RDA method and kit for rapidly detecting influenza A virus

An influenza A virus and kit technology, applied in the field of molecular biology, can solve the problems of lack of influenza A detection kits, difficulty in obtaining, hindering rapid qualitative detection of influenza, etc., and achieve low detection cost, low mass production cost, The effect of high-precision rapid molecular detection
CN111118219APending Publication Date: 2020-05-08GUANGZHOU PLUSLIFE TECH CO LTD
9 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU PLUSLIFE TECH CO LTD
Publication Date
2020-05-08

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an RDA method and a kit for rapidly detecting influenza A virus. The kit comprises a specific primer group and an RDA fluorescence labeling probe so as to realize safe, specific, sensitive, simple and convenient detection of the influenza A virus (FluA), and to make up for the defects of existing traditional detection technologies. According to the kit provided by the invention, a nucleic acid extraction step can be omitted, the influenza A virus can be detected within 20 minutes at a constant temperature of 37-42 DEG C, the specificity is 100%, and the kit is very suitable for on-site rapid detection. Compared with common PCR methods, the RDA fluorescence method has the advantages that: the reaction is carried out at constant temperature, the temperature change isnot needed, complex instruments are not needed, and the reaction time is short. Therefore, the method and the kit provided by the invention have the characteristics of simple and rapid operation, goodspecificity, high sensitivity, low cost and the like, provide an effective a technical means for on-site rapid detection and screening of the influenza A virus, and have a wide application prospect.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of molecular biology. More specifically, it relates to a primer, a probe and a related kit for detecting influenza A virus nucleic acid based on RDA fluorescence detection technology. Background technique

[0002] Influenza virus belongs to the Orthomyxoviridae family and is a single-stranded RNA membrane virus. It is the most common respiratory virus and is divided into three types: A (A), B (B) and C (C). Influenza A and B are the most common types of influenza virus, and they occur almost once a year in China. Among them, influenza A virus is the most likely to mutate and is highly pathogenic to humans. It has caused worldwide pandemics many times. The structure of influenza A virus can be divided into three parts: envelope, matrix protein and core from the outside to the inside. There are two types of surface glycoproteins on the outer granule membrane. The first type is hemagglutinin (H), which is divi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More