Scar/adhesion barrier film and preparation method and application thereof

A technology for blocking membranes and scars, applied in medical tissue engineering and regenerative medicine, medical biomaterials, and medical fields. It can solve problems such as secondary surgery difficulties, patient pain, and adhesions, and achieve good blood adsorption performance and strong coagulation. , good adhesion effect

Active Publication Date: 2020-03-20
许和平 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This natural mechanism in the body will lead to severe adhesion of postoperative viscera and its surrounding tissues on the surface of the organ and its surroundings, resulting in complications, resulting in difficulties in secondary operations and pain for patients; recompression of bleeding scars in intraspinal surgery in spinal surgery The recurrence of pain and the failure of the operation are all urgent problems to be solved in the medical field.

Method used

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  • Scar/adhesion barrier film and preparation method and application thereof
  • Scar/adhesion barrier film and preparation method and application thereof
  • Scar/adhesion barrier film and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Extraction of type I collagen materials that maintain the unique triple helical structure of collagen

[0031] According to the method recorded in CN101569765B, the beef Achilles tendon imported from China and Australia that passed the quarantine inspection was selected to prepare type I collagen material. After removing impurities such as tendon sheath and surrounding adipose tissue, and then cutting-degreasing-alkalization-neutralization-cleaning process, the natural type I collagen material with high purity and triple helix structure is obtained.

[0032] Take bovine Achilles tendon tissue as raw material, freeze it, cut it into thin slices with a thickness of 0.1-3 mm, soak it in 0.1% sodium dihydrogen phosphate / sodium hydroxide solution for 1-3 hours under stirring conditions, and make it pH Keep neutral; add 1:5 (w / w) fig enzyme to the above-mentioned treated substance solution to fully react; stir regularly for 4 hours, then treat with 1% ammonium nitr...

Embodiment 2

[0034] Embodiment 2: Preparation of scar / adhesion barrier film

[0035] The type I collagen material obtained in Example 1 was dissolved in 720ml of 0.1M glacial acetic acid, configured into a 0.5wt% suspension, stirred at an initial speed of 1000 rpm at a temperature of 5°C for 20 minutes, and then The rotation speed was increased to 20,000 rpm and stirring was continued for 4 hours. The stirred suspension was placed in a 30 x 150 cm mold and freeze-dried.

[0036] During the freeze-drying process, the pre-freezing temperature was set at -10°C for 45 minutes, which was in a continuous state; the freezing temperature was set at -50°C, and the time was 150 minutes, which was in a dynamic state; the temperature of the freezer in the freeze dryer was maintained at -80°C . Thereafter, the temperature in the sublimation stage was set at 0° C. for 24 hours; the temperature for the secondary drying was set at 20° C. for 45 minutes. The vacuum was set at 200 mTorr during the freeze...

Embodiment 3

[0040] Embodiment 3: Preparation of scar / adhesion barrier film

[0041] The type I collagen material obtained in Example 1 was dissolved in 720ml of 0.8M glacial acetic acid, configured as a 2wt% suspension, and stirred at an initial speed of 1500 rpm for 10 minutes at a temperature of 15°C, and then the speed was increased to Increase to 25,000 rpm and continue stirring for 3 hours. The stirred suspension was placed in a 30 x 150 cm mold and freeze-dried.

[0042] During the freeze-drying process, the pre-freezing temperature is set at -5°C for 30 minutes, which is in a continuous state; the freezing temperature is set at -40°C, and the time is 120 minutes, which is in a dynamic state; the temperature of the freezer of the freeze dryer is maintained at -80°C . Thereafter, the temperature in the sublimation stage was set at 0° C. for 18 hours; the temperature for the secondary drying was set at 30° C. for 30 minutes. The vacuum was set at 300 mTorr during the freeze-drying ...

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Abstract

The invention relates to a scar / adhesion barrier film prepared from single type-I collagen keeping a triple-helix structure, and a preparation method and application of the scar / adhesion barrier film.The barrier film has the characteristics of low immune antigenicity, good biocompatibility, high platelet aggregation function, intramembrane coagulation, controllable degradation rate of biologicalmaterials, certain thickness and flexible application performance, and particularly, the prepared barrier film has the characteristic of scar barrier effect, and is suitable for preventing or relieving hemorrhagic adhesion complications caused by any visceral trauma and postoperative bleeding.

Description

technical field [0001] The invention relates to the technical fields of medicine, medical biomaterials, medical tissue engineering and regenerative medicine, in particular to a scar / adhesion barrier film prepared from a single type I collagen maintaining a triple helix structure, its preparation method and application. Background technique [0002] At the end of the 20th century, with the rapid development of holistic medicine, cell biology, molecular biology, genetics, tissue engineering, and regenerative medicine also continued to progress and develop. Biomaterials with good properties are also being rapidly discovered and utilized. The rapid development of tissue engineering and regenerative medicine in clinical medicine in recent years proves this point. Tissue engineering and regenerative medicine products using natural insoluble type I collagen as a carrier or repair scaffold have achieved remarkable development and application. [0003] How to discover and extract s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/04A61L31/14
CPCA61L31/044A61L31/148A61L31/146A61L31/14A61L2400/04
Inventor 许和平马克·斯拜克迈伦·斯佩克特
Owner 许和平
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