Method for determining trace hydrogen peroxide based on tetracarboxyl nickel phthalocyanine

Abstract

The invention discloses a method for determining trace hydrogen peroxide based on tetracarboxyl nickel phthalocyanine. The method comprises the following steps of in seven colorimetric tubes, respectively adding an HAc-NaAc buffer solution, a NiC4Pc solution and an OPD solution and sequentially adding 0.0 mL of a H2O2 solution with the concentration of 1.97 * 10 <-5 > mol / L, 0.0017 mL of a H2O2 solution with the concentration of 0.0017 mol / L, 0.0025 mL of a H2O2 solution with the concentration of 0.005 mol / L, 0.01 mL of a H2O2 solution with the concentration of 0.02 mL and 0.04 mL of a H2O2 solution with the concentration of 0.04 * 10 <-5 > mol / L; reacting in a water bath at 25 DEG C for 15 minutes, adding a nanogold solution into each branch pipe, diluting to 5mL by using secondary distilled water after 4 minutes, and scanning a scattering spectrum on a fluorophotometer in a lambda ex = lambda em manner; and respectively measuring the scattering intensity I700nm of the H2O2 solutionand the scattering intensity I0 of the reagent blank without H2O2 at the wavelength of 700nm, and calculating the scattering intensity difference delta I700nm = I700nm-I0. The method is good in selectivity and high in sensitivity.

Description

technical field [0001] The invention relates to a method for determining trace amounts of hydrogen peroxide, in particular to a resonance scattering spectrometry method for determining hydrogen peroxide based on tetracarboxy nickel phthalocyanine. Background technique [0002] Hydrogen peroxide is a common disinfectant and bleaching agent, often used in industries such as food, pharmaceuticals, clinical medicine, organic synthesis and environmental analysis. But at the same time, hydrogen peroxide is also harmful. Long-term exposure can cause respiratory and skin diseases, accelerate the aging of the human body, and induce gene mutations. Therefore, it is very important to establish a simple, rapid and economical monitoring method for hydrogen peroxide. Methods commonly used to detect hydrogen peroxide include: titration analysis, spectrophotometry, liquid chromatography, electrochemical method, fluorescence photometry, resonance scattering spectroscopy, etc. Resonance sca...

AI Technical Summary

Problems solved by technology

Tetracarboxy nickel phthalocyanine (NiC 4 Pc) has the properties of hydrogen peroxide mimetic enzyme, which can catalyze the oxidation of o-phenylenediamine (OPD) by hydrogen peroxide in weakly acidic solution to generate intermediate products 2,3-diamino-5,10-dihydrophenazine and The final product is 2,3-diaminophenazine. Both products can induce the aggregation of gold nanoparticles, resulting in the enhancement of the resonance scattering signal of the reaction system. According to the change of the resonance scattering intensity of the system before and after the reaction, a quantitative method for the determination of hydrogen peroxide can be established. This method has not yet been reported

Images