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A kind of adipose tissue cryopreservation liquid, preparation method and adipose tissue storage method

An adipose tissue and storage method technology, applied in the fields of adipose tissue cryopreservation, adipose tissue storage, and preparation, can solve problems such as adverse reactions, and achieve the effects of reducing chemical damage, good activity, and maintaining activity

Active Publication Date: 2021-07-23
HEBEI KANGTENG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the above-mentioned prior art has the following defects: first, the cryopreservation solution is based on the basal culture solution, and its composition is uncertain, which may cause certain immunogenicity in it, thereby causing the clinical symptoms of later stage recipients. Adverse reactions; second, about 20% dimethyl sulfoxide is added to the cryopreservation solution, which can better preserve the activity of adipose tissue and the survival rate after transplantation, but dimethyl sulfoxide is cytotoxic, and the added amount exceeds 15 %, the toxic effect will be greater, which increases the risk of transplantation; third, due to the one-step vitrification technology, it needs to be combined with a high-concentration cryopreservation solution to achieve antifreeze protection for adipose tissue, but high-concentration cryopreservation The liquid can cause toxic damage to the cells and devitrified ice crystal damage, which increases the risk of transplantation

Method used

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  • A kind of adipose tissue cryopreservation liquid, preparation method and adipose tissue storage method
  • A kind of adipose tissue cryopreservation liquid, preparation method and adipose tissue storage method
  • A kind of adipose tissue cryopreservation liquid, preparation method and adipose tissue storage method

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preparation example 1

[0041] The human albumin nanoparticles of the present invention are prepared through the following steps:

[0042] Take 3g of egg yolk lecithin, add 40g of absolute ethanol to dissolve to obtain an emulsion; mix 10g of human serum albumin with 20g of dimethyl sulfoxide, stir well to obtain a protein solution; add the emulsion to the protein solution at a speed of 5g / min During the addition process, continue to stir, after the addition, add hydrochloric acid to adjust the pH to 4; use a high-speed homogenizer to perform high-speed homogenization at a stirring speed of 20000r / min, stir for 50min until solidification, and obtain human serum albumin nano Particles: washing and filtering the human serum albumin nanoparticles twice with a phosphate buffer solution with a pH of 6.5-7.0, and finally obtaining the human serum albumin nanoparticles.

preparation example 2

[0044] The human albumin nanoparticles of the present invention are prepared through the following steps:

[0045] Take 3g of egg yolk lecithin, add 40g of absolute ethanol to dissolve to obtain an emulsion; mix 10g of human serum albumin with 20g of dimethyl sulfoxide, stir well to obtain a protein solution; add the emulsion to the protein solution at a speed of 5g / min During the addition process, continue to stir, after the addition, add hydrochloric acid to adjust the pH to 4; use a high-speed homogenizer to perform high-speed homogenization at a stirring speed of 25000r / min, stir for 45min until solidification, and obtain human serum albumin nanoparticles Particles: washing and filtering the human serum albumin nanoparticles twice with a phosphate buffer solution with a pH of 6.5-7.0, and finally obtaining the human serum albumin nanoparticles.

preparation example 3

[0047] The human albumin nanoparticles of the present invention are prepared through the following steps:

[0048] Take 3g of egg yolk lecithin, add 40g of absolute ethanol to dissolve to obtain an emulsion; mix 10g of human serum albumin with 20g of dimethyl sulfoxide, stir well to obtain a protein solution; add the emulsion to the protein solution at a speed of 5g / min During the addition process, continue to stir, after the addition, add hydrochloric acid to adjust the pH to 4; use a high-speed homogenizer to perform high-speed homogenization treatment at a stirring speed of 30000r / min, stir for 40min until solidification, and obtain human serum albumin nanoparticles Particles: washing and filtering the human serum albumin nanoparticles twice with a phosphate buffer solution with a pH of 6.5-7.0, and finally obtaining the human serum albumin nanoparticles.

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Abstract

The invention relates to a fat tissue cryopreservation solution, a preparation method and a fat tissue storage method. The adipose tissue cryopreservation solution is prepared from the following raw materials in percentage by weight: trehalose 2-4%, human albumin nanoparticles 8 -15%, pH 6.5-7.2 phosphate buffer 3-5%, propylene glycol supplemented to 100%, the human albumin nanoparticles are prepared by the following steps: take 3-5 parts by weight of egg yolk lecithin, add 40-50 parts by weight of absolute ethanol is dissolved to obtain an emulsion; 10-15 parts by weight of human albumin is mixed with 20-30 parts by weight of dimethyl sulfoxide, and stirred evenly to obtain a protein solution; the emulsion is added to the protein solution , adjust the pH to 4‑4.5, and stir until solidified to obtain human albumin nanoparticles; adipose tissue preserved with it has good biological activity and transplantation survival rate.

Description

technical field [0001] The invention relates to the technical field of adipose tissue cryopreservation, in particular to an adipose tissue cryopreservation solution, a preparation method and an adipose tissue storage method. Background technique [0002] Autologous fat grafting is a commonly used soft tissue filling method in clinical practice. Adipose tissue has the advantages of abundant sources, simple operation, and no rejection reaction. At present, fat transplantation has been widely used in the field of plastic and cosmetic surgery, such as facial deformities, concave scars, wrinkles, post-traumatic defects, sunken eyelids, skin sunken after liposuction, etc. It is a common technique in the field of beauty or repair. [0003] Although with the deepening of research and the improvement of clinical technology, the survival rate of fat transplantation has been significantly improved, but the biggest problem at present is the necrotic absorption of adipose tissue. Most ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 代金荣刘欣然
Owner HEBEI KANGTENG BIOLOGICAL TECH CO LTD
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