Hydrogel injection of self-crosslinking hyaluronic acid and composite collagen of self-crosslinking hyaluronic acid and application of hydrogel injection

Abstract

The invention discloses a hydrogel injection of self-crosslinking hyaluronic acid and composite collagen of the self-crosslinking hyaluronic acid and an application of the hydrogel injection. The structure of the injection is shown as a formula I. According to the invention, a sulfydryl modified natural material or a synthetic polymer and a double bond-containing compound modified natural materialor a synthetic polymer are subjected are mixed with a protein natural polymer material or a derivative of the protein natural polymer material without participation of other cross-linking agents forloading cells, drugs, protein active factors and other bioactive substances, and the injectable double-crosslinking composite hydarogel injection loaded with bioactive substances is prepared under action of a Michael addition reaction. The modification method provided by the invention is simple and feasible, and the obtained modifier has good biocompatibility. The injection type double-crosslinking composite hydrogel has good reaction rate controllability, mechanical properties and biocompatibility, a good foundation for application of the injection type double-crosslinking composite hydrogelin injection type hydrogel is laded, and an application prospect in the aspect of bone / cartilage defect filling is also exhibited.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, specifically a hydrogel injection of self-crosslinked hyaluronic acid and its composite collagen and its application. The injection can be used for bone / cartilage regeneration and repair, filling bone / cartilage defects It has a good application prospect. Background technique [0002] Tissue engineering has developed by leaps and bounds since it was first proposed. Not only have various tissue-engineered tissues been constructed in animals, but they have also been widely used clinically and have achieved lasting curative effects. Tissue engineering technology can avoid immune rejection and insufficient donor sources in traditional autologous or allogeneic tissue and organ transplantation, and it is hopeful to realize tissue and organ reconstruction to solve clinical difficulties. The study of tissue engineering includes methods and techniques for seeding cells, scaffolding materials,...

AI Technical Summary

Problems solved by technology

However, most of the early scaffold materials used synthetic polymers. Although they were biocompatible, these materials were not beneficial to cells and had no cell adhesion sites, resulting in the failure of cells to grow normally.

[0003] As an extracellular matrix, hyaluronic acid has good biocompatibility and high water content, but its structure is simple and its function is single. At the same time, its degradation rate is too fast and its mechanical properties are poor, which cannot meet the application requirements.

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