Non-target biomarker high-throughput screening method based on pollutant metabolism disturbance

Abstract

The invention discloses a non-target biomarker high-throughput screening method based on pollutant metabolism disturbance, and belongs to the field of environmental exposure and health, and the methodcomprises the following steps: (1) extracting to obtain a to-be-detected extracting solution; (2) performing chromatographic analysis to obtain a spectrogram containing chromatographic peaks; (3) identifying and labeling pollutant characteristic peaks, taking chromatographic peaks except the pollutant characteristic peaks as potential metabolite characteristic peaks, and carrying out non-target labeling on the potential metabolite characteristic peaks; (4) establishing a linear regression model by taking the peak area of the potential metabolite characteristic peak as a dependent variable andthe peak area of the pollutant characteristic peak as an independent variable; (5) operating the model, carrying out non-target screening on the biomarkers, and preliminarily obtaining related biomarkers; and (6) identifying the preliminarily obtained primary and secondary spectrograms of the biomarker, and identifying the biomarker related to pollutant exposure. According to the method disclosedby the invention, the accuracy of biomarker screening is remarkably improved, and meanwhile, the flux of biomarker screening is improved.

Description

technical field [0001] The invention belongs to the field of environmental exposure and health, and in particular relates to a high-throughput screening method for non-target biomarkers based on pollutant metabolism disturbance. Background technique [0002] With the development of science and technology, people's living standards continue to improve, and the pollutants produced are also increasing. These substances are released into the environment and accumulate in the environment. Organisms are exposed to various environmental media, and pollutants in the environment may enter organisms through various channels such as touching, breathing, and eating, resulting in a transformation from external exposure to internal exposure. Exogenous environmental pollutants that enter organisms pose potential threats to organisms, and there is substantial evidence that exposure to specific chemicals may cause disease. [0003] Biomarkers are signal indicators for abnormal changes obser...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem of insufficient screening throughput and low accuracy existing in metabolomics research in the prior art, the screening method of the present invention first performs non-target screening of potential metabolites, and then gradually narrows the range to obtain a small range of metabolites. The number of biomarkers can ensure more comprehensive screening, achieve high-throughput, accurate screening and identification of biomarkers, and provide a scientific basis for toxicity prediction and pollutant risk assessment and control

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