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Microbial agent with disease prevention function and preparation method thereof

A microbial agent and functional technology, applied in the field of microbial agent with disease prevention function and its preparation, can solve the problems of reduced yield and quality of flue-cured tobacco, aggravation of soil-borne diseases, etc., achieve quality improvement, root system development, inhibition of pathogenic bacteria and The effect of harmful bacteria

Inactive Publication Date: 2020-04-10
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

By adjusting the composition and structure of microorganisms in the soil, rhizosphere and roots, this product increases the number of beneficial microorganisms, effectively inhibits pathogenic bacteria and harmful bacteria, and solves the problems of aggravation of soil-borne diseases and decline in the yield and quality of flue-cured tobacco caused by repeated cropping of flue-cured tobacco, so as to achieve The purpose of developing tobacco root system, vigorous growth, strong stress resistance and quality improvement

Method used

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  • Microbial agent with disease prevention function and preparation method thereof
  • Microbial agent with disease prevention function and preparation method thereof

Examples

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preparation example Construction

[0028] (1) Preparation of spore liquid, get Bacillus amyloliquefaciens and Bacillus velesii and inoculate them respectively in the liquid fermentation medium under aseptic conditions, cultivate them for 24-36h at 25-37°C, then press 10- 20% volume ratio (bacteria and fermentation broth) was inoculated again in a new liquid fermentation medium, expanded and cultivated at 25-37°C for 24-36h, and then the fermentation broth of functional strains was centrifuged to remove the lower layer of spore liquid for subsequent use;

[0029] (2) Preparation of compound functional bacteria spore liquid, the spore liquid prepared in step (1) has a spore content of more than 95%, effective viable bacteria number ≥ 50 billion cfu / ml, Bacillus amyloliquefaciens and Bacillus Veles After the spore liquid is mixed according to the volume ratio of 1-5:1-5, the compound functional bacteria spore liquid is obtained;

[0030] (3) Material preparation: take protein powder, mannitol, and 20-50 parts, 3-5...

specific Embodiment 1

[0037] A microbial agent with disease prevention function, which comprises the following raw materials in parts by weight: 20 parts of protein powder, 50 parts of calcium magnesium phosphate fertilizer, 50 parts of calcium hydrogen phosphate, 3 parts of mannitol, 3 parts of ferrous sulfate heptahydrate, 1 part of magnesium sulfate water, 700 parts of rapeseed cake organic fertilizer, 5 parts of compound functional bacteria spore liquid.

[0038] Specific embodiment 1

[0039] A microbial agent with disease prevention function, which comprises the following raw materials in parts by weight: 20 parts of protein powder, 20 parts of calcium magnesium phosphate fertilizer, 50 parts of calcium hydrogen phosphate, 3 parts of mannitol, 1 part of ferrous sulfate heptahydrate, 1 part of magnesium sulfate water, 700 parts of rapeseed cake organic fertilizer, 5 parts of compound functional bacteria spore liquid.

specific Embodiment 2

[0041] A microbial agent with disease prevention function, which comprises the following raw materials in parts by weight: 50 parts of protein powder, 50 parts of calcium magnesium phosphate fertilizer, 100 parts of calcium hydrogen phosphate, 5 parts of mannitol, 3 parts of ferrous sulfate heptahydrate, 3 parts of magnesium sulfate water, 800 parts of rapeseed dry cake organic fertilizer, 10 parts of compound functional bacteria spore liquid.

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Abstract

The invention discloses a microbial agent with a disease prevention function. The microbial agent is prepared from the following raw materials in parts by weight: 20 to 50 parts of protein powder, 20to 50 parts of calcium magnesium phosphate fertilizer, 50 to 100 parts of calcium hydrophosphate, 3 to 5 parts of mannitol, 1 to 3 parts of ferrous sulfate heptahydrate, 1 to 3 parts of magnesium sulfate heptahydrate, 700 to 800 parts of rape cake organic fertilizer and 5 to 10 parts of composite functional bacteria spore liquid. According to the principles of soil microecology and plant nutritionphysiology, an efficient microbial strain for preventing and treating tobacco bacterial wilt and black shank is selected, and a microbial agent is refined by adopting a modern microbial engineering technology. By adjusting the composition and structure of soil, rhizosphere and intraradical microorganisms, the number of beneficial microorganisms is increased, pathogenic bacteria and harmful bacteria are effectively inhibited, and the problems that soil-borne diseases are aggravated and the yield and quality of flue-cured tobacco are reduced due to continuous cropping of flue-cured tobacco aresolved, so that the purposes of developed tobacco root systems, vigorous growth, strong stress resistance and quality improvement are achieved.

Description

technical field [0001] The invention relates to the field of microbial bacterial agents, in particular to a microbial bacterial agent with disease prevention function and a preparation method thereof. Background technique [0002] Agricultural microbial agent refers to the live bacterial preparation made of target microorganisms or effective bacteria after industrial expansion, which can be used for seed dressing and root irrigation, and has the functions of improving soil, restoring soil fertility, maintaining the balance of rhizosphere microorganisms and degrading toxic substances And so on, so it is widely used in the cultivation of crops and soil improvement. Agricultural microbial agents are divided into liquid, powder, and granules according to their dosage forms. Liquid and powder have problems in storage and ineffectiveness in the rhizosphere of crops during production and application, which invisibly increases their application costs. Contents of the invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60
CPCC05B3/00C05G3/00
Inventor 王斌朱永立王靓贤尚海丽盘文政莫夏明李绍董华小兵字文霖
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE