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A nano-drug that triggers tumor immunogenic death by near-infrared light and its preparation method and application

A near-infrared light, nano-drug technology, applied in the field of medicine, to achieve the effect of being beneficial to biological applications, low energy, and inhibiting transfer

Active Publication Date: 2021-08-13
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the use of NIR PDT for tumor immunotherapy has not been reported, therefore, it is now worth developing nanophotosensitizers for NIR

Method used

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  • A nano-drug that triggers tumor immunogenic death by near-infrared light and its preparation method and application
  • A nano-drug that triggers tumor immunogenic death by near-infrared light and its preparation method and application
  • A nano-drug that triggers tumor immunogenic death by near-infrared light and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, the synthesis of gold bipyramid nanoparticles:

[0048] (1) Synthesis of decahedron seeds:

[0049] Dissolve 0.005mol of polydiallyldimethylammonium chloride (Mw=100000, 20wt%) in 200mL of polyethylene glycol, mix well, mix 0.0001mol of chloroauric acid and 0.0003mol of silver nitrate, 0.0000002mol of trichloro Iron oxide was added to the above solution. After passing oxygen for 30 minutes, react at 200° C. for 4 hours to obtain a decahedron seed solution.

[0050] (2) Synthesis of gold bipyramid nanoparticles:

[0051] Add 0.0004mol hydroquinone and 0.0001mol chloroauric acid to 200mL decahedron seed solution. React in an oven at 50°C for 15 hours to obtain a gold bipyramid nanoparticle ethylene glycol dispersion, centrifuge it at 13,000 rpm for 30 minutes, discard the supernatant, add pure water to wash three times to obtain an aqueous solution of gold bipyramid nanoparticles.

[0052] The appearance of the prepared gold bipyramid is as follows: figur...

Embodiment 2

[0054] Embodiment 2, the synthesis of methoxy polyethylene glycol dopamine (mPEG-DA)

[0055] (1) 2g of methoxypolyethylene glycol carboxyl (mPEG-COOH) and toluene are azeotropically dewatered, and DMF is dewatered with a dewatering device;

[0056] (2) Combine the above-mentioned treated mPEG-COOH with 307mg 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl), 92mg N-hydroxysuccinyl Amine (NHS) was dissolved in 50mL of DMF, activated for two hours under ice bath conditions, 378mg of dopamine (DA) was added to the above solution to react for 24 hours, after the reaction was complete, dialyzed with pure water to obtain dialysate;

[0057] (3) The dialysate was freeze-dried to obtain mPEG-DA gray powder.

Embodiment 3、10

[0058] Embodiment 3, the synthesis of 10nm titanium dioxide layer thickness coated gold bipyramid nanoparticles

[0059] (1) Disperse 100mg of gold bipyramid nanoparticles in 200mL (2g / L) polystyrene sulfonate sodium (PSS) buffer (12mM sodium chloride), adsorb and modify at room temperature for 24h, and then centrifuge at 12000rpm After 20 minutes, the free PSS was discarded, and washed three times with pure water to obtain PSS-modified gold bipyramidal nanoparticles;

[0060] (2) Add PSS-modified gold bipyramid nanoparticles to 35mL0.5% titanium trichloride hydrochloric acid solution, then add 7mL0.1M sodium bicarbonate solution dropwise to the above solution, and ultrasonically react under ice bath conditions Centrifuge for 2 hours at 8000rpm for 20min, and wash with pure water for 3 times to obtain titanium dioxide-coated gold bipyramidal nanoparticles.

[0061] The appearance of the prepared titania-coated gold bipyramids is as follows: image 3 as shown, image 3 It is...

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Abstract

The invention provides a nano-medicine for near-infrared light-triggered tumor immunogenic death, comprising gold nanoparticles, a titanium dioxide layer and a polymer layer; the titanium dioxide layer is coated on the surface of the gold nanoparticles, and the polymer layer is coated on the surface of the titanium dioxide layer. The above-mentioned nanomedicine generates active oxygen groups under the irradiation of near-infrared light, which causes the immunogenic death of tumors and inhibits subsequent tumor metastasis. Compared with visible light, the near-infrared light used has the advantage of low energy, which reduces the damage to biological tissues, has deeper tissue penetration, and can better treat deep tumors. In addition, the nanomedicine provided by the invention has good stability and dispersibility, which is beneficial to biological applications. Simultaneously, the preparation process is simple, the synthesis condition at room temperature is mild, and it has the possibility of large-scale production, and has the potential of industrial and practical application.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a nano-medicine for triggering tumor immunogenic death by near-infrared light, a preparation method and application thereof. Background technique [0002] Cancer is a malignant disease in which cells proliferate uncontrollably and even spread to the whole body. It has become the most important problem that endangers the health of residents all over the world. At the same time, after tumor metastasis, there is a problem of low survival rate and difficult treatment. In recent years, such as PD-1 / PDL-1 antibody, chimeric antigen receptor therapy (CART) has made great progress in anti-tumor, and immunotherapy occupies an increasingly important position in the field of tumor treatment. Photodynamic therapy (PDT) has been clinically proven to treat tumors. Tumor cells are killed by ROS generated by photosensitizers, causing tumor immunogenic death (ICD), recruiting initial immune effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/10A61K47/02A61P35/00
CPCA61K9/5115A61K9/5146A61K41/0057A61P35/00
Inventor 王育才唐新锋杭立峰马寅初
Owner UNIV OF SCI & TECH OF CHINA
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