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Method for preparing collagen fiber through wet spinning

A wet spinning and collagen fiber technology, which is applied in the field of biomedical material preparation, can solve the problems of high dissolution temperature, collagen denaturation, ionic liquid toxicity, etc., and achieves the effects of mild conditions, controllable size and green process

Pending Publication Date: 2020-08-07
NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of (PBS) / ethanol mixed solvent can avoid the disadvantages such as the toxicity brought by HFIP to the material, the use of PBS in this system introduces a large amount of salt, which requires dialysis treatment; Low volatility, good solubility, and stable properties have been used, but ionic liquids also have potential toxicity, and the dissolution temperature is too high, resulting in collagen denaturation

Method used

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  • Method for preparing collagen fiber through wet spinning
  • Method for preparing collagen fiber through wet spinning
  • Method for preparing collagen fiber through wet spinning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing collagen fibers by wet spinning, comprising the following steps:

[0028] (1) Dissolving type I collagen in NaAc / HAc buffer solution and stirring continuously at room temperature until type I collagen is completely dissolved to obtain a collagen solution;

[0029] (2) Vacuumizing and defoaming the collagen solution to obtain a collagen spinning solution;

[0030] (3) Wet spinning the collagen spinning solution, and after the fibers obtained by wet spinning are fully shaped, they are stretched and wound to obtain collagen fibers;

[0031] (4) After the above-mentioned collagen fibers are dried at room temperature, the cross-linking treatment is performed, and the above-mentioned cross-linked fibers are dried at room temperature to obtain the finished collagen fibers.

[0032] The coagulation bath used in the wet spinning process in the step (3) is absolute ethanol.

[0033] The cross-linking agent used in the step (4) cross-linking treatment is g...

Embodiment 2

[0041] A method for preparing collagen fibers by wet spinning, comprising the following steps:

[0042] (1) Dissolve type I collagen in NaAc / HAc buffer solution with a pH of 3.0, soak overnight and then stir in a water bath at 25°C until completely dissolved to obtain a collagen solution, in which the concentration of type I collagen is 100mg / mL;

[0043] (2) Filtrating and vacuuming the above-mentioned collagen spinning solution to obtain the collagen spinning solution;

[0044] (3) Add the defoamed collagen spinning solution into the wet spinning experimental device, push the spinning solution out of the needle at a speed of 5mL / h through a syringe pump, and enter it into the coagulation bath with absolute ethanol as the coagulation agent. In the coagulation bath, the fibers obtained by the wet spinning process were fully shaped and then stretched and wound to obtain collagen fibers; wherein, the inner diameter of the syringe needle was 0.08mm, and the spinning temperature w...

Embodiment 3

[0048] A method for preparing collagen fibers by wet spinning, comprising the following steps:

[0049] (1) Dissolve type I collagen in NaAc / HAc buffer solution with a pH of 2.8, and stir in a water bath at room temperature until completely dissolved to obtain a collagen solution, in which the concentration of type I collagen is 50 mg / mL;

[0050] (2) Filtrating and vacuuming the above-mentioned collagen spinning solution to obtain the collagen spinning solution;

[0051](3) Add the defoamed collagen spinning solution into the wet spinning experimental device, push the spinning solution out of the needle at a speed of 5mL / h through a syringe pump, and enter it into the coagulation bath with absolute ethanol as the coagulation agent. In the coagulation bath, the fibers obtained by the wet spinning process were fully shaped and then stretched and wound to obtain collagen fibers; wherein, the inner diameter of the syringe needle was 0.08mm, and the spinning temperature was 25°C. ...

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Abstract

The invention relates to a method for preparing collagen fiber through wet spinning. Firstly, I type collagen is dissolved in a NaAc / HAc buffering solution with the pH being 3.0 at the room temperature, a high-concentration collagen content collagen solution is obtained, vacuum pumping defoaming treatment is conducted, absolute ethyl alcohol is adopted as a coagulating bath, high-content collagenfiber is prepared through a wet spinning manner, the fiber is subjected to green crosslinking treatment, and the collagen fiber good in performance is obtained. Compared with the prior art, the NaAc / HAc buffering solution is adopted as the collagen green solvent, and the content of the collagen in the fiber is greatly increased; the absolute ethyl alcohol which is the coagulating bath not commonlyused in collagen wet spinning is adopted to prepare the collagen fiber, and ethyl alcohol is volatile and free of toxicity. The whole preparing method is moderate in condition and green in technologyand is simple and convenient to conduct. The prepared collagen fiber has good biocompatibility and excellent mechanical performance, meanwhile, the purpose that the degrading rate can be regulated and controlled can be achieved, and a new path is provided for collagen-based medical composite fiber preparing and application.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical materials, and in particular relates to a method for preparing collagen fibers by wet spinning. Background technique [0002] Collagen constitutes the skeleton of the extracellular matrix. As a biological macromolecular substance, it provides cells with certain tension resistance and elasticity, and plays a very important role in the living body. It has been widely used in the field of biomedical materials, such as Surgical sutures, artificial skin, collagen sponge, artificial blood vessels, etc., cosmetics and food industries, etc. However, collagen has a specific triple helix structure, and there are a large number of secondary bonds such as hydrogen bonds, ionic bonds, van der Waals forces and hydrophobic bonds inside the molecule, making it difficult to dissolve collagen in common solvents, which greatly restricts the application of collagen . [0003] At present, in the process of p...

Claims

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Application Information

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IPC IPC(8): D01F4/00D06M13/224D06M101/14
CPCD01F4/00D06M13/224D06M2101/14
Inventor 周媛媛李松赵雅光
Owner NORTH CHINA UNIV OF WATER RESOURCES & ELECTRIC POWER
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