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Individualized intervention teabag for gene of high blood lipid and preparation method of individualized intervention teabag

A technology for making tea in bags and hyperlipidemia, which is applied in biochemical equipment and methods, tea substitutes, microbiological determination/inspection, etc., and can solve problems such as toxicity

Inactive Publication Date: 2020-07-07
上海利康精准医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the food industry mainly uses artificially synthesized antioxidants, such as BHT, BHA, etc. Although they have many advantages, they also have different degrees of toxicity

Method used

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  • Individualized intervention teabag for gene of high blood lipid and preparation method of individualized intervention teabag
  • Individualized intervention teabag for gene of high blood lipid and preparation method of individualized intervention teabag
  • Individualized intervention teabag for gene of high blood lipid and preparation method of individualized intervention teabag

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Detection of Gene Variation

[0040] Individual oral epithelial cell samples were collected, and genomic DNA was extracted by silica gel adsorption method. After electrophoresis detection and verification, fluorescent quantitative PCR reaction was performed.

[0041] Individual samples were put into three reaction wells, and three sites were detected simultaneously, namely, rs1801133 of the MTHFR gene, rs285 of the LPL gene, and rs2230806 of the ABCA1 gene. In addition, NTC blank control wells without DNA templates were added according to the experimental needs;

[0042] Add reagents to each reaction well as a fluorescent quantitative PCR reaction system with a total volume of 10 μL, that is, 2 μL of DNA template with a concentration of 20 ng / μL, 10× fluorescent quantitative PCR reaction buffer ABITaqman ® MGB 1 μL, 25mM dNTP four deoxynucleotide substrates for DNA synthesis 0.1 μL, 25mM MgCl 2 Solution 0.5μL, (5units / μL) TaqDNA polymerase 0.02μL, deionized ...

Embodiment 2

[0047] Example 2 Assessing the Risk of Variation in the Detected Gene

[0048] According to the relevant research results of molecular epidemiology in a large-scale Chinese population, the risk of different genotypes of the three loci measured in the embodiment is shown in Table 2 below:

[0049] Table 2

[0050]

[0051] Each polymorphic locus associated with hyperlipidemia has three subtypes, and the possible permutations and combinations of all subtypes of the selected loci are combined; then the risks of these subtypes are multiplied to obtain the risk of different permutations and combinations Degree product; Arrange the obtained risk products from small to large or from large to small, and merge groups with equal risk products; finally determine the level of risk of each group according to the size of the risk product.

[0052] The above method weights each site as an independent factor. The greater the risk product, the greater the risk assessment; There are more g...

Embodiment 3

[0061] Example 3 Preparation and consumption of individualized intervention teabags

[0062] (1) Raw material preparation

[0063] The relevant raw materials were prepared according to the data in Table 5 below, in which various components came from the Zhonghetang Clinic.

[0064] Table 5 Individualized intervention teabag formula

[0065]

[0066] According to the proportions in Table 6 below, the relevant formula compositions in Table 5 were selected to prepare full formula individualized intervention teabags and non-full formula individualized intervention teabags.

[0067] Table 6

[0068]

[0069] (2) Production of various intervention teabags

[0070] The production of individualized intervention teabags can be completed through a fully automated low-temperature teabag preparation system.

[0071] Preferably, the preparation unit of the fully automated low-temperature teabag preparation system includes an automatic sampling device 1, a microwave sterilizing comp...

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Abstract

The invention provides an individualized intervention teabag for the gene of high blood lipid and a preparation method of the individualized intervention teabag. The individualized intervention teabagis prepared by using the following steps: 1) determining high-blood-lipid-related gene polymorphic sites with an intervention effect on nutrients; 2) detecting variation of individual MTHFR genes, LPL genes and ABCA1 genes; 3) evaluating the risk of the detected gene variation; and 4) determining the addition amount of different components in the individualized intervention teabag, and preparingthe corresponding individualized intervention teabag through a full-automation low-temperature teabag preparation system. Through the individualized intervention teabag for the gene of high blood lipid, the level of blood lipid of a patient with high blood lipid can be reduced effectively.

Description

technical field [0001] The invention relates to an individualized intervention teabag, in particular to a gene individualized intervention teabag for hyperlipidemia and a preparation method thereof. Background technique [0002] Blood lipid is the general term for lipids contained in the blood, mainly including cholesterol, triglycerides, phospholipids, fatty acids, etc. A large number of research data show that hyperlipidemia is the main risk factor for human atherosclerotic diseases, such as common atherosclerotic diseases are: coronary heart disease (including myocardial infarction, angina pectoris and sudden death), cerebral infarction and peripheral vascular thrombosis embolic disease. The incidence of these cardiovascular and cerebrovascular diseases is high, the harm is great, and the progress of the disease is dangerous. The mortality rate accounts for about half of the total human mortality. According to the survey, the awareness rate of dyslipidemia in the Chines...

Claims

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Application Information

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IPC IPC(8): A23F3/34C12Q1/6883
CPCA23F3/34C12Q1/6883C12Q2600/156
Inventor 陆婧毛丹丹傅咏南
Owner 上海利康精准医疗技术有限公司
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