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Method for determining fluorouracil content in blood plasma by HPLC method

A technology for fluorouracil and mesofluorouracil is applied in the field of determination of fluorouracil content in plasma by HPLC, which can solve the problems of bone marrow transplantation, irregular absorption and metabolism, and complexity, and achieves the effects of good repeatability, high sensitivity and accurate content determination.

Pending Publication Date: 2020-03-24
HEILONGJIANG FUHE HUAXING PHARMA GROUP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Fluorouracil has a short metabolic half-life in the body, high toxicity, irregular absorption and metabolism, and excessive concentration in the body is prone to adverse reactions such as bone marrow transplantation
And the composition of plasma is extremely complex, including protein, lipid, inorganic salt, sugar, amino acid, metabolic waste and a large amount of water; how to detect the drug concentration in plasma is the basis of pharmacokinetic research; therefore, in order to detect fluorouracil in plasma The prior art has established a series of determination methods for the content of fluorouracil in plasma. For example, Ye Lika et al. disclosed a method for determining the concentration of 5-FU in human plasma in the journal document "High Performance Liquid Chromatography Determination of 5-FU Concentration in Human Plasma". In the method of fluorouracil content, the method firstly prepares the reference substance solution, and then processes the plasma. During the processing, ethyl acetate is used as the extraction agent, and then the test solution is obtained through vortex, centrifugation and other operations, and finally the HPLC method is used to obtain the test solution. For content determination, due to the poor solubility of fluorouracil, the recovery rate of this content determination method is low, and the precision is poor

Method used

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  • Method for determining fluorouracil content in blood plasma by HPLC method
  • Method for determining fluorouracil content in blood plasma by HPLC method
  • Method for determining fluorouracil content in blood plasma by HPLC method

Examples

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Embodiment 1

[0033] A kind of HPLC method is measured the method for fluorouracil content in blood plasma, and this method comprises the steps:

[0034] (1) Preparation of reference substance solution

[0035] Dissolve fluorouracil in methanol, dilute with methanol to a solution containing 0.1 mg of fluorouracil per mL of methanol, as a reference solution; draw 1 mL, dilute with mobile phase to contain 0.25 μg of fluorouracil per mL, and the mobile phase is 0.1 mol / L disodium hydrogen phosphate aqueous solution (phosphoric acid adjusts pH to 3.2): acetonitrile=90:10;

[0036] (2) Sample pretreatment

[0037] Take 100 μL of plasma to be tested, put it in a 5 mL test tube, add 1 mL of 0.1 mol / L disodium hydrogen phosphate aqueous solution (phosphoric acid to adjust the pH to 6-7) and shake well, add 0.5 mL of extraction agent, the extraction agent is: the volume ratio is 8:1:1 mixture of ethyl acetate, acetonitrile and triethylamine; use 0.5mol / L disodium hydrogen phosphate aqueous soluti...

Embodiment 2

[0048] A kind of HPLC method is measured the method for fluorouracil content in blood plasma, and this method comprises the steps:

[0049] (1) Preparation of reference substance solution

[0050] Dissolve fluorouracil in methanol, dilute with methanol to a solution containing 0.1 mg of fluorouracil per mL of methanol, as a reference solution; draw 1 mL, dilute with mobile phase to contain 0.25 μg of fluorouracil per mL, and the mobile phase is 0.1 mol / L disodium hydrogen phosphate aqueous solution (phosphoric acid adjusts pH to 3.2): acetonitrile=90:10;

[0051] (2) Sample pretreatment

[0052] Take 100 μL of plasma to be tested, put it in a 5 mL test tube, add 1 mL of 0.1 mol / L disodium hydrogen phosphate aqueous solution (phosphoric acid to adjust the pH to 3.2) and shake well, add 0.5 mL of extraction agent, the extraction agent is: the volume ratio is 8: 1:1 mixture of ethyl acetate, acetonitrile and triethylamine; adjust the pH to 6-7, vortex shake for 3min, centrifug...

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Abstract

The invention provides a method for determining the fluorouracil content in blood plasma by an HPLC (High Performance Liquid Chromatography) method. The method comprises the following steps: preparing a reference substance solution, pretreating a sample and determining the content by a high performance liquid chromatography. The method provided by the invention has the characteristics of accuratecontent determination, high sensitivity and good repeatability.

Description

technical field [0001] The invention belongs to the field of content determination, in particular to a method for determining the content of fluorouracil in blood plasma by HPLC. Background technique [0002] Fluorouracil is a pyrimidine anti-metabolite drug commonly used in clinical practice. It inhibits thymidine nucleotide synthase, blocks the conversion of deoxypyrimidine nucleotides into thymidine, and interferes with DNA synthesis. Fluorouracil is mainly used to treat gastrointestinal tumors, or in larger doses to treat choriocarcinoma, and is also used to treat breast cancer, lung cancer, ovarian cancer, cervical cancer, skin cancer, etc. [0003] The metabolic half-life of fluorouracil in the body is short, the toxicity is high, the absorption and metabolism are irregular, and the concentration in the body is too high to easily cause adverse reactions such as bone marrow transplantation. And the composition of plasma is extremely complex, including protein, lipid, i...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 吴光彦
Owner HEILONGJIANG FUHE HUAXING PHARMA GROUP