Unlock instant, AI-driven research and patent intelligence for your innovation.

Efficient blue-green algae organic matter resource comprehensive utilization method

A technology of organic matter and cyanobacteria, applied in the direction of organic fertilizer, biological organic part treatment, application, etc., can solve the problems of money investment, reduced economic benefits, limited degradation effect of algal toxins, etc., to achieve efficient treatment and bring economic benefits.

Inactive Publication Date: 2020-08-14
潘海峰 +4
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The scheme disclosed in the above patent still has certain defects: 1. The addition amount of cyanobacteria algae mud in the matrix is ​​relatively small, and bran or corn husks and fungus residue need to be purchased, so economic value cannot be generated, and additional money needs to be invested; 2. .The degradation effect on algae toxins is limited, and the mortality rate of the flat-horned soldier fly is high, and the economic benefits brought about are reduced

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] An efficient comprehensive utilization method of cyanobacteria organic matter resources, comprising the following steps:

[0034] S1. Cyanobacteria are fermented with compound degrading bacteria;

[0035] In this experiment, samples were taken from the surface water of Taihu Lake near Fisherman's Wharf in Huzhou, Zhejiang Province, and the water DNA was extracted with the Water DNA Kit Water DNA Extraction Kit (Omega). Using MEGA software to compare and analyze the key microcystin degradation gene mlrA sequence in the current NCBI database, and using Primer Premier5.0 to design two degenerate primers between sequences with high homology, and amplify by PCR , to detect whether there are microcystin-degrading bacteria in the water sample. Using the extracted water sample DNA as a template, primer 1 (5'TGCGCTATGGGKCAGATCC, where K stands for T or G 3') and primer 2 (5'GGTCAAACTTCTTGAGSAGCTG, where S stands for G or C 3') as primers, perform PCR reaction. The PCR reactio...

Embodiment 2

[0046] The difference from Example 1 is that in S3, domesticated black soldier fly larvae are used. During the process of feeding cyanobacteria, large larvae are selected (larvae with a body length of more than 2 cm after domestication and cultivation for 20 days) and enter the later stage. Prepupa, pupa, adult and egg-laying life cycle. After the obtained eggs are hatched by bran with a moisture content of 70% (hatching temperature is 28°C, hatching time is 3 days), they continue to be fed with cyanobacteria, and continue to select large larvae for a new round of screening. Repeatedly, a total of 5 rounds of acclimatization and screening were carried out.

[0047] The above-mentioned domesticated black soldier flies are fed according to the method of S3 in Example 1, the material reduction rate is 70.3%, the larval mortality rate is 4.1%, and the average body weight of the black soldier flies is 201 mg / worm, and the effect is relatively low. Significantly before domesticatio...

Embodiment 3

[0049] The difference from Example 1 is that the bran in S2 is replaced by rice bran, the dry matter ratio of cyanobacteria: rice bran is 2:6, and the cyanobacteria and bran are directly mixed, fed according to the method in S3, and fed by black soldier fly After transformation, the material reduction rate was 53.3%, the larval mortality rate was 5.8%, and the average body weight of black soldier flies was 163mg / worm.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of environmental protection, and relates to an efficient blue-green algae organic matter resource comprehensive utilization method. The method comprises the following steps: S1, fermenting blue-green algae by adopting a composite degrading strain; S2, uniformly mixing the fermented blue-green algae with auxiliary materials according to a dry matter ratio of 2: (4-6), and adjusting the water content to 62%-75%; S3, adding hermetia illucens larvae into the mixed material of the blue-green algae and the auxiliary materials according to the ten-thousandth proportion, and maintaining the temperature at 25-28 DEG C and the substrate moisture content at 62%-75% in the treatment period; and S4, separating the insects from the base material; and S5, carrying out stack retting on the base material at room temperature to obtain a decomposed bio-organic fertilizer. According to the method, the blue-green algae can be efficiently treated, algal toxins ofthe blue-green algae can be degraded, economic benefits can be brought, the death rate of larvae is 4.1% or below, and the average weight of hermetia illucens is at least 201 mg / insect.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and relates to a method for comprehensively utilizing cyanobacteria organic matter resources with high efficiency. Background technique [0002] In some nutrient-rich water bodies, some cyanobacteria often multiply in large numbers in summer, and form a layer of blue-green cyanobacteria blooms on the water surface and have a fishy smell. For "green tide". Green tides cause water quality to deteriorate, and in severe cases deplete the oxygen in the water and cause the death of fish. What's more serious is that some species of cyanobacteria (such as Microcystis) also produce microcystins (MCs for short), and about 50% of green tides contain a large amount of MCs. [0003] Microcystins (MCs for short) are a class of biologically active cyclic heptapeptide compounds with a molecular weight of about 1000Da. More than 90 isomers have been found so far. Among these identified isomers, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C05F15/00C05F17/20C05F17/50A23K10/20A01K67/033
CPCA01K67/033A23K10/20C05F5/002C05F17/20C05F17/50C05F11/00C05F11/08Y02W30/40
Inventor 潘海峰鲍文娜董骏豪傅晨梅贺仲宪
Owner 潘海峰