Sperm diluent for donkeys and preparation method and application thereof
A diluent and sperm technology, applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve problems such as insufficient sperm motility, biosafety issues, etc., to maintain sperm cell vitality, reduce electrolyte concentration, and avoid oxidative damage Effect
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Embodiment 1
[0031] Weigh 30.4g of Tris, 17.0g of citric acid, 7.5g of glucose, 12.5mg of taurine, 146.1mg of glutamine, 36.3mg of sodium pyruvate, 7.5mg of glycine, 1.5g of fructose, 2.0g of modified lecithin, Pentoxifylline 1.5g, soybean isoflavones 25.4mg and gentamicin 1000000U. The above-mentioned Tris, citric acid, glucose, taurine and sodium pyruvate were dissolved in 800 mL of water for injection and then filtered through a 0.22 μm filter membrane to remove impurities in the above-mentioned inorganic salt solids. Glutamine, glycine and gentamycin added to the components were stirred and the osmotic pressure of the solution was measured to be 306mOms / kg, and the pH was 7.06. Add modified lecithin, soybean isoflavones and hexanone cocoa to the above solution again After alkali, dilute to 1L, filter through a 0.22μm membrane filter again, pack aseptically in 200mL per bottle, and store in the dark at 2-8°C.
Embodiment 2
[0033] Weigh 29.9g of Tris, 16.8g of citric acid, 7.4g of glucose, 12.3mg of taurine, 145.6mg of glutamine, 35.8mg of sodium pyruvate, 7.4mg of glycine, 1.4g of fructose, 1.9g of modified lecithin, Pentoxifylline 1.4g, soybean isoflavones 25.1mg and gentamicin 1000000U. The above-mentioned Tris, citric acid, glucose, taurine and sodium pyruvate were dissolved in 800 mL of water for injection and then filtered through a 0.22 μm filter membrane to remove impurities in the above-mentioned inorganic salt solids. Glutamine, glycine and gentamycin added to the components were stirred and the osmotic pressure of the solution was measured to be 298mOms / kg, and the pH was 7.14. Add modified lecithin, soybean isoflavones and hexanone cocoa to the above solution again After alkali, dilute to 1L, filter through a 0.22μm membrane filter again, pack aseptically in 200mL per bottle, and store in the dark at 2-8°C.
Embodiment 3
[0035] Weigh 30.9g of Tris, 17.2g of citric acid, 7.6g of glucose, 12.7mg of taurine, 146.6mg of glutamine, 36.8mg of sodium pyruvate, 7.6mg of glycine, 1.6g of fructose, 2.1g of modified lecithin, Pentoxifylline 1.6g, soybean isoflavones 25.7mg and gentamicin 1000000U. The above-mentioned Tris, citric acid, glucose, taurine and sodium pyruvate were dissolved in 800 mL of water for injection and then filtered through a 0.22 μm filter membrane to remove impurities in the above-mentioned inorganic salt solids. Glutamine, glycine and gentamycin added to the components were stirred and the osmotic pressure of the solution was measured to be 302mOms / kg, and the pH was 7.09. Add modified lecithin, soybean isoflavones and hexanone cocoa to the above solution again After alkali, dilute to 1L, filter through a 0.22μm membrane filter again, pack aseptically in 200mL per bottle, and store in the dark at 2-8°C.
[0036] At room temperature of 30°C, three samples were taken in each of the...
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