A functional acellular matrix biomaterial and its preparation method and application
A technology of acellular matrix and biomaterials, applied in the field of functionalized acellular matrix biomaterials and its preparation, can solve the problems that genipin has not been widely used, and improve anticoagulant performance, reduce calcification reaction, and improve The effect of crosslink density
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Embodiment 1
[0037] A method for preparing a functionalized acellular matrix biomaterial provided by a preferred embodiment of the present invention, the specific steps are as follows:
[0038] 1. Obtain fresh porcine pericardium and store it in a humid state at 4°C;
[0039] 2. Soak the animal tissue material in 0.1% EDTA solution for 1 hour, then immerse in 0.1% SDS solution for 24 hours for decellularization to make acellular matrix;
[0040] 3. Use a cleaning solution prepared with a volume ratio of distilled water and alcohol of 1:1, and ultrasonically clean at room temperature to clean the acellular matrix;
[0041] 4. Soak the cleaned acellular matrix in an aqueous solution of 1 wt% methacrylic anhydride and react at 25°C for 24 hours;
[0042] 5. Use the above-mentioned cleaning solution to clean the above-mentioned materials by ultrasonic cleaning at room temperature, and then soak them in 10 mmol / L 3-propyl methacrylate aqueous solution for 24 hours at 25°C;
[0043] 6. Add 5 m...
Embodiment 2
[0045] A method for preparing a functionalized acellular matrix biomaterial provided by a preferred embodiment of the present invention, the specific steps are as follows:
[0046] 1. Obtain fresh porcine pericardium and store it in a humid state at 4°C;
[0047] 2. Soak the animal tissue material in 0.1% EDTA solution for 1 hour, then immerse in 0.1% SDS solution for 24 hours for decellularization to make acellular matrix;
[0048] 3. Use a cleaning solution prepared with a volume ratio of distilled water and alcohol of 1:1, and ultrasonically clean at room temperature to clean the acellular matrix;
[0049] 4. Soak the washed acellular matrix in an aqueous solution of 5 wt% methacrylic anhydride and react at 20°C for 24 hours;
[0050] 5. Use the above-mentioned cleaning solution to clean the above-mentioned materials by ultrasonic cleaning at room temperature, and then soak them in 50mmol / L 3-sulfopropyl methacrylate aqueous solution at 30°C for 24h;
[0051] 6. Add 20 mM...
Embodiment 3
[0053] A method for preparing a functionalized acellular matrix biomaterial provided by a preferred embodiment of the present invention, the specific steps are as follows:
[0054] 1. Obtain fresh porcine pericardium and store it in a humid state at 4°C;
[0055] 2. Soak the animal tissue material in 0.1% EDTA solution for 1 hour, then immerse in 0.1% SDS solution for 24 hours for decellularization to make acellular matrix;
[0056] 3. Use a cleaning solution prepared with a volume ratio of distilled water and alcohol of 1:1, and ultrasonically clean at room temperature to clean the acellular matrix;
[0057] 4. Soak the washed acellular matrix in a 3 wt% aqueous solution of methacrylic anhydride and react at 30°C for 24 hours;
[0058] 5. Use the above-mentioned cleaning solution to clean the above-mentioned materials by ultrasonic cleaning at room temperature, and then soak them in 50mmol / L 3-propyl methacrylate aqueous solution at 37°C for 24h;
[0059] 6. Add 20 mM potas...
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