Method for reducing secretion amount of lactobacillus rhamnosus surface substance
A technology of Lactobacillus rhamnosus and secretion volume, applied in the field of microorganisms, can solve the problems of pH dynamic balance damage, protein denaturation inactivation, reduction of Lactobacillus rhamnosus, etc., and achieves the effect of reducing the difficulty of collection and reducing the secretion volume.
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[0051] Example 1: Preparation of Lactobacillus rhamnosus powder
[0052] Specific steps are as follows:
[0053] Use an inoculating loop to dip the Lactobacillus rhamnosus CCFM1060 in the bacteria-preserving tube and streak it on the MRS solid medium. Incubate at 37°C for 36 hours to obtain a single colony; pick a single colony and inoculate it in the MRS liquid medium, 37 Incubate at a constant temperature for 12 hours to obtain a seed solution; inoculate the seed solution into medium A at an inoculum amount of 2% (v / v), and culture at 37°C and pH 5 for 6h, 9h, 12h, respectively, to obtain the culture Solution 1~3; Centrifuge the culture solution 1~3, and collect bacteria 1~3;
[0054] Among them, the components of medium A are: arabinose 20g / L, yeast extract powder FM52825g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L and Tween-801g / L.
[0055] Take some cells 1-3 and test the surface protein content and capsular polysaccharide content of cells 1-3 (see Table 1 for test ...
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[0066] Example 2: Preparation of Lactobacillus rhamnosus powder
[0067] Specific steps are as follows:
[0068] On the basis of Example 1, the Lactobacillus rhamnosus CCFM1060 was replaced with Lactobacillus rhamnosus CCFM1064, Lactobacillus rhamnosus CCFM1068, and Lactobacillus rhamnosus CCFM1070, respectively, to obtain bacterial cells 4-12 and rhamnose milk Bacillus powder 4-12.
[0069] Detect the surface protein content and capsular polysaccharide content on the surface of bacteria 4-12 (see Table 3 for the test results), detect the number of viable Lactobacillus rhamnosus in the Lactobacillus rhamnosus powder 4-12, and calculate the rat The lyophilized survival rate of Lactobacillus rhamnosus in 4-12 Lactobacillus rhamnosus powder (see Table 4 for the test results).
[0070] It can be seen from Tables 3 to 4 that when cultured for 9 hours or more, the surface protein content and capsular polysaccharide content on the surface of the bacteria 4-12 are lower, and the Lactobacillu...
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[0075] Example 3: Preparation of Lactobacillus rhamnosus powder
[0076] Specific steps are as follows:
[0077] On the basis of Example 1, the carbon source in the medium A was replaced with glucose, galactose, glucose or sucrose, respectively, to obtain cells 13-16 and Lactobacillus rhamnosus powder 13-16.
[0078] Detect the surface protein content and capsular polysaccharide content of the cells 13-16 (see Table 5 for the test results), detect the number of viable Lactobacillus rhamnosus in the Lactobacillus rhamnosus powder 13-16, and calculate the rat The lyophilized survival rate of Lactobacillus rhamnosus in the 13-16 Lactobacillus rhamnosus powder (see Table 6 for test results).
[0079] It can be seen from Tables 1 to 2 and Tables 5 to 6 that the choice of carbon source has an effect on the surface protein content and capsular polysaccharide content of the bacterial surface, as well as the number and number of viable Lactobacillus rhamnosus in the Lactobacillus rhamnosus pow...
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