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Method for quickly detecting histamine content in fruit wine

A technology for medium histamine and fruit wine, applied in the field of analysis and detection, can solve the problems of complex processing process, expensive instrument consumables, low detection sensitivity, etc., and achieve the effect of simple operation, reasonable parameter design, and high practicability

Pending Publication Date: 2020-02-28
常州磐诺仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, there is a high-sensitivity detector behind the column, so as to realize the continuous analysis of the sample, which has good specificity and sensitivity, but the pre-treatment process is complicated and the instrument consumables are very expensive
[0006] Capillary electrophoresis chromatography combines the high selectivity of high performance liquid chromatography and the high efficiency of capillary electrophoresis for the detection of histamine. The separation speed is fast and there is no environmental pollution, but the detection sensitivity is low

Method used

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  • Method for quickly detecting histamine content in fruit wine
  • Method for quickly detecting histamine content in fruit wine
  • Method for quickly detecting histamine content in fruit wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Take histamine solution, dansyl chloride solution, and histamine-dansyl chloride derivatives respectively, and use a fluorescence spectrometer to detect the fluorescence spectra of the three substances (attached figure 1 shown).

[0049] Conclusion: Observing the fluorescence spectrum, it can be seen that compared with histamine solution and dansyl chloride solution, histamine-dansyl chloride derivatives have stronger fluorescence, and the maximum emission wavelength is 521nm.

Embodiment 2

[0051] To prepare materials, take dansyl chloride and dissolve it in acetone to prepare a dansyl chloride solution, the concentration of the dansyl chloride solution is 1 mg / mL; then take 1 mL of fruit wine, add 2 mol / L NaOH solution, stir, and then add saturated NaHCO3 solution, adjust the pH to 7.5.

[0052] Then add dansyl chloride solution to the fruit wine, place it in a water bath at 10°C, and react to generate histamine-dansyl chloride derivatives, and the heating reaction time is 10 minutes; then add 0.1mL ammonia water to the fruit wine to stop the reaction and remove the residual dansyl chloride Sulfonyl chloride is filtered with a 0.45 μm organic membrane to remove impurities in the fruit wine; the treated fruit wine is placed under a fluorescence spectrometer for fluorescence detection, and the fluorescence intensity is measured.

[0053] In embodiment 2, fruit wine is peach wine.

Embodiment 3

[0055] When preparing the dansyl chloride solution, prepare the concentrations of 2mg / mL, 3mg / mL, 4mg / mL, 5mg / mL, 6mg / mL, 7mg / mL... 15mg / mL, and the concentrations are successively separated by 1mg / mL. Group control experiment, all the other step parameters of each group of control experiment are identical with embodiment 2.

[0056] The 15 groups of data that embodiment 2, embodiment 3 obtain are carried out statistics again, measure the fluorescence intensity of histamine-dansyl chloride derivative under different solubility, compare the impact of dansyl chloride concentration on fluorescence intensity (as attached figure 2 shown).

[0057] Conclusion: When the concentration of dansyl chloride solution is 10 mg / mL, the fluorescence intensity of histamine-dansyl chloride derivatives is the highest.

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Abstract

The invention discloses a method for quickly detecting the histamine content in fruit wine. Nowadays, enzyme-linked immunosorbent assay, gas chromatography, high-performance liquid chromatography andcapillary electrophoresis chromatography are usually selected and applied to detect the histamine content in the fruit wine, so that the operation process is complex, the price and the operation difficulty are high, and the detection sensitivity is low. To solve the technical problem, the method for quickly detecting the histamine content in the fruit wine is designed. The histamine content in thefruit wine is detected according to a fluorescence analysis method. Because histamine free of fluorescent color developing groups is not fluorescent, the histamine is pretreated so as to ensure smooth operation of fluorescence analysis; a dansyl chloride solution reacts with reactive hydrogen on the histamine to generate a fluorescent histamine-dansyl chloride derivative, and the fluorescent histamine-dansyl chloride derivative is utilized to detect the histamine content in the fruit wine. The method has high practicality.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for rapidly detecting histamine content in fruit wine. Background technique [0002] Histamine is a monoamine formed from histidine under the catalysis of histamine decarboxylase. In the human body, histamine can carry out a variety of physiological reactions. It is not only one of the important mediators of inflammatory response and immune injury, but also has the effect of lowering blood pressure, and it also has a certain promotion effect on tumor treatment. At present, the commonly used methods for detecting histamine include ELISA, gas chromatography, high performance liquid chromatography, and capillary electrophoresis chromatography. [0003] ELISA is detected by enzyme-substrate reaction, and the enzyme used must produce colored or fluorescent substances that are easy to measure. The amount of the enzyme conjugate is determined by the color chang...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74G01N2030/067
Inventor 潘勇
Owner 常州磐诺仪器有限公司
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