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Method for identifying corn bacterial stripe disease caused by Pantoea ananatis

A technology of Pantoea pineapple and identification method, which is applied in the direction of biochemical equipment and methods, microbe measurement/inspection, etc., can solve the problems of sweet corn hazards, etc., achieve good repeatability, shorten the identification time, and shorten the time from inoculation to onset Effect

Pending Publication Date: 2020-07-14
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its variety characteristics and high sugar content, sweet corn is harmed by various microorganisms in the field

Method used

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  • Method for identifying corn bacterial stripe disease caused by Pantoea ananatis
  • Method for identifying corn bacterial stripe disease caused by Pantoea ananatis
  • Method for identifying corn bacterial stripe disease caused by Pantoea ananatis

Examples

Experimental program
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Effect test

preparation example Construction

[0041] The preparation method of LB liquid medium is: 10g Tryptone (Tryptone), 5g Yeastextract (Yeasextract), 5g NaCl, NaOH to adjust the pH to 7.4, and dilute to 1L with deionized water. Steam sterilization under high pressure for 20min.

[0042]The preparation method of LB solid medium is: 10g Tryptone (Tryptone), 5g Yeastextract (Yeasextract), 5g NaCl, 15g agar, adjust pH to 7.4 with NaOH, and dilute to 1L with deionized water. Steam sterilize under high pressure for 20min, pour into 10cm Petri dish.

[0043] Field-affected strains of bacterial streak disease, such as figure 1 shown.

[0044] The following biological materials are involved in the examples:

[0045] Pantoea ananatis was purchased from China General Microorganism Culture Collection and Management Center, CGMCC: 1.15633. The result graph of Pantoea pineapple cultured on LB solid medium, such as figure 2 shown.

[0046] Yuetian No. 28, approval number: Yueshenyu 20170004, can also be obtained from the Cr...

Embodiment 1

[0049] Example 1: Identification of resistance to bacterial stripe disease of sweet corn variety Yuetian 28

[0050] 1. Cultivation of inoculated seedlings of sweet corn

[0051] (1) Take 40 seeds of Yuetian No. 28 in ddH 2 Soak in O at 28°C for 16h. The seeds after imbibition were soaked with 75% alcohol for 5 minutes to carry out surface disinfection, and then the seeds were sterilized with ddH 2 O was washed three times; after cleaning, the seeds were soaked in 10% NaClO for 30 min, disinfected again, and then sterilized with ddH 2 O washed three times.

[0052] (2) Seeds are sown in nutrient soil, using small flowerpots with a diameter of 10 cm, and 4 seeds are sown in each flowerpot. Place the sown pots in the light incubator. The environment of the incubator was set at 28° C., humidity 60%, light time 16 hours, light intensity 30000 Lx, dark time 8 hours, light intensity 0 Lx. The corn seedlings were cultivated to the stage of three leaves and one heart.

[0053] ...

Embodiment 2

[0067] Example 2: Identification of resistance to bacterial stripe disease of sweet corn inbred line group 1-1

[0068] 1. Cultivation of inoculated seedlings of sweet corn

[0069] (1) Take 40 seeds of group 1-1 in ddH 2 Soak in O at 28°C for 16h. The seeds after imbibition were soaked with 75% alcohol for 5 minutes to carry out surface disinfection, and then the seeds were sterilized with ddH 2 O was washed three times; after cleaning, the seeds were soaked in 10% NaClO for 30 min, disinfected again, and then sterilized with ddH 2 O washed three times.

[0070] (2) Seeds are sown in nutrient soil, using small flowerpots with a diameter of 10 cm, and 4 seeds are sown in each flowerpot. Place the sown pots in the light incubator. The environment of the incubator was set at 28° C., humidity 60%, light time 16 hours, light intensity 30000 Lx, dark time 8 hours, light intensity 0 Lx. The corn seedlings were cultivated to the stage of three leaves and one heart.

[0071] 2....

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Abstract

The invention discloses a method for identifying corn bacterial stripe disease caused by Pantoea ananatis, and relates to the technical field of crop disease-resistant breeding. The method includes the following steps of corn seedling cultivating, pathogenic bacteria extending propagation, pathogenic bacteria inoculation and resistance identification. The inoculation is carried out by using a needle of a syringe to draw a line along the veins of corn leaves to create wounds so as to assist the invasion of pathogenic bacteria, and a pathogenetic environment is simulated, so that Pantoea ananatis can successfully develop on the corn leaves. The identification method is simple to operate and can be completed indoors, the repeatability is good, and the efficiency is high; and the identification method is not affected by seasons and external environments, the number and growth status of pathogenic bacteria are ensured, precise inoculation and identification are achieved, resistance identification can be carried out on corn materials in a short period of time, the identification time is greatly shortened, the rapid identification of the bacterial stripe disease in corn inbred lines is facilitated, and the application value is great.

Description

technical field [0001] The invention relates to the technical field of crop disease-resistant breeding, in particular to an identification method for corn bacterial stripe disease caused by Pantoea pineapple. Background technique [0002] Sweet corn originated in the Americas and is a subspecies of the maize species controlled by one or more natural recessive mutation genes in the endosperm starch synthesis pathway. Sweet corn has the characteristics of grain, fruit and vegetable. It not only has unique flavor and good palatability, but also contains high protein, amino acids, vitamins and minerals, and has high nutritional value (Zhang Ying, Li Shiqun, Mu Nan, etc. fresh Research and development status and development prospects of food-type corn [J]. Miscellaneous Grain Crops, 2007,27(5):373~375.). With the growth of the national economy and the adjustment of consumers' dietary structure, the planting area and demand of sweet corn in my country are also increasing. Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 张楠李高科胡建广于永涛李武谢利华李光玉
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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