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Cardiovascular implant for regulating and controlling immune response and promoting intimal regeneration and preparation method of cardiovascular implant

An immune response and cardiovascular technology, applied in the field of biomedicine, to achieve the effect of promoting mobilization and proliferation, promoting early endothelialization, and promoting inflammation regression

Active Publication Date: 2020-08-18
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a cardiovascular implant that regulates the immune response in situ and promotes intima regeneration. The cardiovascular implant can effectively prevent the occurrence of problems such as thrombosis and regeneration difficulties, and improve the success rate of artificial blood vessel transplantation.

Method used

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  • Cardiovascular implant for regulating and controlling immune response and promoting intimal regeneration and preparation method of cardiovascular implant
  • Cardiovascular implant for regulating and controlling immune response and promoting intimal regeneration and preparation method of cardiovascular implant
  • Cardiovascular implant for regulating and controlling immune response and promoting intimal regeneration and preparation method of cardiovascular implant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Cardiovascular implants modified by simple CD133 aptamers

[0067] Preparation of natural decellularized vascular matrix material: Under sterile conditions, extract the common carotid artery from 250-300g SD rats, wash the blood with normal saline, separate and remove the outer connective tissue of the common carotid artery, and then cut it into a length of 0.5- Small blood vessels of 1 cm (generally, tissue engineered blood vessels with a length of 0.5-20 cm can meet the needs of clinical applications such as blood vessel transplantation, and the transplanted objects of the cardiovascular implants are rats, so small blood vessels with a length of 0.5-1 cm are used as Preferably), dilute trypsin with M199 medium to a concentration of 0.05% and digest blood vessels at 37°C for 30 minutes to remove cells, then use RNase, DNase and fat to remove nucleic acid and fat to obtain a vascular matrix material with only collagen and elastic fibers . Wherein, the calibe...

Embodiment 2

[0072] Example 2: Nano-sustained-release particles modified and surface-modified cardiovascular implants

[0073] In this example, nano-sustained-release particle modification and surface modification were performed on the cardiovascular implant body, but no CD133 aptamer modification was performed on the cardiovascular implant body, as follows:

[0074] Carry out the modification of slow-release nanoparticles on the body of the cardiovascular implant, mix 100ng / ml axon guidance molecule-1 (R&D, USA) and chitosan at a molar mass ratio of 1:2, and adjust the pH value of the solution 5.1-6.0, slowly add 0.15% TPP (sodium tripolyphosphate) during stirring, after continuous stirring to obtain a milky white liquid, centrifuge at 4°C for 10 minutes, wash the precipitate for 3 times with double distilled water, and then freeze-dry to obtain nano sustained-release particles. At 4°C, under the condition of 120rpm, use 0.1% (w / v) slow-release nanoparticles and 0.4% collagen solution to ...

Embodiment 3

[0080] Example 3: Triple Modified Cardiovascular Implants

[0081] In this example, the preparation method of the natural decellularized vascular matrix material and the cardiovascular implant body is the same as in Example 1, the difference is that the cardiovascular implant body is triple modified in this example, and the specific conditions are as follows:

[0082] CD133 aptamer anchoring modification: 5mM EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) was used to incubate the collagenous vascular matrix material (i.e. cardiovascular implant object body) for cross-linking for 24 h, and then washed 3 times with PBS. PEI / gold nanoparticles are synthesized by a one-step method. 1% PEI solution (polyethyleneimine) (1.44ml) is slowly added into 14mM or 28mM HAuCl4 aqueous solution (25ml), and stirred at room temperature for 24h to form low density and high density of PEI / gold nanoparticles. Then, centrifuge at 20000g / min at 4°C, wash with distilled water thr...

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Abstract

The invention belongs to the technical field of biological medicines, and relates to a cardiovascular implant and a preparation method thereof, in particular to a cardiovascular implant for regulatingimmune response in situ and promoting intimal regeneration and a preparation method thereof. The cardiovascular implant comprises a cardiovascular implant body, and the cardiovascular implant body ismodified with a CD133 aptamer and sustained-release nanoparticles. The cardiovascular implant body is modified with the CD133 aptamer and the sustained-release nanoparticles containing the nerve axonguidance molecule-1, the immune response is regulated and controlled in situ and intimal regeneration is promoted, thrombosis is effectively overcome, and long-term unobstructed blood vessels are promoted. Especially for patients with clinically inflammatory basal lesions, the invention provides the cardiovascular implant which is simple, convenient and rapid to prepare, wide in applicability andcapable of regulating excessive inflammatory reaction in situ and promoting intimal regeneration to maintain long-term functions and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a cardiovascular implant and a preparation method thereof, in particular to a cardiovascular implant for regulating immune response and promoting intima regeneration and a preparation method thereof. Background technique [0002] Cardiovascular disease is the number one disease that threatens human health. Every year 7.3 million people die from ischemic heart disease worldwide, ranking first among all diseases. Therefore, the demand for cardiovascular implants is increasing. Among them, bioartificial blood vessels are the development direction of vascular implants for coronary artery bypass grafting, hemodialysis and peripheral vascular occlusion. Moreover, the construction of complex tissues and organs such as liver, kidney, lung, and islets of pancreas also requires vascularization, leading to a further increase in the demand for bioartificial blood vessels. The immune respon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/34A61L27/28A61L27/54
CPCA61L27/507A61L27/28A61L27/54A61L27/34A61L2300/252A61L2300/624A61L2300/232A61L2300/41A61L2300/42C08L5/08
Inventor 曾文朱楚洪李彦朝薛方超
Owner ARMY MEDICAL UNIV
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