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Polypeptide preloaded injectable self-repairing antibacterial hydrogel dressing and preparation method thereof

A hydrogel, self-healing technology, applied in the field of medical materials and its preparation, can solve problems such as unfavorable wound repair, lack of self-healing ability and damage of injectable hydrogels, and achieve excellent biocompatibility and biodegradability. , accelerate wound healing, improve safety effect

Active Publication Date: 2020-07-10
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During clinical treatment, especially for irregular wound sites and deep wound sites, conventional injectable hydrogels are easily damaged by external forces due to the lack of self-healing ability, which is not conducive to wound repair

Method used

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  • Polypeptide preloaded injectable self-repairing antibacterial hydrogel dressing and preparation method thereof
  • Polypeptide preloaded injectable self-repairing antibacterial hydrogel dressing and preparation method thereof
  • Polypeptide preloaded injectable self-repairing antibacterial hydrogel dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of an injectable self-healing antibacterial hydrogel dressing preloaded with polypeptide:

[0046] a) Preparation of aldylated dextran:

[0047] At room temperature, the number average molecular weight of 10000 dextran ( figure 1Structural formula 1) (6g) was dissolved in 100mL of deionized water, sodium periodate (2g) was dissolved in 5mL of deionized water, sodium periodate solution was added dropwise in the dextran solution, and reacted under dark conditions 6 hours, add ethylene glycol (2g) room temperature reaction 1 hour again, gained product ( figure 1 Structural formula 2) was dialyzed for one week, freeze-dried, and stored at low temperature.

[0048] b) Preparation of modified dextran:

[0049] Under room temperature conditions, formaldehyde dextran (1g) was dissolved in deionized water, dodecylamine (0.1g) was dissolved in ethanol solution, in formaldehyde dextran ( figure 1 In structural formula 2) solution, add dodecylamine ( figu...

Embodiment 2

[0066] A preparation method of an injectable self-repairing antibacterial hydrogel dressing preloaded with polypeptides differs from Example 1 in that:

[0067] Described step a) is:

[0068] a) Preparation of aldylated dextran:

[0069] Under room temperature conditions, the number average molecular weight is 50000 dextran ( figure 1 Structural formula 1) (6g) was dissolved in 100mL of deionized water, sodium periodate (2g) was dissolved in 5mL of deionized water, sodium periodate solution was added dropwise in the dextran solution, and reacted under dark conditions 6 hours, add ethylene glycol (2g) room temperature reaction 1 hour again, gained product ( figure 1 Structural formula 2) was dialyzed for one week, freeze-dried, and stored at low temperature.

[0070] b) Preparation of modified dextran:

[0071] Under room temperature conditions, formaldehyde dextran (1g) was dissolved in deionized water, dodecylamine (0.1g) was dissolved in ethanol solution, in formaldehyde...

Embodiment 3

[0073] A preparation method of an injectable self-repairing antibacterial hydrogel dressing preloaded with polypeptides differs from Example 1 in that:

[0074] Described step a) is:

[0075] a) Preparation of aldylated dextran:

[0076] Under room temperature conditions, the number average molecular weight is 100000 dextran ( figure 1 Structural formula 1) (6g) was dissolved in 100mL of deionized water, sodium periodate (2g) was dissolved in 5mL of deionized water, sodium periodate solution was added dropwise in the dextran solution, and reacted under dark conditions 6 hours, add ethylene glycol (2g) room temperature reaction 1 hour again, gained product ( figure 1 Structural formula 2) was dialyzed for one week, freeze-dried, and stored at low temperature.

[0077] b) Preparation of modified dextran:

[0078] Under room temperature conditions, formaldehyde dextran (1g) was dissolved in deionized water, dodecylamine (0.1g) was dissolved in ethanol solution, in formaldehyd...

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Abstract

The invention discloses a polypeptide preloaded injectable self-repairing antibacterial hydrogel dressing and a preparation method thereof. The hydrogel dressing comprises a hydrogel combined with a fabric, wherein polypeptides for promoting migration of fibroblasts are distributed on the surface of the hydrogel and in the hydrogel. The preparation method comprises the following steps: selectivelyoxidizing glucans with different molecular weights into aldehyde glucans through an oxidizing agent; performing reacting on the aldehyde glucans with aliphatic amine to obtain partially alkylated glucans; then preparing the polypeptides through solid phase; and respectively dissolving the aldehyde glucans, adipic dihydrazide and the partially alkylated glucans into PBS buffer solutions, adding the polypeptides into the aldehyde glucan solution to preload the polypeptides synthesized through solid phase, mixing the three solutions according to a certain mass ratio to prepare the polypeptide preloaded injectable self-repairing antibacterial hydrogel, and finally preparing the dressing. The hydrogel dressing prepared by the method has good antibacterial performance, can reduce the chance ofwound infection, has dynamic self-repairing performance, can aim at irregular wounds, can keep the wound surface moist, and can accelerate wound healing.

Description

technical field [0001] The invention relates to an injectable dynamic self-repairing antibacterial hydrogel dressing preloaded with polypeptide and a preparation method thereof, belonging to the technical field of medical materials and its preparation. Background technique [0002] Wound healing is a complex and coordinated process that includes hemostasis, inflammation, cell proliferation and extracellular matrix remodeling. Currently, bacterial infection and poor wound healing remain major challenges in wound management. Bacterial infection often leads to the formation of exudate, which greatly hinders wound healing. In addition, chronic and deep wounds are often difficult to angiogenesis and self-repair due to reduced activity of endogenous growth factors. To overcome these problems, many wound dressing materials such as films, hydrogels, foams, and hydrocolloids have been developed to meet the requirements of preventing infection and accelerating wound closure. Among ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/32A61L15/42A61L15/46A61L15/44A61L15/62A61L26/00C07K14/47C07K1/06C07K1/04
CPCA61L15/28A61L15/32A61L15/42A61L15/44A61L15/46A61L15/62A61L26/0023A61L26/0047A61L26/0066A61L26/008A61L26/009A61L2300/232A61L2300/252A61L2300/404A61L2300/412A61L2400/06C07K14/47C08L5/02C08L89/00Y02P20/55
Inventor 吴德群叶婷婷刘畅王学利李发学俞建勇
Owner DONGHUA UNIV
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