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Sperm diluent for donkeys and preparation method and application thereof

A diluent and sperm technology, applied in application, preservation of human or animal bodies, animal husbandry, etc., can solve problems such as insufficient sperm motility, biosafety issues, etc., to maintain sperm cell vitality, reduce electrolyte concentration, and avoid oxidative damage Effect

Inactive Publication Date: 2020-08-07
太东(镇江)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention overcomes the defects of the prior art, and proposes a sperm diluent and its preparation method, which solves the biological safety problems caused by the addition of yolk substances in the existing products, and the problem of insufficient sperm viability after dilution

Method used

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  • Sperm diluent for donkeys and preparation method and application thereof
  • Sperm diluent for donkeys and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Weigh 30.4g of Tris, 17.0g of citric acid, 7.5g of glucose, 12.5mg of taurine, 146.1mg of glutamine, 36.3mg of sodium pyruvate, 7.5mg of glycine, 1.5g of fructose, 2.0g of modified lecithin, Pentoxifylline 1.5g, soybean isoflavones 25.4mg and gentamicin 1000000U. The above-mentioned Tris, citric acid, glucose, taurine and sodium pyruvate were dissolved in 800 mL of water for injection and then filtered through a 0.22 μm filter membrane to remove impurities in the above-mentioned inorganic salt solids. Glutamine, glycine and gentamycin added to the components were stirred and the osmotic pressure of the solution was measured to be 306mOms / kg, and the pH was 7.06. Add modified lecithin, soybean isoflavones and hexanone cocoa to the above solution again After alkali, dilute to 1L, filter through a 0.22μm membrane filter again, pack aseptically in 200mL per bottle, and store in the dark at 2-8°C.

Embodiment 2

[0033] Weigh 29.9g of Tris, 16.8g of citric acid, 7.4g of glucose, 12.3mg of taurine, 145.6mg of glutamine, 35.8mg of sodium pyruvate, 7.4mg of glycine, 1.4g of fructose, 1.9g of modified lecithin, Pentoxifylline 1.4g, soybean isoflavones 25.1mg and gentamicin 1000000U. The above-mentioned Tris, citric acid, glucose, taurine and sodium pyruvate were dissolved in 800 mL of water for injection and then filtered through a 0.22 μm filter membrane to remove impurities in the above-mentioned inorganic salt solids. Glutamine, glycine and gentamycin added to the components were stirred and the osmotic pressure of the solution was measured to be 298mOms / kg, and the pH was 7.14. Add modified lecithin, soybean isoflavones and hexanone cocoa to the above solution again After alkali, dilute to 1L, filter through a 0.22μm membrane filter again, pack aseptically in 200mL per bottle, and store in the dark at 2-8°C.

Embodiment 3

[0035] Weigh 30.9g of Tris, 17.2g of citric acid, 7.6g of glucose, 12.7mg of taurine, 146.6mg of glutamine, 36.8mg of sodium pyruvate, 7.6mg of glycine, 1.6g of fructose, 2.1g of modified lecithin, Pentoxifylline 1.6g, soybean isoflavones 25.7mg and gentamicin 1000000U. The above-mentioned Tris, citric acid, glucose, taurine and sodium pyruvate were dissolved in 800 mL of water for injection and then filtered through a 0.22 μm filter membrane to remove impurities in the above-mentioned inorganic salt solids. Glutamine, glycine and gentamycin added to the components were stirred and the osmotic pressure of the solution was measured to be 302mOms / kg, and the pH was 7.09. Add modified lecithin, soybean isoflavones and hexanone cocoa to the above solution again After alkali, dilute to 1L, filter through a 0.22μm membrane filter again, pack aseptically in 200mL per bottle, and store in the dark at 2-8°C.

[0036] At room temperature of 30°C, three samples were taken in each of the...

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Abstract

The invention discloses sperm diluent for donkeys. The invention discloses a donkey sperm diluent and a preparation method thereof. The composition comprises the following components: 30.4+ / -0.5g of tris, 17.0+ / 0.2 g of citric acid; 7.5+ / -0.1 g of glucose, 12.5+ / -0.2 mg of taurine, 36.3+ / -0.5 mg of sodium pyruvate, 1.5+ / -0.1 g of fructose, 2+ / -0.1 g of modified lecithin, 1.5+ / -0.1 g of pentoxifylline and 25.4+ / -0.3 mg of soy isoflavone which are dissolved with water for injection. By adopting the modified lecithin, the damage of irregular particles in common phospholipid to sperms is avoided,the effects of regulating osmotic pressure and protecting sperm cell membranes are achieved, and the survival rate of the sperms is increased; pentoxifylline is adopted, so that the concentration of cAMP in sperm cytoplasm is increased, cAMP dependent protease is activated, tail parts of sperms are phosphorylated, and the athletic ability of the sperms is enhanced; soybean isoflavone is adopted, excessive free oxygen of sperms in an in-vitro environment is removed, oxidative damage to the sperms is avoided, sperm motility is maintained, and the success rate of artificial insemination is increased.

Description

technical field [0001] The invention relates to a donkey sperm diluent and its preparation method and application. Background technique [0002] Donkeys are an important resource for agricultural labor and auxiliary resources for meat, medicine, and leather. However, due to poor breeding efficiency, the number of donkeys has continued to decrease in recent years. Using artificial insemination technology can not only preserve the genetic information resources of excellent male donkeys and improve donkey breeds, but also ensure a high breeding rate while breeding a small number of male donkeys and reducing feeding costs. [0003] In the donkey artificial insemination technique, after obtaining the fresh sperm of the breeding donkey, it is necessary to use a sperm diluent to dilute one part of the original semen into several parts of semen, and then breed multiple female donkeys in estrus. [0004] At present, some sperm diluents on the market have added yolk and other substan...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 刘年
Owner 太东(镇江)生物科技有限公司
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