Antioxidant peptide, soybean protein hydrolyzate containing the antioxidant peptide
A technology of soybean protein and antioxidant peptides, applied in peptide/protein components, medical preparations containing active ingredients, peptides, etc., can solve the problems of unreported, unreported dipeptides or tripeptides, etc., and achieve high antioxidant activity , easy absorption effect
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[0068] Hereinafter, the present invention will be described more specifically by way of examples.
[0069] (test methods)
[0070] First, various measurement methods used in Examples are summarized below.
[0071]
[0072] The prepared soybean protein hydrolyzate was diluted to 0.1% by weight with ultrapure water, passed through a 0.22 μm filter, and used as a sample for peptide sequence analysis. Peptide sequence analysis was performed by LC-MS / MS analysis. LC-MS / MS analysis was carried out using Agilent 1200 series (manufactured by Agilent Technologies), LTQ Orbitrap XL (manufactured by Thermo Fisher Scientific), and a TOSHO TSKgel ODS-100V column (4.6 mm×250 mm, 5 μm). The MS conditions are: ESI+, spray voltage 4kV, capillary temperature 300°C, and scan analysis for the mass range 100-2000.
[0073]
[0074] 100 mg of the obtained samples were weighed, dissolved in 50% ethanol, and the volume was adjusted to 100 ml, and measured according to the method described in N...
manufacture example 1）
[0077] (Manufacturing example 1) Preparation of isolated soybean protein
[0078] Soy protein isolate was prepared from low modified defatted soybeans as follows.
[0079] (1) Add water to 1 kg of low-modified defatted soybeans, extract the slurry with hot water, and use a centrifuge to remove the bean dregs fraction to obtain defatted soybean milk.
[0080] (2) Adjust the pH of the obtained defatted soybean milk to 4.5, carry out isoelectric point precipitation, and use a centrifuge to obtain acid precipitated casein, and carry out neutralization.
[0081] (3) The obtained fractions were neutralized and heat-sterilized at 120° C. for 10 seconds.
manufacture example 2）
[0082] (Manufacturing example 2) Preparation of soybean protein hydrolyzate A
[0083] A 3% solution of the isolated soybean protein obtained in Production Example 1 was prepared, and metalloprotease "Thermoase" (origin: Bacillus thermoproteolyticus Rokko, Daiwa Kasei Co., Ltd.) was added at 1% or 2% relative to the protein, at pH 9.0, 58 The enzyme reaction was carried out at ℃ for 60 minutes.
[0084] Next, serine protease "Bioprase" (origin: Bacillus sp., Nagase ChemteX Corporation) was added at 0.5% or 1% to the protein, and the enzyme reaction was performed at pH 7.5 and 58°C for 60 minutes.
[0085] Next, metalloprotease "Sumizyme FP" (origin; Aspergillus oryzae, Nippon Chemical Industry Co., Ltd.) was added at 0.5% or 1% to the protein, and the enzyme reaction was carried out at pH 7.5 and 58°C for 60 minutes.
[0086] After the above enzyme reaction, the enzyme reaction liquid was heat-treated at 90° C. for 20 minutes to stop the reaction, and then freeze-dried to obt...
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