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Kit for detecting inactivated viruses and detection method

A virus detection and kit technology, applied in the biological field, can solve the problems of high infection risk, inability to detect, and easy to destroy for medical staff and testing personnel, and achieve the effects of high sensitivity and accuracy, improved stability, and accurate test results.

Active Publication Date: 2020-06-23
浙江大学医学院附属第四医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

They all need to collect fresh samples (pharyngeal swabs, sputum, lung perfusate or blood) from patients for testing, but these samples can still pose a great risk of infection to medical staff and testing personnel
Immediately inactivating the collected samples can effectively reduce the risk of infection for medical staff and testing personnel. However, after the samples are inactivated (usually using an autoclave), although the virus loses its original pathogenicity, its Detection markers (DNA, RNA, and proteins with spatial structure) are also easily damaged and cannot be detected

Method used

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  • Kit for detecting inactivated viruses and detection method
  • Kit for detecting inactivated viruses and detection method

Examples

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Embodiment 1

[0041] A test kit for inactivated virus detection, comprising the following components: (1) buffer: ammonium bicarbonate solution with a concentration of 50mmol / L, (2) reducing agent: dithiothreitol solution with a concentration of 20mmol / L , (3) alkylating reagent: iodoacetamide solution of concentration 60mmol / L, (4) trypsin: alkaline trypsin, its concentration is 100mg / L, (5) stop solution: the formic acid of mass concentration 60% , (6) Thermostable standard polypeptide; the amino acid sequence of said thermostable standard polypeptide is: LPLGINITNFR (SEQ ID No.1), and the concentration is 20ng / ml.

Embodiment 2

[0043] A test kit for inactivated virus detection, comprising the following components: (1) buffer: ammonium bicarbonate solution with a concentration of 1000mmol / L, (2) reducing agent: dithiothreitol solution with a concentration of 200mmol / L , (3) alkylating reagent: iodoacetamide solution of concentration 600mmol / L, (4) trypsin: alkaline trypsin, its concentration is 1000mg / L, (5) stop solution: the formic acid of mass concentration 98% , (6) Thermostable standard polypeptide; the amino acid sequence of said thermostable standard polypeptide is: LPLGINITNFR (SEQID No.1), and the concentration is 1000ng / ml.

Embodiment 3

[0045] A test kit for inactivated virus detection, comprising the following components: (1) buffer: ammonium bicarbonate solution with a concentration of 500mmol / L, (2) reducing agent: dithiothreitol solution with a concentration of 100mmol / L , (3) alkylating reagent: the iodoacetamide solution of concentration 300mmol / L, (4) trypsin: alkaline trypsin, its concentration is 500mg / L, (5) stop solution: the formic acid of mass concentration 98% , (6) Thermostable standard polypeptide; the amino acid sequence of said thermostable standard polypeptide is: LPLGINITNFR (SEQID No.1), and the concentration is 300ng / ml.

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Abstract

The invention discloses a kit for detecting inactivated viruses. The kit comprises the following components (1) a buffer solution, (2) a reducing agent, (3) an alkylation reagent, (4) trypsin, (5) a stop solution, and (6) a thermally stable standard polypeptide. An amino acid sequence of the thermally stable standard polypeptide is as follows: LPLGINITNFR. Under the condition of ensuring detectionsensitivity and specificity, the inactivated viruses can be detected, and an accurate detection result is obtained.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a detection method for detecting inactivated viruses. Background technique [0002] Viruses are a class of non-cellular microorganisms. The mature and complete virus particle becomes the virion, which is composed of nucleic acid and protein. Viruses are characterized by their small size, which can pass through a sterile filter; simple structure, containing only one type of nucleic acid (DNA or RNA); lack of genes encoding mitochondria and ribosomes, and must be parasitic in living cells and replicated Breed offspring. [0003] Since the first discovery of tobacco mosaic virus in 1892, more than 4,000 species of animal and plant viruses have been discovered, of which more than 500 species are pathogenic to humans. In clinical microbial infections, those caused by viruses account for about 75%. Some infectious guns spread rapidly and widely, with high mortality and serious...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86G01N33/68
CPCG01N30/02G01N30/8675G01N33/6848Y02A50/30
Inventor 吴建国朱维罡吴伟根吴英萍李游江
Owner 浙江大学医学院附属第四医院