DNA bar code used for screening high-quality Tibet brown mushrooms, primer and application of DNA bar code used for screening high-quality Tibet brown mushrooms and primer

Abstract

The invention discloses a DNA bar code used for screening high-quality Tibet brown mushrooms, a primer and application of the DNA bar code used for screening the high-quality Tibet brown mushrooms andthe primer. The DNA bar code used for screening the high-quality Tibet brown mushrooms contains one or more of 17 DNA fragments with nucleotide sequences shown in SEQ ID NO: 1-17 as shown in the description. The amplification primer of the DNA bar code used for screening the high-quality Tibet brown mushrooms contains one or more of 17 pairs of primers with upstream and downstream nucleotide sequences respectively shown in SEQ ID NO: 18-51 as shown in the description. Compared with a traditional breeding method and other existing DNA bar code technologies, the DNA bar code used for screeningthe high-quality Tibet brown mushrooms, the primer and application of the DNA bar code used for screening the high-quality Tibet brown mushrooms and the primer have the advantages of being time-saving, energy-saving, money-saving, accurate and efficient, and play a positive role in genetic breeding of the high-quality Tibet brown mushrooms, and an effective method is provided for identification and protection of germplasm resources at the same time.

Description

technical field [0001] The invention relates to the technical field of edible fungus germplasm resources screening, more specifically, to a DNA barcode, primers and applications for screening high-quality Tibetan brown mushrooms. Background technique [0002] Tibetan brown mushroom, belonging to a type of Agaricus bisporus, is light brown in color between brown Agaricus bisporus (brown mushroom) and white Agaricus bisporus. White Agaricus bisporus is easy to brown and affect the quality, while brown Agaricus bisporus is not easy to brown and has high protein content. The main factor causing browning is the activity of polyphenol oxidase in Agaricus bisporus. The higher the activity, the easier browning. Tibetan brown mushroom is not easy to brown and has good quality of both white and brown Agaricus bisporus. The main indicators for evaluating high-quality varieties of edible fungi such as Agaricus bisporus include: high content of total protein, total soluble protein, tota...

AI Technical Summary

Problems solved by technology

It is mainly for the identification of a small number of genes or fragments, resulting in a small amount of information and poor identification effect, and it cannot be used for identification and screening of good quality features

In the existing DNA barcoding technology, the restriction fragment length polymorphism (RFLP) operation steps are cumbersome and complicated, and radioactive isotope labels or carcinogenic imaging markers are used at the same time, the amount of genome templates is large, and there is little band type information The amount is small, and at the same time, it is easily affected by gene mutations, resulting in unstable results

Randomly amplified polymorphic DNA (RAPD) in the existing DNA barcoding technology is susceptible to interference, and requires high technical level and stability repeatability of operators. In addition, the quality and concentration of the sample genome template, The length and sequence of primers, the number of PCR cycles, the complexity of genomic DNA, and technical equipment may all lead to poor repeatability of RAPD technology.

The single nucleotide polymorphism (SNP) technology in the existing DNA barcoding technology relies on SNP chip or mass spectrometry and sequencing technology, which requires high equipment, high price and high cost

Therefore, in view of the shortcomings of traditional breeding methods that are not accurate enough to be time-consuming and labor-intensive in breeding Tibetan brown mushroom varieties, it is necessary to provide a DNA barcoding technology that can accurately and quickly identify the species of Tibetan brown mushrooms, and at the same time realize high-quality breeding. The defect of DNA barcode technology provides a detection method with low cost, high efficiency, easy operation, stable and reliable results and good repeatability

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