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Method for detecting nicotinic acid content in peanuts and products thereof

A detection method and product technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex, cumbersome steps, etc., and achieve the effect of simple operation and high reproducibility

Inactive Publication Date: 2020-04-03
HENAN GUANGDIAN METROLOGY & TESTING CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

GB5009.89-2016 National food safety standard Determination of niacin and niacinamide in food, high-performance liquid chromatography for the determination of niacin and niacinamide in food The matrix is ​​not suitable for peanuts and their products, and the detection method of microbial technology, the steps are relatively cumbersome ,complex
[0004] For the detection of niacin in peanuts and its products, the national standard method and the literature mainly use the detection method of microbial technology, and the steps are relatively cumbersome and complicated

Method used

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  • Method for detecting nicotinic acid content in peanuts and products thereof
  • Method for detecting nicotinic acid content in peanuts and products thereof
  • Method for detecting nicotinic acid content in peanuts and products thereof

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Embodiment Construction

[0027] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0028] The technical route and technological process of the specific detection method of this embodiment are as attached figure 1 As shown, the specific detection process is as follows:

[0029] 1) Sample preparation

[0030] The solid sample was fully pulverized with a grinder and stirred evenly, and 200 g of it was put into a sample bottle, sealed, and the sample was stored at 4°C in the dark.

[0031] 2) Pretreatment

[0032] Weigh 10.0g of peanut and its product samples into a 100mL Erlenmeyer flask (with a soft stopper), add 60mL of 0.1mol / L hydrochloric acid solution, shake well, put on a soft stopper, and place in an autoclave at 121°C Keep it for 30min. After hydrolysis, take it out after cooling to below 40°C, shake it several ti...

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Abstract

The invention discloses a method for detecting the content of nicotinic acid in peanuts and products thereof. The method comprises the following steps: (1) pretreating peanut and product samples thereof; and (2) detecting and analyzing the pretreated sample by using a high performance liquid analyzer. Detection method, the adding standard recovery rate of nicotinic acid in peanuts and products thereof reaches 80-120%; rSD (Received Signal Deviation) of precision and stability test within 24 hours is less than 10%; the method is high in reproducibility, the detection limits of nicotinic acid and nicotinamide are 0.4289 mg / kg and 0.2519 mg / kg respectively, the quantification limits of nicotinic acid and nicotinamide are 1.4296 mg / kg and 0.8397 mg / kg respectively, and quantitative analysis ofnicotinic acid in peanuts and products thereof can be met. The adopted pretreatment method is simple, convenient and quick to operate, provides new technical support and data support for national food safety standards, and can provide corresponding matched detection standards for national standards.

Description

technical field [0001] The invention relates to a method for detecting the content of niacin, in particular to a method for detecting the content of niacin in peanuts and products thereof. Background technique [0002] Peanut is one of the main oil crops and economic crops in my country, and has high nutritional value. The main components of peanuts are fat, protein and carbohydrates. Peanuts are rich in vitamins such as vitamin B1, vitamin B2, niacin and vitamin E, minerals such as calcium, iron, potassium and sodium, and essential amino acids for human body. In addition, peanuts also contain functional active ingredients such as flavonoids and phenolic compounds, which are of great significance to human dietary nutrition. Peanuts are known as "longevity fruit". They play an important role in regulating human physiological functions, delaying the decline of brain function, preventing heart disease and arteriosclerosis, especially in reducing blood cholesterol levels, preve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 范玲靳艳军朱晓路侯赛蔺丹华张晓莉
Owner HENAN GUANGDIAN METROLOGY & TESTING CO LTD
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