Neonatal blood type and hemolytic disease comprehensive detection card

Abstract

The invention discloses a neonatal blood type and hemolytic disease comprehensive detection card, which comprises a blood type identification area and a hemolysis three-item detection area, wherein the blood type identification area comprises an ABO and RhD blood type detection test strip, and the test strip is sequentially provided with a washing area, a sample adding reaction area and a water absorption area from upstream to downstream. The neonatal blood type and hemolytic disease comprehensive detection card is characterized in that the sample adding reaction area is provided with a detection point A, a detection point B and a detection point D, and ABO and RhD blood type identification can be simultaneously completed by one drop of sample; the hemolysis three-item detection area comprises a neonatal erythrocyte direct antiglobulin test (DAT) detection part, a neonatal free antibody detection part and a neonatal erythrocyte diffusion liquid antibody detection part which are sequentially arranged side by side; each detection part comprises a same test strip; the test strip is sequentially provided with a washing area, a sample adding area, a reaction area and a water absorptionarea from upstream to downstream; the reaction area is provided with an IgG anti-A detection point and an IgG anti-B detection point, and whether neonatal serum has an immune blood group antibody froma mother or not can be ensured to be specifically detected, so that a sensitization red blood cell washing step of a conventional liquid reagent is not needed, and the sensitivity is higher. The neonatal blood type and hemolytic disease comprehensive detection card has the advantages of room-temperature preservation, no need of auxiliary equipment, convenience in operation, small required sampleamount, short detection time, easiness in result interpretation, difficulty in causing pollution, high specificity and sensitivity and the like, and is suitable for rapid bedside detection of neonatalblood types and hemolytic diseases.

Description

technical field [0001] The invention belongs to the technical field of blood detection, and in particular relates to a comprehensive detection card for neonatal blood type and hemolytic disease. Background technique [0002] Hemolytic disease of the newborn (HDN) is one of the most common acute hemolytic diseases in pediatrics. IgG antibody is the main type, and this IgG antibody can enter the fetus through the placenta, destroying fetal red blood cells and causing HDN. In severe cases, miscarriage, premature delivery, stillbirth, hemolytic jaundice, severe anemia, and nuclear jaundice may develop. It can cause early death of infants or leave survivors with irreversible neurological sequelae, bringing great pain to children and their families. Among the human blood group systems discovered so far, hemolytic disease of the newborn caused by ABO blood group incompatibility is the most common, followed by the Rh blood group system, and HDN caused by other blood group systems i...

AI Technical Summary

Problems solved by technology

It has many shortcomings: 1) Liquid reagents need to be refrigerated and cold chain transported, which is not conducive to storage; 2) Samples need to be pre-treated; 3) Additional centrifuge equipment is needed; 4) Test tubes need to be shaken to interpret results, which is greatly affected by human factors , which is not conducive to the interpretation of the results

It also has many shortcomings: 1) Liquid reagents need to be refrigerated and transported in cold chain, which is not conducive to storage; 2) Additional centrifuge equipment is required; 3) Test tubes need to be shaken to interpret the results, which is greatly affected by human factors, which is not conducive to result interpretation

The micro-column gel method is also used for detection on the market, but there are also shortcomings, such as: high cost, long detection time, and the need for auxiliary equipment, etc.

It also has many shortcomings: 1) liquid reagents need to be refrigerated and cold chain transported, which is not conducive to storage, 2) additional centrifugal equipment is required, 3) long-term sensitization is required, 4) tedious washing of sensitized red blood cells is required , 5) It is necessary to shake the test tube to interpret the results, which is greatly influenced by human factors, which is not conducive to the interpretation of results

Because the determination process is too complicated, large hospitals basically adopt the micro-column gel card method, which can save the steps of red blood cell washing and microscope interpretation, but the whole process is still time-consuming, cumbersome and expensive

Images