Cottonrose hibiscus endogenous cytokinin extracting agent and using method thereof

A technology of cytokinin and hibiscus, which is applied in the direction of material separation, material analysis, measuring devices, etc., can solve the problems of large amount of organic solvent usage, complicated extraction methods, and low extraction rate, so as to promote water phase distribution and simple technical scheme Feasible and simple extraction method

Pending Publication Date: 2020-04-10
成都市植物园 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when extracting endogenous hormones in the current existing technology, there are generally large amounts of organic solvents used, complex extraction methods, high costs and low extraction rates, and it is difficult to avoid the impact of non-target compounds such as phenols, pigments, proteins, lip

Method used

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  • Cottonrose hibiscus endogenous cytokinin extracting agent and using method thereof
  • Cottonrose hibiscus endogenous cytokinin extracting agent and using method thereof
  • Cottonrose hibiscus endogenous cytokinin extracting agent and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0040] Example 1

[0041] A kind of hibiscus hibiscus endogenous cytokinin extractant, which is prepared by mixing agent A and agent B at a mass ratio of 1.5:1;

[0042] The agent A is uniformly mixed with 0.7505g glycine, 50mL, 0.2mol / L hydrochloric acid and 150mL water, the pH is detected to be 2.0, and it is stored at low temperature;

[0043] The agent B is methanol;

[0044] The method of using Hibiscus mutiflora endogenous cytokinin extractant is as follows:

[0045] a. Preparation of Hibiscus hibiscus endogenous cytokinin extractant: Take the above-mentioned newly configured Hibiscus hibiscus endogenous cytokinin extractant, store it at low temperature, and stand by;

[0046] b. Take 5g of tissue fragments of Hibiscus hibiscus petals, leaves, new roots and 1-year-old branches respectively, add 25mL of Hibiscus hibiscus endogenous cytokinin extractant from step a and mix, and let stand for 4 hours at a temperature lower than 10℃. Filtrate I and filter residue I were obtained by su...

Example Embodiment

[0048] Example 2

[0049] A kind of Hibiscus hirsutum endogenous cytokinin extractant, which is prepared by mixing agent A and agent B in a mass ratio of 1:1;

[0050] The agent A is made up of 0.2054g of phthalic acid, 4.07mL, 0.2mol / L hydrochloric acid and 10mL of double-distilled water evenly mixed, the volume is adjusted to 20mL, the pH is detected to be 2.2, and it is stored at low temperature;

[0051] The agent B is methanol.

[0052] The method of using Hibiscus hibiscus endogenous cytokinin extractant is as follows:

[0053] a. Preparation of Hibiscus hibiscus endogenous cytokinin extractant: Take the above-mentioned newly configured Hibiscus hibiscus endogenous cytokinin extractant, store it at low temperature, and stand by;

[0054] b. Take 5g of tissue fragments of Hibiscus hibiscus petals, leaves, new roots and 1-year-old branches respectively, add 25mL of Hibiscus hibiscus endogenous cytokinin extractant from step a, and mix, and let stand for 8h at a temperature lower than...

Example Embodiment

[0056] Example 3

[0057] A kind of Hibiscus hirsutum endogenous cytokinin extractant, which is prepared by mixing agent A and agent B in a mass ratio of 2:1;

[0058] The agent A is dissolved by 2.1g citric acid, 0.84g sodium hydroxide (97wt%), 1.6g hydrochloric acid (40wt%), 50mL redistilled water, and dilute to 100mL, test pH is 2.2, and store at low temperature;

[0059] The agent B is methanol.

[0060] The method of using Hibiscus hibiscus endogenous cytokinin extractant is as follows:

[0061] a. Preparation of Hibiscus hibiscus endogenous cytokinin extractant: Take the above-mentioned newly configured Hibiscus hibiscus endogenous cytokinin extractant, store it at low temperature, and stand by;

[0062] b. Take 5g of tissue fragments of Hibiscus hibiscus petals, leaves, new roots and 1-year-old branches respectively, add 50mL of Hibiscus hibiscus endogenous cytokinin extractant from step a and mix, and let stand for 8h at a temperature lower than 10℃. Filtrate I and filter residu...

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Abstract

The invention belongs to the technical field of endogenous hormone extraction, and particularly relates to a cottonrose hibiscus endogenous cytokinin extracting agent and a using method thereof. The cottonrose hibiscus endogenous cytokinin extracting agent is prepared by mixing an agent A and an agent B according to a mass ratio of (1-2):1, wherein the agent A is a mixed aqueous solution with thepH value of 2-3 and prepared by selecting at least two of glycine, phthalic acid, disodium hydrogen phosphate, citric acid, sodium citrate, sodium hydroxide, acetic acid, sodium acetate and hydrochloric acid and dissolving the two raw materials with redistilled water; and the agent B is methanol. The cottonrose hibiscus endogenous cytokinin extracting method is simple and low in cost, the extraction rate of the endogenous cytokinin from the hibiscus mutabilis is up to 86-95%, the requirements of detection methods universally accepted in the industry are met, and the technical scheme is simple,feasible and more suitable for popularization.

Description

Technical field [0001] The invention belongs to the technical field of endogenous hormone extraction, and specifically relates to a Hibiscus hibiscus endogenous cytokinin extractant and a use method thereof. Background technique [0002] Phytohormones refer to natural organic compounds produced by metabolism or biosynthesis in plants during normal development or under special environmental conditions. They produce obvious physiological reactions in very small concentrations, which can differentiate, form, grow, and develop in the tissues and organs of plants. Senescence and other physiological processes play an important regulatory role, and also affect important traits such as crop yield, quality and resistance. Currently, the six recognized endogenous plant hormones are auxins, gibberenllins (GA), cytokinins (CK), ethylene, and abscisic acid (ABA). ), Brassinosteroids (Brassinosteroids, BRs). [0003] Hibiscus mutabilis Linn., also known as hibiscus flower, frost-resistant flow...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/14G01N30/72G01N2030/027G01N2030/062Y02A50/30
Inventor 刘晓莉夏中梅王强锋林杨胡凤科徐远超马娇刘兴凤朱章顺侯勇
Owner 成都市植物园
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