A kind of rumen-passed enteric-coated antimicrobial peptide granule and its preparation method

An antibacterial peptide and rumen-passing technology, applied in the biological field, can solve the problems of difficulty in passing through the rumen and abomasum, and the antibacterial peptide is easily degraded, and achieves the effects of improving bioavailability, alleviating mastitis, and low production cost.

Active Publication Date: 2022-04-22
安杰利(重庆)生物科技有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current problem that antimicrobial peptides are easily degraded in ruminants and difficult to pass through the rumen and abomasum, the present invention provides a rumen-passing enteric-coated antimicrobial peptide granule, which can pass through the rumen and abomasum of ruminants smoothly, and Fast release in the small intestine for targeted release of antimicrobial peptides in the small intestine of ruminants

Method used

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  • A kind of rumen-passed enteric-coated antimicrobial peptide granule and its preparation method
  • A kind of rumen-passed enteric-coated antimicrobial peptide granule and its preparation method
  • A kind of rumen-passed enteric-coated antimicrobial peptide granule and its preparation method

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Embodiment 1

[0027] 1. Preparation of rumen-passed enteric-coated antimicrobial peptide granules

[0028] 1. Adenosine Heptapeptide Concentrate

[0029] Lactobacillus johnsoni (CGMCC No.18695) fermented liquid, 10 tons of fermenter produces 7 tons of fermented liquid, centrifuged through disc centrifuge (type 250, separation factor 9779, 8600rpm), ceramic membrane (pore size is 50nm~200nm, preferably 200nm) further filter and sterilize to obtain 6.5 tons of supernatant, the content of adenosine heptapeptide is 1%. The supernatant is concentrated to 1 ton through nanofiltration (150Da-400Da, preferably 400Da), and the content of the antibacterial peptide adenosine heptapeptide is 4.0%-5.0%.

[0030] 2. The first layer (inner layer) coating

[0031] Add final concentrations of 0.1% to 0.15% guar gum (high viscosity), 2% to 3% maltodextrin, 2% to 3% starch and 1.5% to 2% sodium alginate to the antimicrobial peptide concentrate, and mix well , forming a suspension, the solution pH=6.5-7.5, ...

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Abstract

The invention relates to a rumen-passing enteric-coated antimicrobial peptide granule. The core material of the antimicrobial peptide is double-coated, the rumen-passing inert fat of the outer layer of packing material isolates the antibacterial peptide from the rumen fluid, and the inner layer of packing material uses calcium alginate Protect the antimicrobial peptides from being destroyed by the low pH value of the abomasum and pepsin, so that the antimicrobial peptides can pass through the rumen and abomasum of ruminants smoothly, and release quickly and at fixed points in the small intestine. Adding the above-mentioned antibacterial peptide granules into the feed can increase animal production, and has the advantages of no residue, no pollution, and the like. The invention also provides a method for preparing rumen-passed enteric-coated antibacterial peptide granules, which can efficiently produce and prepare rumen-passed enteric-coated antibacterial peptide granules, has low production cost, and can be industrially prepared on a large scale.

Description

technical field [0001] The invention relates to a rumen-passing antimicrobial peptide and belongs to the field of biotechnology. [0002] technical background [0003] Antimicrobial peptides are small molecular polypeptides produced by organisms that are related to host defense and innate immunity, generally consisting of 5 to 50 amino acids. Antimicrobial peptides have the functions of inhibiting or killing pathogens, enhancing immunity, promoting wound healing and mucosal defense. For example: adenosine heptapeptide (also known as adenosine heptapeptide, microcin C7 peptide) is a bacteriocin antibacterial peptide secreted by microorganisms such as Enterobacter and Lactobacillus, and is an oligopeptide composed of 7 amino acid residues. , the C-terminus is combined with 5'-adenosine phosphate (AMP) through the N-acylphosphoramide bond to form a small molecular weight antimicrobial peptide, which has strong antibacterial ability and the function of regulating immunity (Ran R...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/08A61K38/16A61K9/50A61K47/36A61P15/14A61P13/04A61P31/04A23K20/147A23K50/10
CPCA61K38/08A61K38/164A61K9/5036A61K9/5015A61P15/14A61P13/04A61P31/04A23K20/147A23K50/10
Inventor 张江杨贤彬
Owner 安杰利(重庆)生物科技有限公司
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