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A compound antagonistic agent for preventing and controlling tobacco diseases and its application

An antagonistic agent and antagonistic agent technology, applied in the field of compound antagonistic agent for the prevention and control of tobacco diseases, can solve the problems of loss of biological control function, reduction, difficulty in colonization and propagation of antagonistic microorganisms, etc.

Active Publication Date: 2021-06-25
HUBEI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, for the prevention and control of these two types of diseases, the project team has carried out the research and development of microbial biocontrol agents in the early stage, screened and obtained some antagonistic bacteria, and achieved certain control effects. However, due to the complexity of the soil and ecological environment, many antagonistic microorganisms It is difficult to colonize and multiply in the field, thus reducing or even losing the proper biological control function

Method used

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  • A compound antagonistic agent for preventing and controlling tobacco diseases and its application
  • A compound antagonistic agent for preventing and controlling tobacco diseases and its application
  • A compound antagonistic agent for preventing and controlling tobacco diseases and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Effect of Example 1 Syramidic Antagonistic Fungicide on the Germination of Tobacco Seeds

[0042] The increased effect antagonist filtrate was set to 100%, 75%, 50%, 25% and 0% 5 mass volume specific concentration gradient, and 10 ml soaked in tomato and tobacco seeds were taken for 72 hours, and then placed in the light incubator culture, Seed germination testing, each process is three repetitions, with a bud length as an indicator, with a 0% concentration of control, and calculate the promotion rate or inhibition of the crop seed germination by the formula.

[0043] Promotional rate (inhibition rate)% = (Treatment of a bud length average - control bud length average) / control group bud length average × 100%.

[0044] The results are as follows and figure 1 As shown, the increase in antagonist agent has a certain promotion effect on seed germination, administering 100%, 75%, 50%, and 25% concentrations of deterioration of the seed germination promotion rate of seed germin...

Embodiment 2

[0052] Example 2 Syramidic antagonist agent in serum disease prevention, black tibia prevention and treatment of tobacco plant growth

[0053] The tobacco variety involved in this embodiment: cloud smoke 87; soil: Enshi Xi'an. The groups involved in the present invention include: CK: No treatment; T1: Synergistic binder (composite antagonistic spacium: chitosan: marigold water): amino oligo olean = 1: 1: 1: 1 ); T2: Composite antagonistic bacteria (ZM9: DH9: ZH2: YH-22 = 1: 1: 1: 1); T3: Synergic ingredients (chitosan: Marigold: Amino oligoconan = 1: 1: 1 ).

[0054] Test analysis index and method according to the embodiment of the present invention

[0055] (1) Changes in the abundance in soil: 1 g of the hyperviliation of antagonistic bacteria, composite antagonistic bacteria, and the mixing composition, 100ml of water, and 30ml water and movable 15 days after the smoke seedlings, after 30 days of taking 1g after different treatment, 50 ml of water, add 100 μl of soil suspension...

Embodiment 3

[0079] Example 3 Operating Antagonistic Fungi Domada Application Evaluation

[0080] 1, experimental method

[0081] The experimental location of this embodiment: the field test is set in the cooling zone of the Xiongan Pioni Garden.

[0082] Group test design:

[0083] Set a large regional test, not using a fungicide during the full growth period of the pipe straam; other field management is carried out in convention.

[0084] CK: Control, non-administration of fungicides and synergistic components.

[0085] T1: Synergistic antagonistic bacteria and rice bran according to the 1: 1 ratio, after the fermentation of 3 days, mixed with the cultivation in accordance with 1: 1, in the non-group, 30 ~ 40D after the smoke seeds The use of 300 mg / strain is applied.

[0086] T2: Composite Aglicte and Rice Fad After 1: 1 Compare, Fermentation 3 days, with the cultivation of the dizzlor mixed with the pelicine, in the smoke seedling plant, 30 ~ 40D after the transplantation, the amount of ...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a compound antagonistic antibacterial agent for preventing and controlling tobacco diseases and its application. Based on the disease-resistant substances discovered earlier and the screened antagonistic bacteria, the invention fully exerts the effect of disease-resistant proliferation and realizes the simplification of prevention and control technology through the development of "synthetic antagonistic bacteria", providing technical support for the development of tobacco leaf production. The study found that the developed synergistic antagonistic agent can promote the germination and growth of tobacco seeds; under potted conditions, the synergistic antagonistic agent can significantly enhance the control effect of the soil-borne disease tobacco bacterial wilt, and the application of synergistic antagonistic agent The relative control effect of the group on bacterial wilt was 60.87%; the relative control effect on black shank was 60.32%. According to the data analysis of maximum leaf length, maximum leaf width, plant height and stem diameter, synergistic antagonistic antibiotics can significantly promote the growth of tobacco plants.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and more particularly to a composite antagonistic agent for preventing control of tobacco disease and its application. Background technique [0002] Due to the more consumer of tobacco variety in Hubei Province, the trend of genetic homogenization is increasing, resulting in an increase in the choice of pathologies, and constantly produces a stronger pathogen or small species; excessive administration of fertilizers and pesticides, Yigi Soil In addition to the reduction in microorganisms, the ecological diversity is seriously damaged, providing pathogens to the host and reproductive places of survival, the secondary disease is gradually rising as the main disease, and the phenomenon of composite infection is increasingly serious. The emergence of the above problems caused the native disease, and the star disease has become the main tobacco disease in Wuling Qinba ecological area. [0003] At prese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01N43/16A01N65/12A01P3/00A01P1/00A01P21/00C09K17/14
CPCA01N43/16A01N63/00A01N65/12C09K17/14
Inventor 杨勇黎妍妍陈守文李程杨小琼李春黎马昕蔡冬波王勤
Owner HUBEI UNIV