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Composite-aperture electrostatic spinning bracket modified with slow-release desferrioxamine microbubbles and preparation method of composite-aperture electrostatic spinning bracket

An electrospinning and deferoxamine technology, which is applied in the field of composite pore electrospinning scaffold modified by deferoxamine slow-release microbubbles and its preparation, can solve the problems of few relevant research reports on the release of activating factors, and achieve improved release The effect of the characteristic

Active Publication Date: 2020-06-09
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pore expansion method usually only achieves the purpose of pore expansion, and there are few relevant research reports on the release of activating factors.

Method used

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  • Composite-aperture electrostatic spinning bracket modified with slow-release desferrioxamine microbubbles and preparation method of composite-aperture electrostatic spinning bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Preparation of deferrioxamine sustained-release microbubbles

[0029] Add 8g lactide, 2g glycolide, 1g polyethylene glycol 2000 to the polymerization tube, then add 0.5g of 0.3% stannous octoate in dichloromethane as a catalyst, and heat the polymerization tube to 150°C under vacuum to mix The material is melted and mixed uniformly, and kept for 8 hours. After the temperature is naturally cooled, it is dissolved in acetone, precipitated with water, and washed to obtain the polymer as the shell material. The hydrophilicity test of the polymer prepared above has a contact angle of 30°, a viscosity of 1.2, and a breaking elongation of 4.5%.

[0030] Dissolve 0.5 g of deferoxamine in 10 mL of water as the internal water phase, and then dissolve 5 g of polymer in 100 mL of ethyl acetate as the oil phase, and disperse the internal water phase in the oil phase with sufficient stirring to obtain a W / O emulsion; 10gPVA and 25gNaCl are dissolved in 500mL water as the external wat...

Embodiment 2

[0036] 1) Preparation of desferrioxamine sustained-release microbubbles: This step is the same as in Example 1.

[0037] 2) Preparation of the electrospinning scaffold modified by deferrisensitized slow-release microbubbles

[0038] The core liquid of electrospinning is composed of deferrioceptor sustained-release microbubbles, type I collagen, and hexafluoroisopropanol. The mass content of deferrioceptor sustained-release microbubbles is 10%, and the mass content of type I collagen is 5%; electrospinning The shell liquid of the silk is composed of hydroxyapatite, type I collagen, and hexafluoroisopropanol. The mass content of hydroxyapatite is 15%, and the mass content of type I collagen is 15%. It is prepared by coaxial electrospinning method. , The electrostatic high voltage is 50 kV, the receiving distance is 200mm, the electrospinning rate is 0.05ml / min, and the desferrioceptor static slow-release microbubble modified electrospinning support is prepared.

[0039] 3) Laser reami...

Embodiment 3

[0042] 1) Preparation of desferrioxamine sustained-release microbubbles: This step is the same as in Example 1.

[0043] 2) Preparation of the electrospinning scaffold modified by deferrisensitized slow-release microbubbles

[0044] The core liquid of electrospinning is composed of deferrisensitized sustained-release microbubbles, type I collagen, and hexafluoroisopropanol. The mass content of deferrisensitized sustained-release microbubbles is 1%, and the mass content of type I collagen is 15%; The shell liquid of the silk is composed of hydroxyapatite, type I collagen, and hexafluoroisopropanol. The mass content of hydroxyapatite is 5%, and the mass content of type I collagen is 5%; it is prepared by coaxial electrospinning method. , The electrostatic high voltage is 1 kV, the receiving distance is 50mm, the electrospinning rate is 0.2ml / min, and the desferrioceptor static slow-release microbubble modified electrospinning support is prepared.

[0045] 3) Laser reaming to prepare c...

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Abstract

The invention relates to a composite-aperture electrostatic spinning bracket modified with slow-release desferrioxamine microbubbles and a preparation method of the composite-aperture electrostatic spinning bracket. Glycolide and / or lactide are / is adopted as monomers for a polymerization reaction to prepare a polymer, the polymer is taken as a shell material, desferrioxamine is used as a core material, and the slow-release desferrioxamine microbubbles with the core-shell structure are prepared with a rapid emulsification method; a core liquid containing the slow-release desferrioxamine microbubbles and a shell liquid are subjected to coaxial electrostatic spinning, and an electrostatic spinning bracket modified with the slow-release desferrioxamine microbubbles is obtained; the electrostatic spinning bracket is irradiated by laser for reaming, and the composite-aperture electrostatic spinning bracket modified with the slow-release desferrioxamine microbubbles is obtained. The bionic bracket with 10-500 micrometer composite aperture is obtained, good conditions are provided for growth of new bone and blood vessels, furthermore, release of the desferrioxamine is realized, sufficientconcentration can be obtained at an early stage, and a long-term slow-release effect can be kept.

Description

Technical field [0001] The present application relates to a tissue scaffold for bone repair and a preparation method thereof, and in particular to a composite aperture electrospinning scaffold modified with deferrioceptive slow-release microbubbles and a preparation method thereof. Background technique [0002] Critical bone defect repair is a thorny clinical problem. At present, the gold standard for critical size bone defect treatment is autologous bone grafting. However, the amount of bone tissue in the donor site is limited, and various complications such as postoperative bone defect, pain, and infection in the donor site severely limit the popularization and application of autologous bone transplantation. In the repair of critical-size bone defects, insufficient graft vascularization can easily lead to the formation of dead bone and difficult recovery of limb function. Therefore, the development of grafts with good vascularization and functionalization has become a hot resea...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/18A61L27/50D01D5/00D01D5/34
CPCA61L27/18A61L27/50A61L27/54A61L2300/204A61L2300/412A61L2300/602A61L2300/622A61L2430/02D01D5/0015D01D5/34C08L67/04
Inventor 崔翔刘建恒李明刘鐘阳孙国飞张里程唐佩福
Owner GENERAL HOSPITAL OF PLA
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