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Application of annona squamosa seed total lactones to preparation of medicines for controlling diabetes

A technology of custard lactone and total lactone, applied in the application field of medicine, can solve problems such as adverse reactions, rebound, and pollute the environment, and achieve the effect of broadening clinical activity

Inactive Publication Date: 2020-06-23
JIANGSU HEALTH VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many drugs for the treatment of diabetes and its complications. Although the western medicines used clinically have certain curative effects, most of them have relatively large adverse reactions, such as rebound after drug withdrawal, liver and kidney damage, rhabdomyolysis and other side effects. , not the root cause
[0003] Annona squamosa (Annona squamosa) is a plant of the genus Annonaceae (Annonaceae). It is widely planted in Guangdong, Guangxi, Yunnan and Hainan in southern China. The seeds of Annona squamosa are discarded after people eat the fruit of Annona squamosa , not used in further processing, and pollute the environment
Pharmacological experiments of annonaceae lactones have shown that they have strong anticancer, insecticidal, parasitic, and antibacterial pharmacological activities. In the past 30 years, scholars at home and abroad have isolated nearly 500 annonaceae from plants of the family Annonaceae. Ester compounds, the current research is mainly focused on the anti-tumor activity of anemone lactones, and there is no report on the application of total lactones of anemone apple seeds in the treatment of diabetes and diabetic nephropathy

Method used

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  • Application of annona squamosa seed total lactones to preparation of medicines for controlling diabetes
  • Application of annona squamosa seed total lactones to preparation of medicines for controlling diabetes
  • Application of annona squamosa seed total lactones to preparation of medicines for controlling diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A kind of preparation method of anemone lactone for treating diabetic nephropathy, it comprises the following steps:

[0045] Take the custard apple seeds, crush them through a 80-mesh sieve, add ethanol in an amount 10 times the weight of the custard apple seeds, extract twice by reflux, each time for 2 hours, filter, combine the filtrates, concentrate to obtain a concentrate, and put it on the large hole D101 Hole resin column, first eluted with 10% ethanol for 6 times column volume, then 50% ethanol for 6 times column volume, and then chloroform for 10 times column volume, collected chloroform eluted parts, concentrated to obtain custard apple seeds Total lactones of custard apple.

Embodiment 2

[0047] A kind of preparation method of anemone lactone for treating diabetic nephropathy, it comprises the following steps:

[0048] Take the custard apple seeds, crush them through an 80-mesh sieve, and use supercritical CO 2 Extraction of anemone lactones from custard apple seeds; supercritical CO 2 The extraction process is a pressure of 25Mpa, an extraction temperature of 35°C, and an extraction time of 3 hours. The entraining agent is ethanol, and the amount of ethanol is 20% of the amount of medicinal materials.

Embodiment 3

[0049] Example 3 Lowering Blood Sugar, Lowering Blood Fat and Protecting Kidney Damage Experiments

[0050] 1. Test drug:

[0051] The total annona lactones prepared in Example 1, the compounds bullatacin, squamostatin and amurisolin;

[0052] 2. Experimental method:

[0053] (1), get SD rats, body weight (220 ± 20) g, male barrier environment rats (SPF grade), adaptive feeding 5 days, during the experiment, 10 rats in the blank group were fed with conventional feeds, and the rest of the rats were fed with Feed high-sugar, high-fat and 0.5% glucose water. After 3 weeks, inject STZ intraperitoneally at 30 mg / kg for 3 consecutive days. After feeding with high-sugar, high-fat and glucose water for 4 days, the rats were fasted and could not hold water. Blood was taken to measure fasting blood glucose, and the modeling was successful when the blood glucose value was higher than 13mmol / L. The diabetic rats successfully modeled were randomly divided into a normal group, a model gr...

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PUM

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Abstract

The invention discloses an application of annona squamosa seed total lactones and annona squamosa pivalolactone monomeric compounds thereof to preparation of medicines for controlling diabetes and diabetic nephropathy thereof. Through massive experimental selection, results show that the annona squamosa seed total lactones and the annona squamosa pivalolactone monomeric compounds thereof have theefficacy of reducing fasting blood sugar of rats suffering from diabetic nephropathy, the content of serum creatinine, the content of urea nitrogen and the content of urine protein of the rats suffering from the diabetic nephropathy can be notably reduced, and the annona squamosa seed total lactones and the annona squamosa pivalolactone monomeric compounds thereof have good effects of reducing blood sugar and protecting the kidney.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to the application of total lactones of custard apple seeds in the preparation of medicines for preventing and treating diabetes. Background technique [0002] Diabetes is a common and frequently-occurring disease worldwide, and has become the third largest disease after tumors and cardiovascular diseases. Clinically common diabetic heart disease and diabetic nephropathy. At present, there are many drugs for the treatment of diabetes and its complications. Although the western medicines used clinically have certain curative effects, most of them have relatively large adverse reactions, such as rebound after drug withdrawal, liver and kidney damage, rhabdomyolysis and other side effects. , Treat the symptoms but not the root cause. [0003] Annona squamosa (Annona squamosa) is a plant of the genus Annonaceae (Annonaceae). It is widely planted in Guan...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K31/365A61P3/10A61P13/12A61K131/00
CPCA61K31/365A61K36/185A61K2236/33A61K2236/37A61K2236/55A61P3/10A61P13/12
Inventor 杨海军张宁
Owner JIANGSU HEALTH VOCATIONAL COLLEGE
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