Konjac powder multienzyme hydrolysate and preparation method thereof
A technology of konjac refined flour and enzymatic hydrolysate is applied in the field of konjac refined flour multi-enzyme hydrolysate and its preparation, and can solve the problems of incomplete enzymatic hydrolysis, long enzymatic hydrolysis time, low utilization rate of konjac refined flour and the like
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[0022] The invention provides a preparation method of konjac powder multi-enzyme hydrolyzate, comprising the following steps:
[0023] (1) purifying the konjac fine powder raw material in alcohol to obtain wet konjac powder;
[0024] (2) Dealcoholizing the wet fine powder of konjac to obtain purified fine powder of konjac;
[0025] (3) The obtained purified konjac powder, water and enzyme are mixed and then enzymatically hydrolyzed to obtain konjac powder multi-enzyme hydrolyzate.
[0026] In the present invention, the konjac powder is preferably high-purity commercially available konjac powder.
[0027] In the present invention, the volume concentration of alcohol in the step (1) is preferably 55%-75%, more preferably 60%-70%, even more preferably 62%-68%.
[0028] In the present invention, the solid-liquid ratio of konjac powder raw material and alcohol in the step (1) is preferably 1g:3-5ml, more preferably 1g:3.5-4.5ml.
[0029] In the present invention, the purificatio...
Embodiment 1
[0044] Step 1: Purification: Purify the konjac fine powder raw material in alcohol with a volume concentration of 55%, and the solid-liquid ratio of the konjac fine powder raw material to alcohol is 1g:3ml, and obtain wet konjac fine powder after 1 hour of purification.
[0045] Step 2: Dealcoholization: Dealcoholization of wet konjac powder, the temperature of dealcoholization is 50°C, and purified konjac powder is obtained after dealcoholization;
[0046] Step 3: Enzymolysis: Take 15g of purified konjac powder and add 100mL of water, then add glucomannanase, cellulase and pectinase for enzymolysis, wherein the dosage of glucomannanase is 50u / g of purified konjac powder , the dosage of cellulase is 40u / g purified konjac powder, the dosage of pectinase is 0.03% of the total mass of the system, and the multi-enzyme hydrolyzate of konjac powder is obtained by enzymatic hydrolysis at 55°C for 2 hours.
[0047] The content of functional components (oligosaccharides) in the obtaine...
Embodiment 2
[0049] Step 1: Purification: Purify the raw material of konjac fine powder in alcohol with a volume concentration of 60%, the ratio of solid to liquid of konjac fine powder raw material and alcohol is 1g:4ml, and obtain wet konjac powder after purification for 1.5h.
[0050] Step 2: Dealcoholization: Dealcoholize the wet fine powder of konjac, the temperature of dealcoholization is 65°C, and obtain purified konjac fine powder after dealcoholization;
[0051] Step 3: Enzymolysis: Take 25g of purified konjac powder and add 100mL of water, then add glucomannanase, cellulase and pectinase for enzymolysis, wherein the dosage of glucomannanase is 60u / g of purified konjac powder , the dosage of cellulase is 50u / g purified konjac powder, the dosage of pectinase accounts for 0.04% of the total mass of the system, and the multi-enzyme hydrolyzate of konjac powder is obtained by enzymatic hydrolysis at 60°C for 2.5h.
[0052] The content of functional components (oligosaccharides) in the...
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