Application of amomum maximum roxb extract in preparation of alpha-glucosidase inhibitor drugs
A technology of glucosidase and cardamom, applied in the field of medicinal chemistry, to achieve the effects of reducing production and absorption, protecting pancreatic function, and slowing blood sugar rise
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[0032] Example 1 Preparation of compound of formula I
[0033] Take 2 kilograms of dried Nine-winged cardamom rhizome and crush it with 5L 95% ethanol for heating and refluxing for three times. Combine the three extracts and concentrate under reduced pressure until there is no alcohol. Add pure water to a total volume of 1L. Use an equal volume of ethyl acetate. The ester was extracted three times, the ethyl acetate extracts were combined, and concentrated to dryness under reduced pressure.
[0034] The ethyl acetate extract was subjected to silica gel column chromatography, eluting with a petroleum ether-acetone gradient. Collect the petroleum ether-acetone 9:1 elution fraction and recover it to dryness under reduced pressure. After silica gel column chromatography, dichloromethane-methanol gradient elution, the 98:2 elution fraction was collected, and the fraction was separated by preparative liquid chromatography, such as figure 1 Shown is the liquid chromatogram of this fract...
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[0035] Example 2 Preparation of compound of formula I
[0036] Take 2 kilograms of dried Nine-winged cardamom rhizome and pulverize, extract with 20L 95% ethanol, combine the alcohol extracts, concentrate to about 2L under reduced pressure, add 500 grams of pretreated macroporous resin (resin model can be D101, AB -8, HPD-100 and HP20, etc.). After fully volatilizing and removing the ethanol, install the column, ethanol-water gradient elution (0, 30%, 60%, 80% and 95% ethanol), the 80% ethanol eluted fraction is divided into the target fraction, and concentrated to dryness under reduced pressure; pass through silica gel Column chromatography, dichloromethane-methanol gradient elution, collect 98:2 elution fraction, the fraction is separated by preparative liquid chromatography, the mobile phase is methanol-water 85:15, the flow rate is 6ml / min, the column is RP-C18 chromatographic column (10*250mm), the detection wavelength is set to 280nm, and the chromatographic peaks with ret...
Example Embodiment
[0037] Example 3 Structural identification of the compound of formula I
[0038] The structure of the compound of formula I was determined by nuclear magnetic resonance spectrum and mass spectrometry analysis.
[0039] The carbon spectrum and hydrogen spectrum data are as follows:
[0040] 1 H-NMR(600MHz,CDCl3)δ7.67(d,J=6.0Hz,1H), 7.56(s,1H), 6.33(d,J=6.0Hz,1H), 4.82(s,1H), 4.64( s, 1H), 2.69 (d, J = 16.2 Hz, 1H), 2.45 (dd, J = 11.4, 16.2 Hz, 1H), 2.40-2.36 (m, 1H), 2.04-1.95 (m, 2H), 1.89 -1.85(m,1H),1.78-1.73(m,1H),1.64-1.56(tq,J=3.0,13.8Hz,1H),1.55-1.48(m,1H),1.43-1.38(m,1H) ,1.37-1.32(dq,J=4.2,12.6Hz,1H),1.24-1.15(m,3H),0.88(s,3H),0.82(s,3H),0.78(s,3H).
[0041] 13 C-NMR(150MHz,CDCl3)δ178.70(-CO-,C-13), 154.61(=CH-,C-15),152.99(=CH-,C-16),147.70(C,C-8 ),129.85(C,C-12),116.36(=CH-,C-14),107.77(=CH-,C-17),55.54(CH,C-5),53.96(CH,C-9) ,42.06(CH2,C-3),39.98(C,C-10),39.01(CH2,C-1),38.07(CH2,C-7),33.63(CH3,C-18),33.63(C, C-4), 24.37 (CH2, C-6), 21.72 (CH3, C-19), 19.54 (CH2, C-11), ...
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