Microbial agent for repairing cadmium-contaminated soil and preparation method and application thereof

A microbial agent, cadmium-contaminated soil technology, applied in the field of soil remediation, can solve the problems of lack of nutrients, increase the complexity of operation and cost, etc., achieve the effect of low price, convenient large-scale production, and increase the load point

Pending Publication Date: 2020-07-03
YUNNAN SHENGQING ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common inorganic fillers include Pall rings, ceramsite, etc. Although they have good mechanical properties, they do not have any nutrients themselves. During the process, nutrients and pH regulators are often added regularly, which increases the complexity and cost of the operation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0029] A method for preparing a microbial agent for remediating cadmium-contaminated soil according to a first aspect embodiment of the present invention comprises the following steps:

[0030] 1. Preparation of cellulose-based carrier

[0031] Ⅰ. Make a mixed solution of NaOH, urea, and water with a mass ratio of 3-7:3-5:80-100, add 10%-20% cellulose by mass, and store at 0°C-5°C for 6-12 Hour, obtain cellulose solution;

[0032] Ⅱ. Add 5%-10% CaCO to the cellulose solution 3 Ultrasonic treatment to obtain a mixed solution of porous cellulose pellets;

[0033] III. Adding nutrients to the porous cellulose pellet mixture solution obtained in step II to obtain a cellulose-based carrier;

[0034] 2. Bacillus load

[0035] Mix 100 parts by weight of the cellulose-based carrier with 8-12 parts by weight of Bacillus, add culture solution, stir evenly to obtain a mixed liquid, and send it to an incubator for aerated culture. Preferably, the dissolved oxygen content is 2-4mg / L,...

Embodiment 1

[0046] 1. Preparation of cellulose-based carrier

[0047] Make a mixed solution of NaOH, urea and water according to the mass ratio of 3:4:80, add 10% mass of crushed sawdust, freeze at 0°C-5°C for 10 hours, and stir while thawing to obtain a cellulose solution ; Add 5% CaCO to the cellulose solution 3 Ultrasonic 30 minutes, obtain porous cellulose bead mixed solution; Add 5% glucose of mixed solution weight, 0.1% ammonium sulfide, 0.25% potassium dihydrogen phosphate, 0.05% Disodium hydrogen phosphate, 0.1% magnesium sulfate heptahydrate, 0.01% ferric sulfate heptahydrate and 0.05% yeast extract were used to prepare the cellulose-based carrier.

[0048] 2. Bacillus load

[0049] Mix 100 grams of cellulose-based carrier and 10 grams of Bacillus, add 100 ml of culture solution, the composition of the culture solution is: 0.564 grams of NH in 1L solution 4 HCO 3 , 0.380 g MgSO 4 ·7H 2 O, 0.02g CaCl 2 2H 2 O, 0.087gK 2 HPO 4 , and stir evenly to obtain a mixed liquid, w...

Embodiment 2

[0054] 1. Preparation of cellulose-based carrier

[0055] Make a mixed solution of NaOH, urea and water according to the mass ratio of 5:5:100, add 15% mass of crushed straw, freeze at 0°C-5°C for 6 hours, and stir while thawing to obtain a cellulose solution ; Add 10% CaCO to the cellulose solution 3 Ultrasonic 60 minutes, obtain porous cellulose bead mixed solution; Add 5% glucose of mixed solution weight, 0.1% ammonium sulfide, 0.25% potassium dihydrogen phosphate, 0.05% Disodium hydrogen phosphate, 0.1% magnesium sulfate heptahydrate, 0.01% ferric sulfate heptahydrate and 0.05% yeast extract were used to prepare the cellulose-based carrier.

[0056] 2. Bacillus load

[0057] Mix 100 grams of cellulose-based carrier and 8 grams of Bacillus, add 90 ml of culture solution, the composition of the culture solution is: 0.564 grams of NH in 1L solution 4 HCO 3 , 0.380 g MgSO 4 ·7H 2 O, 0.02g CaCl 2 2H 2 O, 0.087gK 2 HPO 4 , and stir evenly to obtain a mixed liquid, whic...

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Abstract

The invention discloses a preparation method of a microbial agent for repairing cadmium-contaminated soil. The method comprises the following steps: mixing NaOH, urea and water to obtain a mixed solution according to a mass ratio of (3-7):(3-5):(80-100), adding 10%-20% by mass of cellulose, performing storing for 6-12 hours at the temperature of 0-5 DEG C to obtain a cellulose solution, adding 5%-10% of CaCO3, and performing ultrasonic treatment to obtain a porous cellulose pellet mixed solution; adding nutrient substances into the mixed solution to obtain a cellulose-based carrier; and mixing100 parts by weight of the cellulose-based carrier with 8-12 parts by weight of bacillus, adding a culture solution, performing uniform stirring to obtain a mixed solution, and feeding the mixed solution into an incubator for aeration culture so as to obtain the microbial agent. The microbial agent prepared by the invention can repair the heavy metal Cd (II) in a targeted manner, and is wide in raw material source, low in price, environmentally friendly, simple in preparation method, low in equipment requirement and convenient for large-scale production.

Description

technical field [0001] The invention belongs to the technical field of soil remediation. Specifically, the invention relates to a microbial agent for repairing cadmium-contaminated soil. In particular, the invention also relates to a preparation method and application of the microbial agent. Background technique [0002] Soil polluted by heavy metals is generally treated by physical and chemical methods. These treatment methods are usually costly and can easily lead to soil structure damage and affect microbial flora, resulting in secondary pollution. Although phytoremediation technology avoids the pollution problems in the process of physical remediation and chemical remediation, it cannot be widely promoted due to the high cost of treatment and the occupation of land resources. [0003] Microbial remediation of cadmium-contaminated soil technology is to use the transformation and immobilization of microorganisms to change the form of cadmium in the soil, thereby changing i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12N11/10C12N11/04B09C1/10C12R1/07
CPCC12N11/12C12N11/10C12N11/04B09C1/10
Inventor 杨燕群黄志红黄迪魏东升龙翔
Owner YUNNAN SHENGQING ENVIRONMENTAL PROTECTION TECH CO LTD
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