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Application of alcaligenes faecalis and metabolites thereof in preventing and treating grain and oil aspergillus flavus and toxins in storage period

A technology of Alcaligenes faecalis and its metabolites, which is applied in the field of Alcaligenes faecalis to efficiently inhibit the pathogenesis of Aspergillus flavus and the synthesis of toxins, with remarkable antibacterial effect and growth inhibition effect

Inactive Publication Date: 2020-03-13
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still few studies on the prevention and control of Aspergillus flavus and toxins during the storage period.

Method used

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  • Application of alcaligenes faecalis and metabolites thereof in preventing and treating grain and oil aspergillus flavus and toxins in storage period
  • Application of alcaligenes faecalis and metabolites thereof in preventing and treating grain and oil aspergillus flavus and toxins in storage period
  • Application of alcaligenes faecalis and metabolites thereof in preventing and treating grain and oil aspergillus flavus and toxins in storage period

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The screening of embodiment 1 candidate bacterial strain N1-4

[0036] The invention collects soil from the rhizosphere of tea trees in Cheyun Mountain, Xinyang City, Henan Province, and isolates a strain of Alcaligenes faecalis which has high antibacterial effect on Aspergillus flavus from the soil through a microbiological screening method. The candidate strain was numbered N1-4.

[0037] (1) Isolation of candidate strain N1-4

[0038] In the rhizosphere area of ​​tea trees, at a depth of 10 cm, the soil rich in humus was collected, suspended in sterile water, and diluted to 10 -5 and 10 -6 , each take 100 μL of the diluted solution, spread it on the surface of the NA medium, and incubate in the dark at 37°C for 48 hours. The NA medium formula is: (beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, agar 15.0g / L, supplemented with distilled water to 1L). )

[0039] After 48 hours of culture in NA medium, single colonies with obvious morphological differences were pi...

Embodiment 2

[0055] The inhibitory action of embodiment 2 Alcaligenes faecalis N1-4 to Aspergillus flavus

[0056] The Experiment of Inhibiting Mycelia Growth of Aspergillus flavus by Alcaligenes faecalis N1-4

[0057] Method: Af conidia were inoculated in PDB solution, cultured at 200 rpm and 28°C for 48 hours to produce mycelial balls. Place a fresh mycelial pellet in the center of the PDA medium. N1-4 cells (100μL, 10 9 cfu / mL) spread on NA medium. Two petri dishes were placed on the buckle, and the PDA medium inoculated with mycelial balls was on the top. With the NA medium without bacteria, the PDA inoculated with the Af mycelial balls cultured in the opposite buckle was used as a control. All treatments were cultured statically for 3 days in the dark at 28°C. Record the hyphal diameter of each group, and calculate the inhibition rate.

[0058] Inhibition rate (%) = (hyphae diameter of the control group - hyphae diameter of the treatment group) / hyphae diameter of the control gr...

Embodiment 3

[0063] Example 3 The biological control of Alcaligenes faecalis N1-4 to peanut, corn, soybean and rice aspergillus flavus and toxin pollution

[0064] Sample Preparation:

[0065] 1) Weigh 100g of peanuts, corn and rice grains respectively and store them in 250mL conical flasks. Weigh 2 conical flasks for each crop, sterilize at 121°C and 1.01MPa for 20min, and let stand to cool at room temperature;

[0066] 2) Inoculate 1 mL of Af conidia (1×10 5 cfu / ml), mixed thoroughly, and different volumes of sterilized water were added to 2 Erlenmeyer flasks of each crop, and Aqualab Series 3 model TE (Decagon Devices, Pullman,) was used to adjust the water activity (aw) to 0.9 and 0.8.

[0067] 3) Divide the peanuts, corn, soybeans and rice under each water activity into two parts, take one part from each crop, arrange them symmetrically, place them in a petri dish, and carry out the experiment with the NA medium inoculated with N1-4 bacterial solution. For butt culture, the other pa...

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Abstract

The invention discloses Alcaligenes faecalis N1-4. A preservation number of the Alcaligenes faecalis N1-4 is CCTCC M 2019299. The metabolites of the compound comprise dimethyl disulfide (DMDS) and methyl isovalerate (MI). Meanwhile, the invention further discloses an application of the strain N1-4 and metabolite in inhibiting aspergillus flavus and toxins in the storage period. The alcaligenes faecalis N1-4 and volatile metabolites thereof can effectively inhibit aspergillus flavus attack and toxin pollution of high-water-activity peanuts, corns, soybeans, rice and the like as well as grains of four crops in a closed culture environment. Meanwhile, the invention also proves that volatile substances generated by N1-4 have a broad-spectrum antibacterial effect and can efficiently inhibit thegrowth of other six important pathogenic fungi.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a strain of Alcaligenes faecalis, its metabolites and applications thereof. Background technique [0002] Aspergillus flavus is an important soil-borne fungal disease. It can infect a variety of important grains, oil crops and agricultural by-products such as peanuts, corn, rice, soybeans, cottonseeds and nuts in the field and during storage, causing severe It can also produce highly toxic and strong carcinogenic aflatoxin (AFT), which seriously affects human health. So far, 18 kinds of aflatoxins have been found. Among them, AFB1, AFB2, AFG1 and AFG2 are the most common types of crop pollution, especially AFB1 is the most harmful, which can lead to serious liver cancer, kidney cancer and rectal cancer after ingestion. Therefore, the International Agency for Research on Cancer (IARC) classifies it as a class I carcinogen. [0003] The ha...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L3/3571C12R1/05
CPCA23L3/3571C12N1/20C12N1/205C12R2001/05
Inventor 宫安东孔宪巍吴楠楠闫建丽郜振周伶强袁景嘉张静柏廖玉才
Owner XINYANG NORMAL UNIVERSITY
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