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Continuous culture method for Dunaliella salina

A cultivation method, the technology of Dunaliella salina, which is applied in the field of continuous cultivation of microalgae, can solve the problems of decreased cultivation efficiency of Dunaliella salina, low survival rate of Dunaliella salina, and unreasonable setting of fermentation time. And the design of the fermentation environment is reasonable, the overall cultivation efficiency is improved, and the effect of improving the quality of the living environment

Inactive Publication Date: 2020-05-29
ZHANJIANG GUOLIAN AQUATIC PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a continuous cultivation method of Dunaliella salina, to solve the unreasonable setting of the fermentation time proposed in the above background technology, which leads to too long cultivation time of early seeds and unreasonable disinfection method, which leads to the survival of Dunaliella salina At the same time, it is impossible to add fresh sterile medium during the cultivation process through continuous cultivation, which leads to the problem that the cultivation efficiency of Dunaliella salina is greatly reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A continuous cultivation method for Dunaliella salina, the cultivation method comprising the following steps:

[0024] A: Prepare medium in a closed photobioreactor, each 1L of medium contains: sodium nitrate, dipotassium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous sulfate and Na-EDTA, 1ml of trace element mother solution and 1ml of Vitamin mother liquor, the pH in the culture medium in step A is between 6.5-8, and the content of sodium nitrate, dipotassium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous sulfate and Na-EDTA is respectively 0.3g, 0.02g . L, microorganism 0.1-2.0mg / L;

[0025] B: Submerge the ultraviolet disinfection lamp tube into the culture medium, and carry out ultraviolet irradiation disinfection. The disinfection time is 10 minutes. The ultraviolet disinfection method in step B is: the ultraviolet wavelength emitted by the ultraviolet disinfection lamp tube is 180-254nm, and the disinfection time of the cultu...

Embodiment 2

[0029] A continuous cultivation method for Dunaliella salina, the cultivation method comprising the following steps:

[0030] A: Prepare medium in a closed photobioreactor, each 1L of medium contains: sodium nitrate, dipotassium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous sulfate and Na-EDTA, 1ml of trace element mother solution and 1ml of Vitamin mother liquor, the pH in the culture medium in step A is between 6.5-8, and the content of sodium nitrate, dipotassium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous sulfate and Na-EDTA is respectively 0.9g, 0.019g . L, microorganism 0.1-2.0mg / L;

[0031] B: Submerge the ultraviolet disinfection lamp tube into the culture medium, and carry out ultraviolet irradiation disinfection. The disinfection time is 10 minutes. The ultraviolet disinfection method in step B is: the ultraviolet wavelength emitted by the ultraviolet disinfection lamp tube is 180-254nm, and the disinfection time of the cult...

Embodiment 3

[0035] A continuous cultivation method for Dunaliella salina, the cultivation method comprising the following steps:

[0036] A: Prepare medium in a closed photobioreactor, each 1L of medium contains: sodium nitrate, dipotassium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous sulfate and Na-EDTA, 1ml of trace element mother solution and 1ml of Vitamin mother liquor, the pH in the culture medium in step A is between 6.5-8, and the content of sodium nitrate, dipotassium hydrogen phosphate, magnesium chloride, calcium chloride, ferrous sulfate and Na-EDTA is respectively in 0.8g, 0.025g . L, microorganism 0.1-2.0mg / L;

[0037] B: Submerge the ultraviolet disinfection lamp tube into the culture medium, and carry out ultraviolet irradiation disinfection. The disinfection time is 10 minutes. The ultraviolet disinfection method in step B is: the ultraviolet wavelength emitted by the ultraviolet disinfection lamp tube is 180-254nm, and the disinfection time of the c...

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Abstract

The invention discloses a continuous culture method for Dunaliella salina. The culture method comprises the following steps: A, preparing a culture medium in a closed photobioreactor; B, immersing anultraviolet disinfection lamp tube into a culture solution, and carrying out ultraviolet irradiation disinfection for 10 minutes; C, selecting a batch of Dunaliella salina cultured to an exponential growth phase, inoculating the Dunaliella salina into the culture medium, inoculating at the temperature that is controlled to be 23-28 DEG C, and alternately culturing for appointed days according to certain illumination time, and D, releasing a certain volume of Dunaliella salina culture solution after the step C, and inoculating a fresh culture solution subjected to ultraviolet disinfection intothe closed reactor. According to the continuous culture method for the Dunaliella salina, the culture efficiency of the Dunaliella salina is improved, the early-stage seed culture time is greatly shortened through continuous fermentation, and meanwhile, an ultraviolet disinfection system is used, which can guarantee that the fresh sterile culture medium is added during the culture process, and guarantee normal proceeding of the continuous culture process.

Description

technical field [0001] The invention relates to the technical field of continuous cultivation of microalgae, in particular to a method for continuous cultivation of Dunaliella salina. Background technique [0002] Dunaliella salina is a kind of microalgae. Salina salina has a good effect on stabilizing blood sugar and blood pressure and improving sleep quality. Dunaliella salina is a kind of halophilic green microalgae, which is often found in sea salt fields. Because of its high food value, so people will increase its output by means of artificial cultivation, so as to meet a large number of market demands, but the existing cultivation methods have the following problems: [0003] The setting of fermentation time is unreasonable, which leads to too long seed cultivation time and unreasonable disinfection method, which leads to low survival rate of Dunaliella salina. At the same time, it is impossible to add fresh sterile medium during the cultivation process through continu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 郭立赵阳娟何加发梁薏琳邵华
Owner ZHANJIANG GUOLIAN AQUATIC PROD CO LTD
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