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Three-dimensional bubble graphene-PEGDA-GelMA photocuring biological material for loading cells, and preparation method and application thereof

A biomaterial and light-curing technology, applied in biochemical equipment and methods, immobilized enzymes, immobilized on or in inorganic carriers, etc., can solve 3DGF structure fragility, insufficient biocompatibility, insufficient mechanical strength, etc. problem, to achieve good biocompatibility, good electrical conductivity, high mechanical strength

Active Publication Date: 2020-02-25
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, 3DGF still has certain limitations, such as insufficient biocompatibility (graphene surface is hydrophobic); 3DGF structure is fragile and insufficient in mechanical strength; compared with traditional biomaterials (such as PEGDA and GelMA), the density of cells that can be loaded is low.

Method used

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  • Three-dimensional bubble graphene-PEGDA-GelMA photocuring biological material for loading cells, and preparation method and application thereof
  • Three-dimensional bubble graphene-PEGDA-GelMA photocuring biological material for loading cells, and preparation method and application thereof
  • Three-dimensional bubble graphene-PEGDA-GelMA photocuring biological material for loading cells, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] In this example, taking 10% PEGDA mixed with 10% GelMA loaded mouse microglial cell line BV2 cells as an example, the medium uses DMEM high glucose + 10% FBS cell culture medium to configure and prepare three-dimensional vesicles loaded with cells Graphene-PEGDA-GelMA photocurable biomaterials.

[0069] Its design and preparation process is as follows figure 1 As shown, it mainly includes designing the final printing shape parameters first, including designing the shape of graphene CVD template, designing the hardness of hydrogel printing, preparing cells and culture medium, and printing shape involving hydrogel. By preparing a metal template according to the shape of the graphene CVD template, a graphene material (that is, three-dimensional bubble graphene) is prepared by chemical vapor deposition (CVD) or chemical corrosion; according to the designed hydrogel printing hardness, the configuration contains PEGDA and The mixture of GelMA, and then the mixture of PEGDA a...

Embodiment 2

[0076] In this example, 10% PEGDA mixed with 10% GelMA loaded human umbilical vein endothelial cells HUVEC cells is taken as an example, and the medium uses EGM2 medium to configure and prepare a three-dimensional cell-loaded graphene-PEGDA-GelMA light-cured biomaterial , whose design and preparation process is as follows figure 1 shown.

[0077] Its preparation method is as follows:

[0078] (1) Take out the sterilized and sterile PEGDA and GelMA stored in the refrigerator in advance, and let it stand at room temperature until the PEGDA is completely melted into a liquid; configure cell culture medium (EGM2 medium, filter-sterilized); weigh 0.3g LAP , 15g PEGDA-700 and 10g GelMA, mixed in 100mL of cell culture medium (hydrogel medium mixture) in a sterile environment, heated in a metal bath at 37°C to melt and mix the GelMA evenly to obtain a hydrogel medium mixture , and stored at 37°C.

[0079] (2) To prepare HUVEC cells for 3D printing, add the suspended cells to the hy...

Embodiment 3

[0083] In this example, 10% PEGDA mixed with 10% GelMA loaded human cervical cancer cell line HeLa cells is taken as an example, and the medium uses DMEM+10% FBS cell culture medium to configure and prepare three-dimensional vesicular graphene-PEGDA loaded with cells -GelMA photocurable biomaterial, its design and preparation process is as follows figure 1 shown.

[0084] Its preparation method is as follows:

[0085] (1) Take out the sterilized and preserved sterile PEGDA and GelMA from the refrigerator in advance, and let it stand at room temperature until the PEGDA is completely melted into a liquid; configure cell culture medium (DMEM+10% FBS, sterilized by filtration); weigh 0.5 g LAP, 10 g PEGDA-700, and 15 g GelMA were mixed in 100 mL of cell culture medium (hydrogel medium mixture) in a sterile environment, heated in a 37°C water bath to melt and mix the GelMA evenly, and stored at 37°C.

[0086] (2) To prepare HeLa cells for 3D printing, add the suspended cells to t...

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Abstract

The invention provides a three-dimensional bubble graphene-PEGDA-GelMA photocuring biological material for loading cells, and a preparation method and an application thereof. The preparation method comprises the following steps that PEGDA, GelMA and a photoinitiator are added into a cell culture medium and mixed uniformly; the suspended cells are added to the resulting mixed liquor to yield a cell-containing hydrogel media mixed liquor; a sample tank is mounted, a water bath heating system of 3D photocuring printing equipment is started, the sample tank is heated, three-dimensional bubble graphene is added into the sample tank and fixed, and the cell-containing hydrogel culture medium mixed liquor is added into the sample tank to obtain a printing mixed liquor; the working parameters of the 3D photocuring printing equipment are adjusted, a printing program is executed, photocuring printing is completed, photocuring biological material is obtained, the material has good biocompatibilityand conductivity, the structure is stable, the mechanical strength is high, and the cell loading amount is high.

Description

technical field [0001] The invention belongs to the field of biological materials, and relates to a cell-loaded three-dimensional bubble-like graphene-PEGDA-GelMA light-cured biological material, a preparation method and an application. Background technique [0002] Hydrogels with specific shapes and structures are prepared by ultraviolet light / visible light (blue light) light curing. During the curing process, culture medium and living cells can be mixed in the material solution to realize the curing and molding of cells and hydrogel matrix. By changing the cured material, curing light intensity, and curing light time, the mechanical properties (hardness) of the cured material can be changed to a certain extent. [0003] Single-layer / multi-layer graphene is prepared on the surface of metal template materials (copper, nickel, etc.) Cell-attached culture substrate. [0004] The biomaterials commonly used in photocuring now mainly include: GelMa and PEGDA. [0005] Polyethy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B29C64/124B29C64/30B33Y10/00B33Y30/00C12N11/089C12N11/08C12N11/14
CPCB33Y10/00B33Y30/00B29C64/124B29C64/30C12N11/08C12N11/14
Inventor 王元华
Owner SHANGHAI UNIV
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