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Tissue-engineered urethral stent graft, and preparation method and application thereof

A tissue engineering and graft technology, applied in biochemical equipment and methods, tissue regeneration, tissue culture, etc., can solve problems such as high incidence of complications, protracted and difficult recovery of autologous tissue repair, affecting the effect of surgery, urination and reproductive function, etc. , to achieve the effect of repairing urethral damage

Inactive Publication Date: 2020-04-07
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The plastic surgery of hypospadias or traumatic injury has undergone significant progress after more than a hundred years of development, and there are hundreds of methods, but there is still no optimal solution with significant effect, and postoperative urethral fistula, urethral stricture, diverticulum formation and The incidence of complications such as penile curvature is still high, and about 1 / 5 of the patients need to undergo secondary or even multiple surgical repairs, and some patients have scar constitution, and autologous tissue repair is protracted and difficult to heal, resulting in penis and urethral disabilities , have serious adverse effects on the physical and mental development of children
[0004] The mechanical properties, biocompatibility and post-transplant necrosis of the existing urethral graft stents are affected by many factors, which greatly affect the surgical effect and long-term postoperative urination and reproductive function.

Method used

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  • Tissue-engineered urethral stent graft, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for preparing a tissue-engineered urethral stent graft.

[0041] Weigh 0.9g of the first polymer material (L-polylactic acid, molecular weight 30000Da), then weigh 0.1g of the second polymer material (collagen), place in a 15ml glass flask, add organic solvent (tetrahydrofuran) to 10ml , fully dissolved to obtain electrospinning solution.

[0042] Use a pipette to transfer the dissolved electrospinning liquid into the electrospinning syringe, connect the spinning nozzle (inner diameter 22G), set the high voltage to 15kV, the low pressure to -5kV, the flow rate to 3.0mL / h, and rotate the receiving device The distance from the nozzle is 12cm, and the receiving device is a cylindrical stainless steel tube with an outer diameter of 2.0cm and a length of 18cm. The rotational speed of the receiving device was adjusted to 900 rpm, and the tubular nanofibers were collected by electrospinning for 48 hours.

[0043] Harvest the successfully electrospun tubular nanofibe...

Embodiment 2

[0051] This embodiment provides a preparation method of a tissue-engineered urethral stent-graft, which is roughly the same as the preparation method provided in Example 1, except that the components of the tissue-engineered urethral stent-graft are different, and the differences are as follows:

[0052] The first polymer material used is polyglycolic acid (molecular weight 30000Da); the second polymer material is gelatin, and the organic solvent is chloroform.

Embodiment 3

[0054] This embodiment provides a preparation method of a tissue-engineered urethral stent-graft, which is roughly the same as the preparation method provided in Example 1, except that the components of the tissue-engineered urethral stent-graft are different and the preparation method is different.

[0055] The components differ as follows:

[0056] The first polymer material used is polycaprolactone (molecular weight 30000Da); the second polymer material is gelatin, and the organic solvent is dichloromethane.

[0057] The difference in preparation method is as follows:

[0058] Smooth muscle cells were isolated from kidney tissue in children, and after in vitro culture, renal smooth muscle cells were seeded on tubular nanofiber scaffolds.

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Abstract

The invention discloses a tissue-engineered urethral stent graft, and a preparation method and application thereof, belonging to the technical field of tissue engineering. The tissue-engineered urethral stent graft is a macromolecular degradable nanofiber tube inoculated with epithelial cells and smooth muscle cells, wherein the epithelial cells are epithelial cells of epithelial progenitor cellswith monopotent differentiation potential, the degradation rate of the nanofiber tube can be matched with the regeneration rate of urethral repair, and urethral injury can be effectively repaired.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a tissue-engineered urethral stent graft and its preparation method and application. Background technique [0002] Hypospadias, urethral straddle injury or motorcycle defects in infants and young children are the most common diseases of the urinary system. The child is unable to urinate normally and must undergo urethral reconstruction surgery. Among them, the incidence of congenital hypospadias in children is about 3.2‰. At present, autologous tissue transplantation (such as penis and scrotum flap) is mainly used in clinical practice for urethral reconstruction. [0003] The plastic surgery of hypospadias or traumatic injury has undergone significant progress after more than a hundred years of development, and there are hundreds of methods, but there is still no optimal solution with significant effect, and postoperative urethral fistula, urethral stricture, divertic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/22A61L27/24A61L27/38A61L27/50A61L27/58C12N5/071C12N5/077
CPCA61L27/24A61L27/222A61L27/18A61L27/3813A61L27/3826A61L27/50A61L27/58C12N5/0625C12N5/0661C12N2501/11C12N2501/115C12N2501/125C12N2501/33C12N2513/00C12N2533/40C12N2533/30C12N2533/54C12N2533/80A61L2430/22C08L67/04
Inventor 牛玉清夏慧敏付铭张锐忠刘国昌邓富铭
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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