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Tin/Protein Nanohybrid Films and Their Applications

A technology of protein and hybrid membranes, applied in the field of multifunctional tin-doped two-dimensional protein nanomembranes, can solve the problems of metal/protein hybrid materials such as difficulty and low efficiency, and achieve good chemical stability and mechanical stability , The preparation method is simple, the effect of good adhesion

Active Publication Date: 2022-05-17
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This limitation often results in the difficulty and inefficiency of designing and synthesizing metal / protein hybrid materials

Method used

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  • Tin/Protein Nanohybrid Films and Their Applications
  • Tin/Protein Nanohybrid Films and Their Applications
  • Tin/Protein Nanohybrid Films and Their Applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] At room temperature, add 100mg of lysozyme to a 10mL Petri dish filled with 10mL of 10mmol / L pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, and then add 0.08g of SnCl 2 , mix evenly, and the pH of the resulting mixed solution is adjusted to be 1.8 with hydrochloric acid, and a layer of tin / lysozyme nano-hybrid film is clearly floating on the gas-liquid interface within 5min (see Figure 1~3 ). The obtained tin / lysozyme nano-hybrid film has a thickness of 50 nm, good flexibility, Young's modulus of elasticity of 0.2 GPa, and hardness of 0.02 GPa.

Embodiment 2

[0027] At room temperature, add 200mg of lysozyme to a 10mL Petri dish filled with 10mL of 10mmol / L pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, and then add 0.08g of SnCl 2 , mixed evenly, the pH of the resulting mixture was adjusted to 1.8 with hydrochloric acid, and a tin / lysozyme nano-hybrid film was clearly formed at the gas-liquid interface within 5 minutes.

Embodiment 3

[0029] At room temperature, add 50mg of lysozyme to a 10mL Petri dish filled with 10mL of 10mmol / L pH 7.4 4-hydroxyethylpiperazineethanesulfonic acid buffer solution, and then add 0.08g of SnCl 2 , mixed evenly, the pH of the resulting mixture was adjusted to 1.8 with hydrochloric acid, and a tin / lysozyme nano-hybrid film was clearly formed at the gas-liquid interface within 5 minutes.

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Abstract

The invention discloses a tin / protein nano-hybrid film and its application. The hybrid film is made by Sn 2+ A tin / protein composite nanofilm formed by reducing protein disulfide bonds to induce protein self-assembly, and the protein is lysozyme, bovine serum albumin, insulin or β-lactoglobulin. The preparation method of the hybrid membrane of the present invention is simple and fast, and it is easy to realize large-area preparation, and it has good biocompatibility, no obvious toxicity to cells, good chemical stability, mechanical stability, and optical transparency. As well as good adhesion and flexibility, it can be adhered to various substrate surfaces. The hybrid membrane of the present invention has a good inhibitory effect on Escherichia coli, Staphylococcus aureus and Candida albicans, and has good catalytic performance at the same time. It can be used as a photocatalytic material to photocatalytically decompose water to produce hydrogen under the irradiation of visible light, and can also be used as an electrocatalyst. Catalytic materials to prepare conductive polymer films.

Description

technical field [0001] The present invention relates to a multifunctional tin-doped two-dimensional protein nanofilm, and multiple uses of the film in micropatterning, antibacterial and photo / electrocatalysis. Background technique [0002] The self-assembly of peptides and proteins to create structurally defined and stable two-dimensional (2D) nanoscale assemblies has important biological and technological implications. Proteins have greater structural and chemical complexity than peptide-based systems, and thus, the assembly of proteins into supramolecular structures is often non-trivial and more challenging than the process for peptides. In particular, its assembly into a macroscopic protein 2D material with a larger scale and uniformity, compared with the general large-scale natural objects, remains a challenge. Furthermore, low assembly efficiency and speed hinder scalable production. [0003] Metal ions play a vital role in more than one-third of natural proteins in n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J5/18C08L89/00C08K3/16G03F7/004C08G73/06A01N59/16A01N37/46A01P1/00A01P3/00B01J31/06C25B1/04C25B1/55C25B11/052C25B11/091C01B3/04
CPCC08J5/18G03F7/004C08G73/0611A01N59/16A01N37/46B01J31/069C25B1/04C01B3/042C08J2389/00C08K3/16C25B1/55C25B11/095B01J35/39B01J35/33Y02E60/36
Inventor 杨鹏巴萨姆·赛义夫
Owner SHAANXI NORMAL UNIV
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