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Production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology

An oat bran, separation and purification technology, applied in membrane technology, semi-permeable membrane separation, ultrafiltration and other directions, can solve the problems of industrial production and large-scale clinical application limitations, increase production costs, and long production cycles, and achieve single High separation efficiency, easy extraction and easy operation

Pending Publication Date: 2020-04-07
植萃优品(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, in order to meet the needs of developing β-glucan, a high-efficiency extraction and separation technology is needed, and the traditional techniques for separating and extracting β-glucan from natural products in the prior art, such as water extraction and alcohol analysis, and thin film concentration method , freeze-drying, etc., all use fruiting bodies as raw materials for production, the process is complicated, and the production cycle is long, which increases the production cost and limits industrial production and large-scale clinical application.

Method used

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  • Production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology
  • Production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology
  • Production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology

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Embodiment Construction

[0029] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0030] A production process for the separation and purification of β-glucan in oat bran based on ultrafiltration membrane technology, oat bran crushing → β-glucan extraction → preparation of β-glucan aqueous solutionmicrofiltration → ultrafiltration → freeze-drying → β-glucan finished product, the specific process steps are as follows:

[0031] (1) Oat bran pulverization: screen bran after raw oat pulverization, mesh 60-120;

[0032] (2) Extraction of β-glucan: Take a buffer solution with pH 4-6 and containing α-amylase, the ratio of bran to liquid is 1:80-100, the extraction temperature is 80-90°C, extract for 5-6 hours, and cool to room temperature , adjusting the pH to 6-8, centrifuging to take the supernatant, alcohol precipitation a...

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Abstract

The invention relates to a production process for separation and purification of beta-glucan in oat bran based on ultrafiltration membrane technology. The process includes: crushing oat bran, extracting beta-glucan, preparing a beta-glucan aqueous solution, carrying out microfiltration, performing ultrafiltration, and conducting freeze drying to obtain a beta-glucan finished product. The innovation point of the process lies in that ultrafiltration technology is utilized to realize efficient extraction, separation and purification, and compared with the traditional separation processes like rectification, extraction, recrystallization, adsorption, etc., the process has the advantages of low energy consumption, high single-stage separation efficiency, simple equipment, no pollution and the like. The process has extremely high beta-glucan separation efficiency and low active ingredient destruction rate, is easy to operate, and only needs to control the operation pressure, the flow speed and the filtrate concentration, and for unstable beta-glucan with relatively large molecular weight, wide distribution range and large solution viscosity, the process is a separation means with great development potential.

Description

technical field [0001] The invention belongs to the field of chemical production, and relates to separation, purification and extraction technology, in particular to a production process for separating and purifying β-glucan in oat bran based on ultrafiltration membrane technology. Background technique [0002] As a food crop, oats are rich in protein, starch, fat, dietary fiber, minerals, etc., and the content of various nutrients is higher than that of other cereal crops, and bran contains a high proportion of protein and dietary fiber. Oat dietary fiber has the function of lowering cholesterol and low-density lipoprotein. Relevant studies have shown that the various functions of oat dietary fiber are mainly realized by β-glucan. [0003] The active structure of β-glucan is a polysaccharide composed of glucose units. Recent studies have found that β-1,3-glucan can control cell division and differentiation, dissolve cholesterol, prevent hyperlipidemia and resist infection ...

Claims

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Application Information

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IPC IPC(8): C08B37/02B01D61/18B01F11/00B01F13/10
CPCC08B37/0003C08B37/0024B01D61/18B01D61/145B01F31/441B01F33/82
Inventor 刘俚扬蔡瑶
Owner 植萃优品(天津)生物科技有限公司
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