A high-efficiency extraction method for total protein of macadamia nut leaves

An extraction method and protein technology, applied in the field of protein extraction, can solve the problems of low protein purity, easy oxidative browning, etc., achieve the effect of wide distribution of protein size, avoid protein degradation, and clean background
CN111171108BActive Publication Date: 2021-12-10SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
Publication Date
2021-12-10

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Abstract

The invention discloses a high-efficiency extraction method for the total protein of macadamia nut leaves. The method includes the following steps: after crushing the fresh leaves of macadamia nuts with liquid nitrogen, adding pre-cooled lysate PW1 for primary extraction, obtaining the first precipitate after solid-liquid separation, cleaning and drying the first precipitate; adding extraction solution PW2 for secondary extraction Extraction, after solid-liquid separation to obtain the second supernatant, add pre-cooled methanol solution, centrifuge to obtain the second precipitate, wash and dry the second precipitate; add the dissolving solution PW3 for three extractions, and obtain the second precipitate after solid-liquid separation Three supernatants, that is. The present invention uses fresh macadamia nut leaves as materials, and adding three kinds of extracts of the present invention to the macadamia nut leaf powder can effectively avoid raw material oxidation and protein degradation, the total protein yield is high, and the extracted protein has high purity, and SDS-PAGE electrophoresis strips The bands are neat and clear, and suitable for Western Blot analysis.
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Description

technical field

[0001] The invention belongs to the technical field of protein extraction. More specifically, it relates to a high-efficiency extraction method for total protein in leaves of macadamia nuts. Background technique

[0002] Macadamia ternifolia F.Muell., also known as Queensland chestnut, macadamia nut, and macadamia walnut, belongs to the genus Macadamia of the family Longanaceae. It is an evergreen tree native to southeastern Queensland and northern New South Wales, Australia. Coastal subtropical rainforest at 25Β°-31Β° south latitude. Macadamia nuts are rich in nutrients, with an oil content of 70% to 79%, especially rich in unsaturated fatty acids, rich in protein, and rich in phosphorus, calcium, iron, vitamins, amino acids, etc., known as the "Queen of Dried Fruits" and "No. King". At present, research on macadamia at home and abroad is mainly focused on the promotion of cultivation techniques, germplasm identification, resource utilization, etc. There ar...

Claims

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