Phase Separation Behavior Modifiers for Two-Aqueous Phase Separation in Porous Materials
A porous material and phase separation technology, applied in separation methods, analytical materials, liquid separation, etc., can solve problems such as yield limitation, ATPS performance is not very stable, affect the purification efficiency, purity and yield of target analytes, and achieve Effects of increasing productivity and improving stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0235] Reality Example 1 - Use of dextran to reduce body mass in a device used to detect pathogens (Streptococcus mutans) in patient samples Product ratio fluctuations.
[0236] Preparation of sample pads for LFA: Glass fiber porous paper was cut into 0.5 cm x 4 cm rectangles. The prepared ATPS fraction, 20% (w / w) PVP and 18.5% (w / w) sodium cholate were pipetted onto glass fiber porous paper. The above porous paper with ATPS was then dried in a lyophilizer for 2 hours. 10% (w / w) dextran was then pipetted onto glass fiber porous paper as a modifier of phase separation behavior on the paper segment. PEG solution (in DI H 2 0) was added to each porous material. 50 μl of a Tris-buffer solution (20 mM Tris, pH 7.5) containing 2% bovine serum albumin (BSA) and 0.1% PEG was added immediately after the first solution.
[0237] The above porous paper containing ATPS and phase separation behavior modifier was then dried in a lyophilizer for 2 hours. The ATPS in porous paper was...
example 2
[0245] Example 2 - Use of polysorbate and sucrose to increase partitioning of food allergen (lupine) in detection devices
[0246] Preparation of sample pads for LFA: Glass fiber porous paper was cut into 0.5 cm x 4 cm rectangles. The prepared ATPS fraction, 20% (w / w) PVP and 18.5% (w / w) sodium cholate were pipetted onto glass fiber porous paper. 5% (w / w) polysorbate and 5% (w / w) sucrose were pipetted onto porous glass fiber paper as phase separation behavior modifiers. PEG solution (in DI H20) was added to each porous material. 50 μl of Tris-buffered solution (20 mM Tris, pH 7.5) containing 2% bovine serum albumin (BSA) and 0.1% PEG was added immediately after the first solution.
[0247] The above porous paper containing the dehydrated ATPS component and phase separation behavior modifier was then dried in a lyophilizer for 1 hour. The ATPS dehydrated porous paper was then placed in a buffer solution containing the indicator (colloidal gold) in PBS (total pH 7.4) to ind...
example 3
[0255] Example 3 - Use of Potassium Phosphate to Increase Total Phase Separation Volume in Sample Solution Purification and DNA Amplification Devices
[0256] The porous hydrogel material was cut into cylindrical shapes with a radius of 2 cm and a height of 6 cm. The porous hydrogel components are integrated into suitable boxes / housings. The device can expose the bottom and top of the hydrogel.
[0257] The ATPS component, 20% (w / w) PVP and 18.5% (w / w) sodium cholate, and 10% (w / w) potassium phosphate as a phase separation behavior modifier were mixed with the blood sample solution, the resulting The blood sample solution contained the relevant DNA fragments in pH 7.4 PBS buffer solution. The purpose of potassium phosphate is to induce macroscopic phase separation at an earlier point (further upstream) during the flow of the solution through the porous material, allowing a larger volume of solution to undergo phase separation and enabling a larger volume to be collected at...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com