Synthetic method of mineralized sendai virus-based tumor combined treatment nano preparation
A technology of combined therapy and nano-preparation, applied in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of cumbersome process, harsh environmental requirements, pollution, etc. significant effect
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Embodiment 1
[0023] 1) Accurately weigh 5 mg of indocyanine green (ICG) powder, add 1 mL of ultrapure water, and then ultrasonically dissolve to obtain an aqueous solution of ICG with a concentration of 5 mg / ml.
[0024] 2) Synthesis of (ICG+SEV)@CaHPO 4 The nanoparticle method is as follows:
[0025] (1) Place the single-mouth bottle containing 500ml of SEV solution on a magnetic stirrer, then add 25ul of the above-mentioned ICG dropwise under stirring conditions, and continue stirring after the dropwise addition;
[0026] (2) Configure CaCl with a concentration of 50mM 2 Solution, take 125ul and add dropwise to the above reaction system, and continue to stir for 15min after the dropwise addition;
[0027] (3) Configure Na with a concentration of 50mM 2 HPO 4 solution, and add 25ul dropwise to the reaction system, and continue to stir for 10min after the dropwise addition;
[0028] (4) After stirring, centrifuge at 10000rpm for 3min, then wash with 1ml double distilled water, continu...
Embodiment 2
[0030] 1) Accurately weigh 10 mg of indocyanine green (ICG) powder, add 1 mL of ultrapure water, and ultrasonically dissolve to obtain an aqueous solution of ICG with a concentration of 10 mg / ml.
[0031] 2) Synthesis of (ICG+SEV)@CaHPO 4 The nanoparticle method is as follows:
[0032] (1) Place the one-mouth bottle containing 1ml of SEV solution on a magnetic stirrer, then add 25ul of the above-mentioned ICG dropwise under stirring conditions, and continue stirring after the dropwise addition;
[0033] (2) Configure CaCl with a concentration of 100mM 2 Solution, take 125ul and add dropwise to the above reaction system, and continue to stir for 15min after the dropwise addition;
[0034] (3) Configure Na with a concentration of 100mM 2 HPO 4 solution, and add 25ul dropwise to the reaction system, and continue to stir for 10min after the dropwise addition;
[0035] (4) After stirring, centrifuge at 10000rpm for 3min, then wash with 1ml double distilled water, continue cent...
Embodiment 3
[0037] 1) Accurately weigh 20 mg of indocyanine green (ICG) powder, add 1 mL of ultrapure water, and then ultrasonically dissolve to obtain an aqueous solution of ICG with a concentration of 20 mg / ml.
[0038] 2) Synthesis of (ICG+SEV)@CaHPO 4 The nanoparticle method is as follows:
[0039] (1) Place the one-mouth bottle containing 2ml of SEV solution on a magnetic stirrer, then add 25ul of the above-mentioned ICG dropwise under stirring conditions, and continue stirring after the dropwise addition;
[0040] (2) Configure CaCl with a concentration of 200mM 2 Solution, take 125ul and add dropwise to the above reaction system, and continue to stir for 15min after the dropwise addition;
[0041] (3) Configure Na with a concentration of 200mM 2 HPO 4 solution, and add 25ul dropwise to the reaction system, and continue to stir for 10min after the dropwise addition;
[0042] (4) After stirring, centrifuge at 10000rpm for 3min, then wash with 1ml double distilled water, continue...
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