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Cellulose degrading bacterium n3 for producing IAA and application thereof

A technology of cellulose-degrading bacteria and bacterial suspensions, applied in the field of agricultural microorganisms, to achieve the effects of accelerating cellulose degradation, promoting the efficiency of straw returning to the field, and increasing crop yields

Active Publication Date: 2020-04-24
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, most scholars at home and abroad only study the cellulose degradation ability or growth-promoting ability of a certain strain, and are committed to studying a certain function of a certain strain, but few strains with multiple functions

Method used

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  • Cellulose degrading bacterium n3 for producing IAA and application thereof
  • Cellulose degrading bacterium n3 for producing IAA and application thereof
  • Cellulose degrading bacterium n3 for producing IAA and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 1. Reagent preparation:

[0055] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, adjust the pH to 7.0-7.2, and sterilize at 121°C for 20 minutes (the solid medium adds 20g agar to this formula).

[0056] Inorganic salt culture medium: ammonium sulfate 2.0g, sodium dihydrogen phosphate 0.5g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate heptahydrate 0.2g, calcium dichloride 0.1g, distilled water 1000mL, adjust pH to 7.0~7.2, and kill at 121℃ Bacteria for 20 minutes.

[0057] Liquid fermentation medium: 6g sodium chloride, 0.1g magnesium sulfate heptahydrate, 0.1g calcium chloride, 0.5g potassium dihydrogen phosphate, 10g yeast extract, 20g straw, 1000mL distilled water, sterilized at 121°C for 20 minutes.

[0058] Enrichment medium: 20g sodium carboxymethylcellulose, 5g microcrystalline cellulose, 5g cellulose powder, 1g dipotassium hydrogen phosphate, 1g nitric acid, 0.2g magnesium sulfate heptahydrate, 0.1g copper chloride dihydr...

Embodiment 2

[0082] The effects of different pH, ventilation, and nitrogen sources on the ability of strains to produce CMC enzymes

[0083] 1. The influence of the initial pH of the medium on the ability of CMC enzyme production

[0084] The strain was inoculated into a liquid fermentation medium with wheat straw powder as the sole carbon source, cultured in a liquid shake flask at 37°C for 60 hours, and its OD was measured with a spectrophotometer 520 value. The initial pH was set to 4, 5, 6, 7, 8, 9, 10, and after 60 hours of cultivation, the content of CMC enzyme produced was measured with a spectrophotometer.

[0085] The result is figure 2 Shown: when the pH value is 5.0, the CMC enzyme activity is the highest, reaching 24.96U·mL -1 , When the pH value is 4.0, the enzyme activity is the second, 24.85U·mL -1 , Indicating that the n3 strain has strong acid tolerance, and the CMC enzyme activity at pH 4.0 and 5.0 is significantly higher than that under other pH conditions.

[0086] 2. The effe...

Embodiment 3

[0093] Test the effects of different pH, ventilation, different time, different carbon sources, and different nitrogen sources on the yield of strain IAA and growth of strain

[0094] 1. The influence of fermentation time on strain IAA yield and strain growth

[0095] Will contain 100mg·L -1 50mL LB liquid medium (IAA detection medium) of L-tryptophan is placed in a 250mL Erlenmeyer flask, and a bacterial suspension with an OD value of about 1 is inoculated according to the inoculum amount of 1% (V / V), 30℃, 180rpm Shaker culture, dynamic sampling at 10, 15, 20, 32, 44, and 56h respectively to determine the growth of strains (OD 600 ) And IAA capacity (OD 530 ), three repetitions for each treatment.

[0096] The result is Figure 5 Shown: OD at 20h 600 After 20h, the growth of the strain appears to decline. The content of IAA produced is basically the same as the growth of the strain. At 10-15h, the content of IAA produced by the strain increases logarithmically, and the IAA content r...

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Abstract

The invention provides a cellulose degrading bacterium n3 for producing IAA and application thereof, and relates to the technical field of agricultural microorganisms. The cellulose degrading bacterium n3 is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 18613. The cellulose degrading bacterium n3 has high CMC enzyme production capacity and can produce IAA, the highest CMC enzyme activity can reach 24.96 U / mL, and the highest secretion amount of IAA can reach 19.07 mg / L. Therefore, the cellulose degrading bacterium n3 can be usedfor preparing a straw decay-promoting microbial inoculum capable of producing IAA and / or CMC enzymes or having a growth-promoting function, so that the cellulose degrading bacterium n3 is applied to straw decay promotion and crop growth promotion, and the efficiency of straw returning to the field and the crop yield are improved.

Description

Technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and specifically relates to an IAA-producing cellulose degrading bacterium n3 and its application. Background technique [0002] Returning straw to the field is a measure to increase the yield of fertile crops that is generally valued in the world today. It eliminates the air pollution caused by the straw burning process and can also improve soil physical properties, increase soil organic matter levels, and increase soil biology. It plays an important role in activity and improving the level of soil nutrient supply. Straws are rich in organic matter and nutrient elements such as nitrogen, phosphorus and potassium, which can be converted into nutrient forms that can be easily absorbed and utilized by crops. Making full use of the positive effects of returning straw to the field can effectively improve resource utilization, increase crop production, and promote nutrient cycling. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/00C12P17/10C05F11/08C12R1/01
CPCC12N9/00C12P17/10C05F11/08C12R2001/01C12N1/205A01N63/20C05F11/00C12P17/12C12P19/02C12P19/14A01P21/00C12N1/20A01N25/04
Inventor 马超吴婧聂彩娥王玉宝吴凉萍张子赟柴如山田达朱林郜红建
Owner ANHUI AGRICULTURAL UNIVERSITY
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