Garlic complex microbial inoculant containing multiple microorganisms and preparation method thereof

A compound inoculum and microorganism technology, applied in the field of garlic compound inoculum and its preparation, can solve the problems of deterioration of soil physical and chemical properties, decrease in garlic yield, soil compaction, etc., and achieve reduction of planting cost, enhancement of absorption and utilization, and improvement of mass structure. Effect

Active Publication Date: 2020-04-03
河南新仰韶生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous cropping of garlic and the extensive use of inorganic fertilizers, the problem of soil compaction is becoming more and more serious, the physical and chemical properties of the soil are deteriorating, and the toxic substances metabolized by the garlic root system inhibit the reproduction and activity of beneficial microorganisms in the soil, making the yield of garlic continue to decline. , how to effectively improve the utilization rate of fertilizers, reduce the cost of planting, and increase the yield of garlic has become an urgent problem to be solved in the garlic planting industry

Method used

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  • Garlic complex microbial inoculant containing multiple microorganisms and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0024] The special composite bacteria agent for garlic in the present embodiment contains 300 g of Bacillus subtilis with a vigor of 50 billion / g, 400 g of Bacillus licheniformis with a vigor of 50 billion / g, and megaspores with a vigor of 10 billion / g 200g of bacillus, 95g of Bacillus lateralis with an activity of 5 billion / g, and 5g of Bacillus jelly with an activity of 1 billion / g.

[0025] The above-mentioned production method of the special compound bacterial agent for garlic containing various microorganisms, it may comprise the steps:

[0026] (1) Production of Bacillus subtilis: carry out liquid culture of Bacillus subtilis, then carry out fermentation culture, carry out spray drying after fermentation culture, grind into powder and set aside. The medium composition used for liquid culture is: 40 mesh cornmeal 5g, soybean meal 20g, sodium chloride 1g, ammonium sulfate 1g, pH6.5; liquid culture conditions are: temperature 30°C, shaker speed 150r / min, culture time 18h. ...

Embodiment 2

[0033] A kind of special compound bacterial agent for garlic containing various microorganisms, each 1kg of special compound bacterial agent for garlic contains 400g of Bacillus subtilis with an activity of 60 billion / g, 300g of Bacillus licheniformis with an activity of 30 billion / g, and Bacillus licheniformis with an activity of 20 billion / g 200g of Bacillus megaterium, 80g of Bacillus lateralis with an activity of 10 billion / g, 20g of Bacillus jelly with an activity of 5 billion / g.

[0034] The above-mentioned production method of the special compound bacterial agent for garlic containing various microorganisms, it may comprise the steps:

[0035] (1) Production of Bacillus subtilis: carry out liquid culture of Bacillus subtilis, then carry out fermentation culture, carry out spray drying after fermentation culture, grind into powder and set aside. The composition of the medium used in liquid culture is: 15g of 40-mesh cornmeal, 30g of soybean meal, 1g of sodium chloride, 2...

Embodiment 3

[0042] The special compound bacteria agent for garlic in this embodiment contains 500 g of Bacillus subtilis with vigor of 200 billion / g, 300 g of Bacillus licheniformis with vigor of 200 billion / g, and gigantic spores of vigor with 30 billion / g Bacillus 100g, activity 20 billion / g Bacillus lateralis 70g, activity 10 billion / g Bacillus jelly 30g.

[0043] The above-mentioned production method of the special compound bacterial agent for garlic containing various microorganisms, it may comprise the steps:

[0044] (1) Production of Bacillus subtilis: carry out liquid culture of Bacillus subtilis, then carry out fermentation culture, carry out spray drying after fermentation culture, grind into powder and set aside. The medium composition used for liquid culture is: 100 mesh cornmeal 15g, soybean meal 40g, sodium chloride 5g, ammonium sulfate 5g, pH 8.0; liquid culture conditions are: temperature 40°C, shaker speed 250r / min, culture time 30h. The medium composition used for ferm...

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Abstract

The invention discloses a garlic complex microbial inoculant containing multiple microorganisms and a preparation method of the garlic complex microbial inoculant. Every 1000 g of the garlic complex microbial inoculant is prepared from 200 g to 500 g of bacillus subtilis, 200 g to 400 g of bacillus licheniformis, 50 g to 200 g of bacillus megaterium, 20 g to 100 g of bacillus laterosporus and 5 gto 30 g of bacillus mucilaginosus. The preparation method comprises the following steps: S1, preparing bacillus subtilis powder; S2, preparing bacillus licheniformis powder; S3, preparing bacillus megaterium powder; S4, preparing bacillus laterosporus powder; S5, preparing bacillus mucilaginosus powder; and S6, uniformly mixing the powder in proportion to obtain the garlic composite microbial inoculant. Through the mutual influence and synergistic effect of various microorganisms, the absorption and utilization of the garlic on a fertilizer are enhanced, the use amount of inorganic fertilizeris reduced, the planting yield of the garlic is increased, and the planting cost of the garlic is effectively reduced, so that the economic benefit of the garlic planting industry is increased.

Description

technical field [0001] The invention relates to the field of microorganism preparation, in particular to a garlic compound bacterial agent containing various microorganisms and a preparation method thereof. Background technique [0002] For a long time, in the process of garlic cultivation in our country, the application of inorganic fertilizers and drugs has been relied on as the main means of crop yield increase and disease resistance. With the continuous cropping of garlic and the extensive use of inorganic fertilizers, the problem of soil compaction is becoming more and more serious, the physical and chemical properties of the soil are deteriorating, and the toxic substances metabolized by the garlic root system inhibit the reproduction and activity of beneficial microorganisms in the soil, making the yield of garlic continue to decline. , How to effectively improve the utilization rate of fertilizers, reduce planting costs, and increase garlic yield has become an urgent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125C12R1/11C12R1/10C12R1/07
CPCC12N1/20
Inventor 张永钦焦国宝邹瑞瑞朱飞帆段波陈晓宇郭蒙恩卢佳琦李佳王鑫涛崔红健梁颖杰刘芦鹏
Owner 河南新仰韶生物科技有限公司
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