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Method for extracting Acanthopanax senticosus total saponins from Acanthopanax senticosus leaves

A technology of Acanthopanax leaves and Acanthopanax senticosus, applied in medical formula, non-central analgesics, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2020-07-07
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above methods can all achieve the purpose of purification, a single method has shortcomings and deficiencies

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for extracting total saponins of Acanthopanax senticosus from leaves of Acanthopanax senticosus, carried out according to the following steps:

[0035] 1) Put the dried Acanthopanax senticosus leaves into a mortar and grind them fully, pass through a 100-mesh sieve, and then repeatedly grind the powder with a particle size greater than 100 mesh until all of them pass through the 100-mesh sieve to obtain the leaf powder;

[0036] 2) Ultrasonic extraction: Take 10 g of the leaf powder obtained in step 1 and mix them evenly with 250 mL, 85 wt.% ethanol aqueous solution. After ultrasonication for 10 minutes, obtain the first extract by vacuum filtration, add 250 mL of ethanol aqueous solution to the remaining residue and repeat The above steps and mixing the obtained extract with the first extract;

[0037] 3) Decolorization: Concentrate the mixed extract in step 2 to 75ml, add an appropriate amount of activated carbon, heat in a water bath at 60°C for 10 minutes, ...

Embodiment 2

[0045] A method for extracting total saponins of Acanthopanax senticosus from leaves of Acanthopanax senticosus, carried out according to the following steps:

[0046] 1) Put the dried Acanthopanax senticosus leaves into a mortar and grind them thoroughly, pass through a 150-mesh sieve, and then repeatedly grind the powder with a particle size greater than 150 mesh until all of them pass through the 150-mesh sieve to obtain the leaf powder;

[0047] 2) Ultrasonic extraction: take 15g of the leaf powder obtained in step 1 and mix them with 300mL, 85wt.% ethanol aqueous solution, and after ultrasonication for 15min, obtain the first extract by vacuum filtration, add 300mL ethanol aqueous solution to the remaining residue and repeat The above steps and mixing the obtained extract with the first extract;

[0048] 3) Decolorization: Concentrate the mixed extract in step 2 to 80ml, add an appropriate amount of activated carbon, heat in a water bath at 60°C for 12 minutes, and obtain...

Embodiment 3

[0056] A method for extracting total saponins of Acanthopanax senticosus from leaves of Acanthopanax senticosus, carried out according to the following steps:

[0057] 1) Put the dried Acanthopanax senticosus leaves into a mortar and grind them fully, pass through a 200-mesh sieve, and then repeatedly grind the powder with a particle size greater than 200 mesh until all of them pass through the 200-mesh sieve to obtain the leaf powder;

[0058] 2) Ultrasonic extraction: take 10 g of the leaf powder obtained in step 1 and mix them evenly with 300 mL, 85 wt.% ethanol aqueous solution. After 20 minutes of ultrasonication, filter under reduced pressure to obtain the first extract, add 300 mL of ethanol aqueous solution to the remaining residue and repeat The above steps are performed twice and the obtained extract is mixed with the extract for the first time;

[0059] 3) Decolorization: Concentrate the mixed extract in step 2) to 100ml, add an appropriate amount of activated carbo...

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Abstract

The invention discloses a method for extracting Acanthopanax senticosus total saponins from Acanthopanax senticosus leaves. A crude extraction product obtained by ultrasonic extraction is purified through a combination method of chemical extraction and macroporous resin adsorption, the operation is simple, the extraction efficiency is high, and both an extraction reagent and macroporous resin canbe recycled; organic reagent ethanol is added into a solvent during ultrasonic extraction, the yield of saponins is improved, water-soluble impurities are reduced, and the difficulty of later purification is reduced; ethanol is used as an eluent during macroporous resin elution, the test toxicity is reduced, the purity of an extract is improved, ethanol is an inexpensive extraction reagent, safe and pollution-free and can be recycled during the concentration process, and saving of cost is facilitated; and the Acanthopanax senticosus total saponins are prepared by using a low-temperature and low-pressure freeze-drying method, so that the loss in the preparation process is reduced.

Description

technical field [0001] The invention belongs to the technical field of extracting saponins, and in particular relates to a method for extracting total saponins of Acanthopanax senticosus from leaves. Background technique [0002] Acanthopanax is a kind of Acanthopanax genus of Araliaceae. Its medicinal properties are mild and non-toxic. It has the functions of nourishing and strengthening, nourishing qi and invigorating the spleen, strengthening the body, calming and calming the nerves. It is an immune enhancer and can regulate the metabolism of the human body. , enhance the body's resistance to various diseases, mainly distributed in Northeast my country and North China, and has a long history of being widely used as a drug in Chinese medicine. Modern medical research has proved that Acanthopanax has anti-inflammatory, anti-tumor, anti-radiation, anti-aging, immune regulation and other effects. Because of its wide range of physiological activities, it has attracted the atte...

Claims

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Application Information

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IPC IPC(8): A61K36/254A61P29/00A61P35/00A61P39/00A61P39/06A61P37/02A61P37/04A61K127/00
CPCA61K36/254A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P29/00A61P35/00A61P37/02A61P37/04A61P39/00A61P39/06
Inventor 王卓邢朝斌龙月红
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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