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Combined extraction method of total flavonoids and total alkaloids from Qingpi and its application

A technology of total alkaloids and total flavonoids is applied in directions such as pharmaceutical combinations, medical preparations containing active ingredients, pharmaceutical formulations, etc., which can solve the problems of high cost, unenvironmental protection, time-consuming and other problems, and achieve the effect of simple process and avoidance of waste.

Active Publication Date: 2022-03-25
SHANGHAI JAKA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing methods all use resin column chromatography. In industrial production, resin column chromatography and resin regeneration treatment need to consume a lot of organic solvents, acids and alkalis, which is neither environmentally friendly nor labor-intensive.

Method used

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  • Combined extraction method of total flavonoids and total alkaloids from Qingpi and its application
  • Combined extraction method of total flavonoids and total alkaloids from Qingpi and its application
  • Combined extraction method of total flavonoids and total alkaloids from Qingpi and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Combined extraction of total alkaloids and total flavonoids from green peel:

[0049] Take 1kg of dried flaky green skin medicinal material, soak it in 10L deionized water for half an hour at room temperature, adjust the pH of the water to 3 with 20% hydrochloric acid, and extract it in a 40°C water bath for 2 hours to obtain about 7.5L of the extract. After filtration with a ceramic membrane, ultrafiltration was performed on a Millipore Labscale TFF ultrafiltration system with a cellulose filter membrane with a molecular weight cut-off of 3K, and about 3.1 L of the filtrate was collected. 3.1L of the filtrate was concentrated to about 0.3L under reduced pressure at 65°C, filtered with a Buchner funnel, the filtrate was collected, and freeze-dried to obtain 5g of total alkaloids from the green peel. The ultraviolet spectrum of the total alkaloid part of the present invention ( figure 1 ) and synephrine hydrochloride ( figure 2 ) is similar, confirming that total alka...

Embodiment 2

[0052] Combined extraction of total alkaloids and total flavonoids from green peel:

[0053] Take 1kg of dried flaky green skin medicinal material, soak it in 20L deionized water for half an hour at room temperature, adjust the pH of the water to 3.5 with 20% hydrochloric acid, and extract it in a 60°C water bath for 0.5h to obtain about 15.5L of the extract. After the liquid was filtered with a ceramic membrane, ultrafiltration was performed on a Millipore Labscale TFF ultrafiltration system with a cellulose filter membrane with a molecular weight cut-off of 3K, and about 7L of the filtrate was collected. 7L of the filtrate was concentrated to about 0.5L under reduced pressure at 65°C, filtered with a Buchner funnel, the filtrate was collected, and freeze-dried to obtain 5.2g of total alkaloids of the green peel.

[0054] The above medicinal residues were extracted with 10L of 80% ethanol at 70°C for 1 hour, and filtered to obtain about 8L of the extract. The extract was fil...

Embodiment 3

[0056] Evaluation of the inhibitory effect of different proportions of total alkaloids and total flavonoids of C. chinensis on TRPV1

[0057] The HaCaT cell line with high expression of TRPV1 was constructed to verify the analgesic effect of the above-mentioned total alkaloids and total flavonoids extracts. The total alkaloids and total flavonoids in Example 1 were calculated according to the ratios of 1:0, 10:1, 5:1, 2:1, 1:1, 1:2, 1:5, 1:10, and 0:1, respectively. Proportions are compounded, and a mother liquor with a concentration of 10% is prepared, and then the mother liquor is diluted 50 times as the test substance.

[0058] The test substance and Fluo-8 AM were incubated with the cells at 37°C for 30min, then capsaicin solution was added, and the fluorescence intensity was detected after incubation at 37°C for 15min, and the effect of the test substance on the influx of extracellular calcium ions was calculated by the fluorescence intensity The degree of inhibition, th...

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Abstract

The invention discloses a joint extraction method of total flavonoids and total alkaloids of Qingpi and its application; it comprises the following steps: S1 extracting plant raw materials by ultrasonication or heating with acid water, collecting the extract and dregs respectively; S2 extracting the step S1 The liquid is subjected to ultrafiltration and purification; S3 concentrates the filtrate of step S2 to a small volume, filters, and collects the filtrate to obtain the total alkaloids of Qingpi; S4 extracts the medicinal residue of step S1 with an alcohol solvent or heats it, and collects the extract; S5 The extract in S4 is purified by ultrafiltration; in S6, the filtrate in step S5 is concentrated to a small volume, and the pH is adjusted to between 2-4, filtered, and the solid is collected to obtain the total flavonoids of Qingpi. The total alkaloids and total flavonoids of Qingpi are compounded according to a certain ratio. The Qingpi extract has a significant antagonistic effect on the important receptor that mediates skin pain and itching - the transient receptor potential vanillic acid receptor 1, so it has a good effect. It has analgesic effect and can be used in the preparation of anti-allergic products.

Description

technical field [0001] The invention belongs to the technical field of extraction of Chinese herbal medicines, and relates to a combined extraction method of total flavonoids and total alkaloids from the green peel and application thereof; in particular to a combined extraction process of total alkaloids and total flavonoids from the green peel and the preparation of a combined extraction process of total flavonoids and total flavonoids from the green peel application in the product. Background technique [0002] 52% of consumers worldwide consider their skin to be sensitive. Sensitive skin is a common clinical problem skin: slight external stimuli can activate the skin's self-protection response, causing skin tingling, burning, itching, redness and other symptoms. The burning and tingling sensations are the result of overexpression of transient receptor potential vanilloid 1 (TRPV1, also known as capsaicin receptor), a highly potent Ca-mediated receptor. 2+ The inward cat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/752A61P29/00A61P37/08A61P17/04
CPCA61K36/752A61P29/00A61P37/08A61P17/04A61K2236/333A61K2236/55A61K2236/53A61K2236/51
Inventor 刘青辛英祥周利丹王新亮马毅刚齐秋月周艳卢伊娜
Owner SHANGHAI JAKA BIOTECH CO LTD
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