Andrographis paniculata ethyl acetate extract as well as preparation method and application thereof

A technology of ethyl acetate of andrographis paniculata and ethyl acetate of lotus paniculata, which is applied in the field of medicine, can solve problems such as no relevant reports, and achieve the effect of detailed and in-depth physiological activity

Inactive Publication Date: 2020-07-28
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Relevant data at home and abroad can prove that andrographolide is related to hypoglycemic or diabetes, but there are no relevant reports at home and abroad on the relationship between andrographolide extract or andrographolide and GLUT4 activity, GLUT4 expression and the specific mechanism

Method used

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  • Andrographis paniculata ethyl acetate extract as well as preparation method and application thereof
  • Andrographis paniculata ethyl acetate extract as well as preparation method and application thereof
  • Andrographis paniculata ethyl acetate extract as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1 andrographis paniculata ethyl acetate extract and preparation method thereof

[0037]Experimental group: Andrographis paniculata used in the experiment was collected from Bozhou, Anhui, China. Immerse the dried rhizomes of Andrographis paniculata in 80% ethanol solution according to the ratio of material to liquid of 1:5 and stir evenly, stir once every half a day, collect the supernatant after suction filtration every two days, and add 80% ethanol solution to continue soaking . After suction filtration, use a rotary evaporator to evaporate to dryness, and repeat 3-5 times to obtain the ethanol extract of Andrographis paniculata paniculata. Dissolve the ethanol extract with an appropriate amount of ultrapure water, and then first extract it with petroleum ether several times to remove the fat and obtain the petroleum ether part of the drug, and then continue to extract the aqueous phase extract with ethyl acetate. After the ethyl acetate extraction is comp...

Embodiment 2

[0044] Application of embodiment 2 Andrographis paniculata ethyl acetate extract

[0045] 1. The effect of ethyl acetate extract of Andrographis paniculata on the glucose uptake of L6 cells

[0046] 1. Cell glucose uptake experiment: L6 cells were divided into 1×10 4 ~5×10 4 The density was planted in a 96-well plate, and the L6 cells were differentiated for 5 to 7 days with α-MEM culture medium containing 2% FBS, and then the cells to be treated were starved for 2 hours and then added α-mem to prepare a concentration of 60 μg / mL , 90 μg / mL, and 120 μg / mL ethyl acetate extract solutions of Andrographis paniculata were treated with drugs in a 37° C. incubator for 30 minutes. After 30 minutes, take another new 96-well plate to sample according to the instructions of the glucose detection kit. Within 30 minutes after sampling, use a microplate reader to measure the absorbance at a wavelength of 505 to determine the residual glucose. For the number of cells, use an old 96-well ...

Embodiment 3

[0059] Example 3 Ethyl acetate extract of Andrographis paniculata effectively promotes the activity of GLUT4 through AMPK and PKC signaling pathways

[0060] 1. Confocal laser microscopy to detect the effect of trans-signaling pathway inhibitors on GLUT4 translocation: L6 cells stably expressing GLUT4 (GLUT4-mOrange-L6) were used as the research object, and GLUT4-mOrange was used to monitor the translocation of GLUT4 in L6 cells . First, inoculate GLUT4-mOrange-L6 on a glass slide for fluorescence observation. When the cell density is appropriate, starve the cells with serum-free medium for 2 hours, and observe the real-time transport changes of GLUT4-mOrange after adding the drug under a confocal laser microscope. , and detect the fluorescence intensity in the cell, and observe the change of the fluorescence intensity in the cell to reflect the translocation of GLUT4. Group A drugs are: ethyl acetate extract, ethyl acetate extract + Wortmannin; group B drugs are: ethyl aceta...

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Abstract

The invention discloses an andrographis paniculata ethyl acetate extract as well as a preparation method and an application thereof. Experimental research finds that the andrographis paniculata ethylacetate extract has relatively good activity of promoting GLUT4 to transport an upper membrane, and the activity can be inhibited by an AMPK inhibitor Compound C and a PKC inhibitor; and therefore, the andrographis paniculata ethyl acetate extract plays an active role through AMPK and PKC signal pathways. The andrographis paniculata ethyl acetate extract can up-regulate transcription and expression of GLUT4 protein. The invention provides a new research target for the basic research work of GLUT4, AMPK and PKC signal pathways, and is more helpful for carefully and deeply researching the physiological activity of cells.

Description

technical field [0001] The invention belongs to the technical field of medicine, in particular to the application of Andrographis paniculata ethyl acetate extract. Background technique [0002] Type 2 diabetes is also known as non-insulin-dependent diabetes, or adult-onset diabetes. It is due to the decline in the function of insulin to assist glucose into the cell metabolism, the relative lack of insulin secretion, or the decrease in the body's response to insulin, resulting in elevated blood sugar in the body. Type Ⅱ diabetes accounts for about 90% of the total number of diabetic patients, is common in adults over the age of 40, and has obvious family heredity. Most patients are obese, and the onset process is relatively slow and hidden. The symptoms of three excesses and one deficiency (polydipsia, polyuria, polyphagia, and weight loss) may not be obvious, and most patients have no symptoms in daily life. It can only be discovered when other diseases go to the doctor. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/19A61P3/10A61K125/00
CPCA61K36/19A61P3/10A61K2236/333A61K2236/35A61K2236/39
Inventor 赵平杨紫薇
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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