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Application of equisetin compound in resisting tobacco brown spot and extraction method of equisetin compound

A technology of tobacco red spot disease and iquamycin, which is applied in the field of pesticide research, can solve the problems of less research and application, and achieve the effect of great application potential

Active Publication Date: 2020-03-17
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine fungi have great potential as biopesticides. However, most of the current research on marine organisms, including marine fungal medicinal resources, is concentrated in the field of medicine, and there is little research and application on them as pesticides.

Method used

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  • Application of equisetin compound in resisting tobacco brown spot and extraction method of equisetin compound
  • Application of equisetin compound in resisting tobacco brown spot and extraction method of equisetin compound
  • Application of equisetin compound in resisting tobacco brown spot and extraction method of equisetin compound

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Embodiment 1

[0038] The marine fungus F.equiseti, preservation number CGMCC 14348, is derived from the strains of coastal tidal plants in Qingdao. Potato dextrose agar medium is used for culture: it contains potato 6g / L, glucose 20g / L, agar 20g / L, and the rest is water; The fungal strains were plated at 28°C for 7 days.

[0039] (2) Fermentation culture of the marine fungus F. equiseti using rice solid medium, adding 40g of rice and 30mL of water to each 500mL Erlenmeyer flask, fermenting 3 bottles, and culturing the fungal strain at 28°C for 30 days.

[0040] (3) The obtained fermented product is soaked once with ethyl acetate, 50L, then soaked once each with methanol-dichloromethane=1:1 and pure methanol, 50L, after concentrating under reduced pressure, the remaining water phase (containing water) was extracted with ethyl acetate, and the product after evaporating ethyl acetate to dryness was dissolved in 90% methanol-water, and extracted with petroleum ether, and the extracted 90% metha...

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Abstract

The invention provides an application of an equisetin compound in resisting tobacco brown spot and an extraction method of the equisetin compound. The invention belongs to the technical field of pesticide research; the equisetin compound can inhibit spore germination of alternaria alternata under the condition of low concentration, carbendazim does not have the effect under the same concentration,and the equisetin compound has quite great application potential.

Description

technical field [0001] The invention belongs to the technical field of pesticide research, and in particular relates to the application and extraction method of izamicin compounds in resisting tobacco red spot disease. Background technique [0002] Red spot disease is an important disease of tobacco, which causes huge economic losses every year. Tobacco red spot disease occurs seriously and expands rapidly in the mature stage. At present, chemical control is mainly used in production, and the negative effects such as pesticide residues, environmental pollution, and pathogen resistance are becoming increasingly apparent. It is urgent to find alternative agents for tobacco red spot disease. Control provides environmentally friendly plant protection products. With the continuous expansion of tobacco planting area, the increase of continuous cropping tobacco fields, the extension of continuous cropping years, the changes of farming system and tobacco varieties, the types of tob...

Claims

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Application Information

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IPC IPC(8): A01N43/36C07D207/36A01P3/00
CPCA01N43/36C07D207/36
Inventor 赵栋霖李义强王鹏胡希好张成省王世建张鹏丁志勇袁源王耀斌徐宗昌郑艳芬
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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