Method for separating maternal mesenchymal stem cells from human placenta decidua basalis tissues

A technology for placental decidua and stromal cells, which is applied in the field of cell biology, can solve the problems of high production cost, insufficient safety, and non-single cell components, so as to reduce material cost and time cost, shorten culture period, and improve separation. The effect of efficiency

Inactive Publication Date: 2020-03-24
FUJIAN YINFENG STEM CELL ENG +1
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the separation and preparation process of placental decidua mesenchymal stem cells is complicated; more importantly, most of the current techniques have not identified the source of the isolated PDB-MSCs, and there is a problem of uncertain sample sources
[0005] In prior art methods, enzymatic digestion method is mostly used to separate PDB-MSCs, so as to obtain a cell group of PDB-MSCs with higher purity as much as possible;

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating maternal mesenchymal stem cells from human placenta decidua basalis tissues
  • Method for separating maternal mesenchymal stem cells from human placenta decidua basalis tissues
  • Method for separating maternal mesenchymal stem cells from human placenta decidua basalis tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for isolating maternal-derived mesenchymal stem cells from human placental decidual base tissue, comprising the following specific steps:

[0043] 1. Preparation of placental decidua mesenchymal stem cells:

[0044] 1) Sample screening and collection: select customers who are qualified in all infectious disease tests, and collect 5ml of placental tissue and maternal blood samples after caesarean section; the placenta is rinsed with normal saline, placed in a sterile collection bag, and poured into 500ml of storage and transportation solution. Seal, then put the sterile collection bag into a biosafety sample bag, place it in a storage and transportation box, and transport it to the laboratory at 4-10°C; the formula of the placenta storage and transportation solution is: 500ml PBS+ 320U / ml sulfuric acid Gentamicin + 25 μg / ml amphotericin B.

[0045] 2) Preliminary sample inspection: After arriving at the laboratory, receive the placenta sample and maternal blood...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for separating maternal mesenchymal stem cells from human placenta decidua basalis tissue, which belongs to the technical field of cell biology. The method comprises the following steps: separating maternal mesenchymal stem cells from human placenta decidua basalis tissues, and carrying out subculture, cell harvesting, cryopreservation and cell STR identification to finally obtain a cell product. In the separation and culture process, an improved separation method and an improved culture system are adopted to reduce the damage to cells, improve the separation efficiency, shorten the culture period and obtain high-purity cells with excellent characterization.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to a method for isolating maternal-derived mesenchymal stem cells from human placental decidua tissue. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) are a kind of adult stem cells with multi-directional differentiation potential and low immunogenicity. They are adhesive, spindle-shaped, and fibroblast-like in shape. The germ layer, which has the potential to differentiate into a variety of mesoderm cell lines, including bone cells, adipocytes, chondrocytes, fibroblasts, tenocytes, etc., has great potential medical and storage value. MSCs widely exist in human bone marrow, fat, periodontal ligament, neonatal umbilical cord, placenta, amniotic membrane, amniotic fluid and other tissues, and they still maintain multi-directional differentiation potential after multiple divisions and passages. It is found that MSCs have serious sid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/073C12N5/0775
CPCC12N5/0605C12N5/0668C12N2500/32C12N2501/11C12N2501/115C12N2501/135C12N2501/24C12N2501/25C12N2509/00
Inventor 苏爱盟肖桂清徐峰波唐燕程谢燕萍
Owner FUJIAN YINFENG STEM CELL ENG
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap