for combined treatment of tumors based on mineralized Sendai virus, CaHPO 4 Strategies for Mineralization of Sendai Virus and ICG Biomimetic Synthesis of Nanoformulations for Combination Photothermal Tumor Therapy. Background technique [0002] At present, tumor is one of the most threatening diseases to human beings in the world. Immunotherapy can treat cancer by activating the human immune system and relying on its own immune function to kill cancer cells and tumor tissues. Therefore, immunotherapy is one of the most effective ways to treat tumors. In view of the fact that the inactivated Sendai virus (SEV) can be used as a foreign body in the body, it can stimulate the systemic immune system to produce a cytokine storm (such as interferon, tumor necrosis factor and various interleukins, etc.), and it has an important...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/768A61K41/00A61K9/51A61K47/02A61P35/00
CPCA61K35/768A61K41/0052A61K9/5115A61P35/00A61K2300/00
Inventor 王涛徐亚楠郑斌王志云黄梦倩
Owner TIANJIN UNIV
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