Matching reagent for glycosylated hemoglobin high-pressure liquid chromatography analyzer

Abstract

The invention discloses a matching reagent for a glycosylated hemoglobin high-pressure liquid chromatography analyzer. The matching reagent comprises a reagent A, a reagent B, a reagent C, a reagent Hand a chromatographic column, wherein the reagent A, the reagent B and the reagent C are eluents with different gradient concentrations; the reagent H is a hemolytic agent; the reagent A, the reagentB and the reagent C respectively comprise sodium citrate, citric acid, sodium chloride and a preservative; and the reagent H comprises sodium dihydrogen phosphate, disodium hydrogen phosphate, a surfactant and a preservative. The matching reagent consists of the reagent A, the reagent B, the reagent C, the reagent H and the chromatographic column, and the packing of the chromatographic column ishigh-molecular polymer ion exchange agent; and the matching reagent can carry out no treatment and pretreatment on a sample, can realize direct original pipe mounting, can separate HbF, L-HbA1c and HbA1c by matching with a instrument, and is good in correlation by comparing the detection results with the detection results of imported instrument and the matching reagent.

Description

technical field [0001] The invention belongs to the technical field of liquid phase chromatography reagents, and in particular relates to a matching reagent for a glycated hemoglobin high pressure liquid phase chromatography analyzer. Background technique [0002] L-HbA1c is the abbreviation of labile hemoglobin A1C, which is an aldimine compound formed by the amino reaction between glucose and the valine residue at the N-terminal of the β chain of hemoglobin HbA, and also forms S-HbA1c ( stable hemoglobin A1C) stabilizes the intermediate product of glycated hemoglobin. S-HbA1c is what we usually call glycosylated hemoglobin, which is the gold standard for diabetes diagnosis and treatment monitoring and evaluation. [0003] HbF (foetal haemoglobin) is commonly known as fetal hemoglobin. In normal adults, HbF only accounts for 0.5% of total hemoglobin. However, certain conditions such as thalassemia and hereditary HbF persistent hyperplasia can cause high HbF. [0004] Howe...

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Problems solved by technology

[0003] HbF (foetal haemoglobin) is commonly known as fetal hemoglobin, normal adult HbF only accounts for 0.5% of total hemoglobin, but some conditions such as thalassemia, hereditary HbF persistent hyperplasia, etc. will cause high HbF

[0004] However, the existing low-pressure liquid chromatography cannot completely separate HbF, L-HbA1c, and HbA1c. When some clinical samples have abnormal HbF or L-HbA1c, it will cause a false increase in the measured value of HbA1c, resulting in inaccurate test results. precise

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